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2.4.1.20: cellobiose phosphorylase

This is an abbreviated version!
For detailed information about cellobiose phosphorylase, go to the full flat file.

Word Map on EC 2.4.1.20

Reaction

cellobiose
+
phosphate
=
alpha-D-glucose 1-phosphate
 +
D-glucose

Synonyms

Cbp, CbpA, cellobiose phosphorylase A, cellobiose:orthophosphate alpha-D-glucosyltransferase, CPase, CuCbP, GH94 cellobiose phosphorylase, lactose phosphorylase, ra2122, THA_1941, Tm_1848

ECTree

     2 Transferases
         2.4 Glycosyltransferases
             2.4.1 Hexosyltransferases
                2.4.1.20 cellobiose phosphorylase

Specific Activity

Specific Activity on EC 2.4.1.20 - cellobiose phosphorylase

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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.033
purifed recombinant wild-type enzyme, substrate lactose
0.1
purifed recombinant mutant A397V/T508A/A512T/D557N/N667T/G681S, substrate lactose
0.16
with D-maltose as substrate, at pH 7.5 and 75°C
0.171
purifed recombinant mutant T508I/N667A, substrate lactose
0.249
purifed recombinant mutant T508A/N667T, substrate lactose
0.27
with D-galactose as substrate, at pH 7.5 and 75°C
0.35
with D-xylose as substrate, at pH 7.5 and 75°C
0.38
with D-mannose as substrate, at pH 7.5 and 75°C
0.85
with 2-deoxy-D-glucose as substrate, at pH 7.5 and 75°C
1.08
with D-glucosamine as substrate, at pH 7.5 and 75°C
1.35
purifed recombinant mutant A397V/T508A/A512T/D557N/N667T/G681S, substrate cellobiose
1.71
purifed recombinant mutant T508I/N667A, substrate cellobiose
1.86
with D-glucose as substrate, at pH 7.5 and 75°C
14.4
-
pH 6.6, 30°C
2.61
purifed recombinant mutant T508A/N667T, substrate cellobiose
2.66
-
purified enzyme
20
-
purified enzyme
27.4
-
purified enzyme
33.3
-
purified enzyme
40.8
-
purified enzyme
49.98
with gentiobiose as substrate, at pH 7.5 and 75°C
58.61
with cellobiose as substrate, at pH 7.5 and 75°C
7.78
purifed recombinant wild-type enzyme, substrate cellobiose
additional information