2.1.1.187: 23S rRNA (guanine745-N1)-methyltransferase
This is an abbreviated version!
For detailed information about 23S rRNA (guanine745-N1)-methyltransferase, go to the full flat file.
Word Map on EC 2.1.1.187
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2.1.1.187
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rrnas
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macrolide
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methyltransferases
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tylosin
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fradiae
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pneumoniae
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stem-loops
- 2.1.1.187
- rrnas
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macrolide
- methyltransferases
- tylosin
- fradiae
- pneumoniae
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stem-loops
Reaction
Synonyms
23S rRNA m1G745 methyltransferase, 23S rRNA:m1G745 methyltransferase, ribosomal RNA(m1G)-methylase, RlmA, RlmA(I), RlmAI, RlmAI methyltransferase, rRNA large subunit methyltransferase I, rRNA(m1G)methylase, YebH
ECTree
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Substrates Products
Substrates Products on EC 2.1.1.187 - 23S rRNA (guanine745-N1)-methyltransferase
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REACTION DIAGRAM
S-adenosyl-L-methionine + guanine745 in 23S rRNA
S-adenosyl-L-homocysteine + N1-methylguanine745 in 23S rRNA
S-adenosyl-L-homocysteine + N1-methylguanine745 in 23S rRNA
Gram-negative 23 S rRNAs are methylated at G745. 23S rRNA of Gram-positives is methylated at G748. The position of an RNA methylation defines a sharp division between the Gram-negative and Gram-positive bacteria. Specificity of methylation is determined solely by the methyltransferase enzyme and is independent of the origin of the rRNA substrate
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S-adenosyl-L-methionine + guanine745 in 23S rRNA
S-adenosyl-L-homocysteine + N1-methylguanine745 in 23S rRNA
Gram-negative 23 S rRNAs are methylated at G745. 23S rRNA of Gram-positives is methylated at G748. The position of an RNA methylation defines a sharp division between the Gram-negative and Gram-positive bacteria. Specificity of methylation is determined solely by the methyltransferase enzyme and is independent of the origin of the rRNA substrate
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?
S-adenosyl-L-methionine + guanine745 in 23S rRNA
S-adenosyl-L-homocysteine + N1-methylguanine745 in 23S rRNA
Gram-negative 23 S rRNAs are methylated at G745. 23S rRNA of Gram-positives is methylated at G748. The position of an RNA methylation defines a sharp division between the Gram-negative and Gram-positive bacteria. Specificity of methylation is determined solely by the methyltransferase enzyme and is independent of the origin of the rRNA substrate. Azotobacter vinelandii shows intrinsic 23S rRNA methylation at G745. No methylation is determined with the recombinant protein in vivo or in vitro
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?
S-adenosyl-L-methionine + guanine745 in 23S rRNA
S-adenosyl-L-homocysteine + N1-methylguanine745 in 23S rRNA
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Gram-negative 23 S rRNAs are methylated at G745. 23S rRNA of Gram-positives is methylated at G748. The position of an RNA methylation defines a sharp division between the Gram-negative and Gram-positive bacteria. Specificity of methylation is determined solely by the methyltransferase enzyme and is independent of the origin of the rRNA substrate. Erwinia crysanthemi shows intrinsic 23S rRNA methylation at G745. No methylation is determined with the recombinant protein in vivo or in vitro
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S-adenosyl-L-methionine + guanine745 in 23S rRNA
S-adenosyl-L-homocysteine + N1-methylguanine745 in 23S rRNA
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?
S-adenosyl-L-methionine + guanine745 in 23S rRNA
S-adenosyl-L-homocysteine + N1-methylguanine745 in 23S rRNA
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?
S-adenosyl-L-methionine + guanine745 in 23S rRNA
S-adenosyl-L-homocysteine + N1-methylguanine745 in 23S rRNA
Gram-negative 23 S rRNAs are methylated at G745. 23S rRNA of Gram-positives is methylated at G748. The position of an RNA methylation defines a sharp division between the Gram-negative and Gram-positive bacteria. Specificity of methylation is determined solely by the methyltransferase enzyme and is independent of the origin of the rRNA substrate
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-
?
S-adenosyl-L-methionine + guanine745 in 23S rRNA
S-adenosyl-L-homocysteine + N1-methylguanine745 in 23S rRNA
the methylation at guanine745 is confined to Gram-negative bacteria
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S-adenosyl-L-methionine + guanine745 in 23S rRNA
S-adenosyl-L-homocysteine + N1-methylguanine745 in 23S rRNA
methylated guanines are located in hairpin 35, in domain II of prokaryotic 23S rRNA. RrmA possesses two regions that may be responsible for specific interactions with their target nucleic acid sequences: a putative Zn-finger domain in the N-terminus and the variable domain close to the C-terminus, which indicates that the enzyme exhibits the primary structural organization distinct from other nucleic acid MTases, despite sharing the common catalytic domain
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S-adenosyl-L-methionine + guanine745 in 23S rRNA
S-adenosyl-L-homocysteine + N1-methylguanine745 in 23S rRNA
methylation of the N1 position of nucleotide G745 in hairpin 35 of Escherichia coli 23S ribosomal RNA. Progressive truncation of the rRNA substrate shows that structures in stem-loops 33, 34 and 35 are required for methylation by RrmA. Multiple contacts between nucleotides in these stem-loops and RrmA are confirmed in footprinting experiments. No other RrmA contact is evident elsewhere in the rRNA. The RrmA contact sites on the rRNA are inaccessible in ribosomal particles and, consistent with this, 50S subunits or 70S ribosomes are not substrates for RrmA methylation. Methylate their target nucleotides only in the free RNA
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?
