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D236N
site-directed mutagenesis, D236 is the first aspartic acid in the canonical GT-A DxD catalytic motif, and mutation to the isosteric asparagine results in the loss of activity
D280N
site-directed mutagenesis, inactive mutant
H389A
site-directed mutagenesis, inactive mutant
M16/O16
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chimeric Mnn9p-Och1p protein, a peptide from the first to the 16th amino acids of Mnn9p is fused with a polypeptide from the 16th to the 480th amino acids of Och1p
M33/O37
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chimeric Mnn9p-Och1p protein, a peptide from the first to the 33th amino acids of Mnn9p is fused with a polypeptide from the 37th to the 480th amino acids of Och1p
M93/O80
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chimeric Mnn9p-Och1p protein, a peptide from the first to the 93th amino acids of Mnn9p is fused with a polypeptide from the 80th to the 480th amino acids of Och1p
R209A
site-directed mutagenesis, inactive mutant
D236N
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site-directed mutagenesis, D236 is the first aspartic acid in the canonical GT-A DxD catalytic motif, and mutation to the isosteric asparagine results in the loss of activity
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D280N
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site-directed mutagenesis, inactive mutant
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H389A
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site-directed mutagenesis, inactive mutant
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R209A
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site-directed mutagenesis, inactive mutant
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W266stop
inactive mutant strain 7X4 with a nonsense mutation resulting in a truncated Och1p, temperature- and hygromycin B-sensitive, no synthesis of outer chains on acid phosphatase
additional information
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Mnn9 mutant strain
additional information
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och1 mutants, och1 mutation causes a complete loss of the alpha-1,6-polymannose outer chain
additional information
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DELTAmnn9 mutant with N-glycosylation defect, null mutant, chimera Mnn9 proteins
additional information
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strain with deletion of the OCH1 gene
additional information
construction of a yeast DELTAMNN9 enzyme deletion strain, presence of inactive full-length ScMnn9 protein partially rescues the DELTAMNN9 phenotype
additional information
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construction of a yeast DELTAMNN9 enzyme deletion strain, presence of inactive full-length ScMnn9 protein partially rescues the DELTAMNN9 phenotype
additional information
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Mnn9 mutant strain
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additional information
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construction of a yeast DELTAMNN9 enzyme deletion strain, presence of inactive full-length ScMnn9 protein partially rescues the DELTAMNN9 phenotype
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additional information
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och1 mutants, och1 mutation causes a complete loss of the alpha-1,6-polymannose outer chain
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additional information
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och1+ gene deficient strain shows loss of mannose transfer from GDP-mannose to Man9GlcNAc2-PA and loss of high-mannose type sugar chain glycosylation, och1 mutant that does not synthesize the outer chains containing mannose on acid phosphatase
additional information
disruption mutant of och1+: viable, slow-growing phenotype, temperature- and hygromycin B-sensitive, no synthesis of outer chains on acid phosphatase
additional information
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disruption mutant of och1+: viable, slow-growing phenotype, temperature- and hygromycin B-sensitive, no synthesis of outer chains on acid phosphatase
additional information
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och1+ gene deficient strain shows loss of mannose transfer from GDP-mannose to Man9GlcNAc2-PA and loss of high-mannose type sugar chain glycosylation, och1 mutant that does not synthesize the outer chains containing mannose on acid phosphatase
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additional information
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OCH1 and Anl1 deletion strains, increased sensitivity to sodium dodecyl sulfate, and to Calcofluor White