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2.4.1.155: alpha-1,6-mannosyl-glycoprotein 6-beta-N-acetylglucosaminyltransferase

This is an abbreviated version!
For detailed information about alpha-1,6-mannosyl-glycoprotein 6-beta-N-acetylglucosaminyltransferase, go to the full flat file.

Word Map on EC 2.4.1.155

Reaction

UDP-N-acetyl-alpha-D-glucosamine
+
beta-D-GlcNAc-(1->2)-[beta-D-GlcNAc-(1->4)]-alpha-D-Man-(1->3)-[beta-D-GlcNAc-(1->2)-alpha-D-Man-(1->6)]-beta-D-Man-(1->4)-beta-D-GlcNAc-(1->4)-beta-D-GlcNAc-N-Asn-[protein]
=
UDP
 +
beta-D-GlcNAc-(1->2)-[beta-D-GlcNAc-(1->4)]-alpha-D-Man-(1->3)-[beta-D-GlcNAc-(1->2)-[beta-D-GlcNAc-(1->6)]-alpha-D-Man-(1->6)]-beta-D-Man-(1->4)-beta-D-GlcNAc-(1->4)-beta-D-GlcNAc-N-Asn-[protein]

Synonyms

acetylglucosaminyltransferase, uridine diphosphoacetylglucosamine-alpha-mannoside beta1-6-UDP-N-acetylglucosamine:alpha-mannoside-beta1,6 N-acetylglucosaminyltransferase, alpha-1,3(6)-mannosylglycoprotein beta-1,6-N-acetylglucosaminyltransferase, alpha-1,6-mannosyl-glycoprotein 6-beta-N-acetylglucosaminyltransferase, alpha-mannoside beta-1,6-N-acetylglucosaminyltransferase, beta N-acetylglucosaminyltransferase V, beta-1,6-N-acetylglucosaminyltransferase V, beta1, 6-N-acetylglucosaminyltransferase V, beta1,6-N-acetylglucosaminyltransferase, beta1,6-N-acetylglucosaminyltransferase V, beta1,6-N-acetylglucosaminyltransferase-V, Glc-NAcT-V, GlcNAcT-V, GlcNAcTase-V, glycosyltransferase, GnT-IV, GnT-IX, GnT-V, GnT-Va, GnT-VB, Golgi beta-1,4-mannosyl-glycoprotein 4-beta-N-acetylglucosaminyltransferase V, Golgi UCD-GlcNAc:N-acetylglucosaminyltransferase 5, mannoside acetylglucosaminyltransferase 5, Mgat5, N-acetylglucosaminyltransferase V, N-acetylglucosaminyltransferase Va, N-acetylglucosaminyltransferase-V, N-acetylglucosaminyltransferase-Va, N-acetylglucosaminyltransferase-Vb, N-acetylglucsaminyltransferase V, UDP-N-acetylglucosamine:a-mannoside beta-1,6-N-acetylglucosaminyltransferase

ECTree

     2 Transferases
         2.4 Glycosyltransferases
             2.4.1 Hexosyltransferases
                2.4.1.155 alpha-1,6-mannosyl-glycoprotein 6-beta-N-acetylglucosaminyltransferase

Cloned

Cloned on EC 2.4.1.155 - alpha-1,6-mannosyl-glycoprotein 6-beta-N-acetylglucosaminyltransferase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
a recombinant vector MGAT5-pCXNneo is constructed for transfection of WiDr cells
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fusion with signal peptide from baculoviral protein to permit secretion into medium, BVVdelta73 and BVV delta188
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gene expression profiling of cells is conducted by DNA microarray analysis
gene MGAT5, overexpression in CHO cells and Lec4 cells from Cricetulus griseus
gene MGAT5, quantitative real-time PCR enzyme expression analysis
gene MGAT5, real-time RT-PCR enzyme expression analysis, establishing of human melanoma cell line WM-266-4 with induced stable overexpression of GnT-V, the cells show ncreased amounts of bisected and beta1-6 branched N-glycans on melanoma cell adhesion molecule MCAM/MUC18, but do not display significant differences in viability and capabilities to migrate through an endothelial layer, coexpression with MGAT3 gene, EC 2.4.1.144
gene MGAT5, recombinant expression of enzyme Gnt-V in CNE-1 and CNE-2 cells using the lentiviral transfection method
gene MGAT5, recombinant tet-inducible expression of Flag-tagged GlcNAcT-V in HeLa and HEK293 cells. Tet-induced Mgat5 HEK293 cells display a 2fold increase in tri-antennary and a 2fold increase in tetraantennary N-glycans
Huh7 and HepG2 cells are transiently transfected by both Mgat5 expression vector plasmid and empty vector plasmid as a control
into the pcDNA3 vector for transfection of rat pheochromocytoma cells, PC12
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into the pGEM-Teasy vector and subcloned into the pcDNA3 expression vector
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MKN45 cells are transfected with GnT-III, GnT-V, or both, respectively
-
stable overexpression of N-acetylglucosaminyltransferase V (GnT-V) in breast cancer MCF-10A cell line
transfection in HEK-293 cells
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transfection into 7721 cells
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wild type enzyme and several N- and C-terminal deletions, fusion to protein A from Staphylococcus aurus
-