S-adenosyl-L-methionine + guanine745 in 23S rRNA
S-adenosyl-L-homocysteine + N1-methylguanine745 in 23S rRNA
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Gram-negative 23 S rRNAs are methylated at G745. 23S rRNA of Gram-positives is methylated at G748. The position of an RNA methylation defines a sharp division between the Gram-negative and Gram-positive bacteria. Specificity of methylation is determined solely by the methyltransferase enzyme and is independent of the origin of the rRNA substrate. Enterobacter aerogenes shows intrinsic 23S rRNA methylation at G745. No methylation is determined with the recombinant protein in vivo or in vitro
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?
S-adenosyl-L-methionine + guanine745 in 23S rRNA
S-adenosyl-L-homocysteine + N1-methylguanine745 in 23S rRNA
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Gram-negative 23 S rRNAs are methylated at G745. 23S rRNA of Gram-positives is methylated at G748. The position of an RNA methylation defines a sharp division between the Gram-negative and Gram-positive bacteria. Specificity of methylation is determined solely by the methyltransferase enzyme and is independent of the origin of the rRNA substrate. Proteus mirabilis shows intrinsic 23S rRNA methylation at G745. No methylation is determined with the recombinant protein in vivo or in vitro
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?
S-adenosyl-L-methionine + guanine745 in 23S rRNA
S-adenosyl-L-homocysteine + N1-methylguanine745 in 23S rRNA
-
Gram-negative 23 S rRNAs are methylated at G745. 23S rRNA of Gram-positives is methylated at G748. The position of an RNA methylation defines a sharp division between the Gram-negative and Gram-positive bacteria. Specificity of methylation is determined solely by the methyltransferase enzyme and is independent of the origin of the rRNA substrate. Pseudomonas fluorescens shows intrinsic 23S rRNA methylation at G745. No methylation is determined with the recombinant protein in vivo or in vitro
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-
?
S-adenosyl-L-methionine + guanine745 in 23S rRNA
S-adenosyl-L-homocysteine + N1-methylguanine745 in 23S rRNA
-
Gram-negative 23 S rRNAs are methylated at G745. 23S rRNA of Gram-positives is methylated at G748. The position of an RNA methylation defines a sharp division between the Gram-negative and Gram-positive bacteria. Specificity of methylation is determined solely by the methyltransferase enzyme and is independent of the origin of the rRNA substrate. Pseudomonas putida shows intrinsic 23S rRNA methylation at G745. No methylation is determined with the recombinant protein in vivo or in vitro
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-
?
S-adenosyl-L-methionine + guanine745 in 23S rRNA
S-adenosyl-L-homocysteine + N1-methylguanine745 in 23S rRNA
Gram-negative 23 S rRNAs are methylated at G745. 23S rRNA of Gram-positives is methylated at G748. The position of an RNA methylation defines a sharp division between the Gram-negative and Gram-positive bacteria. Specificity of methylation is determined solely by the methyltransferase enzyme and is independent of the origin of the rRNA substrate. Pseudomonas stutzeri shows intrinsic 23S rRNA methylation at G745. No methylation is determined with the recombinant protein in vivo or in vitro
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?
S-adenosyl-L-methionine + guanine745 in 23S rRNA
S-adenosyl-L-homocysteine + N1-methylguanine745 in 23S rRNA
Gram-negative 23 S rRNAs are methylated at G745. 23S rRNA of Gram-positives is methylated at G748. The position of an RNA methylation defines a sharp division between the Gram-negative and Gram-positive bacteria. Specificity of methylation is determined solely by the methyltransferase enzyme and is independent of the origin of the rRNA substrate. Pseudomonas syringae shows intrinsic 23S rRNA methylation at G745. No methylation is determined with the recombinant protein in vivo or in vitro
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-
?
S-adenosyl-L-methionine + guanine745 in 23S rRNA
S-adenosyl-L-homocysteine + N1-methylguanine745 in 23S rRNA
-
Gram-negative 23 S rRNAs are methylated at G745. 23S rRNA of Gram-positives is methylated at G748. The position of an RNA methylation defines a sharp division between the Gram-negative and Gram-positive bacteria. Specificity of methylation is determined solely by the methyltransferase enzyme and is independent of the origin of the rRNA substrate
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?