1.4.1.8: valine dehydrogenase (NADP+)
This is an abbreviated version!
For detailed information about valine dehydrogenase (NADP+), go to the full flat file.
Reaction
Synonyms
dehydrogenase, valine (nicotinamide adenine dinucleotide phosphate), ValDH, valine dehydrogenase (nicotinamide adenine dinucleotide phosphate), VDH
ECTree
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Storage Stability
Storage Stability on EC 1.4.1.8 - valine dehydrogenase (NADP+)
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-20°C, 50 mM Tris-HCl buffer, pH 7.4, 5 mM 2-mercaptoethanol, 1 mM EDTA, 30% v/v glycerol, 24 h, 90% loss of activity
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-20°C, 70% (NH4)2SO4 suspension in 0.2 M Tris-HCl buffer, 5 months, stable
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-70°C, 25 mM sodium phosphate buffer pH 7, equal volume of glycerol, 3 months, stable
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-80°C, 100 mM Tris-HCl buffer, pH 7.4, 20% v/v glycerol, 2 months, stable
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-80°C, 50 mM potassium phosphate pH 7.5, 10% v/v glycerol, 1 mM EDTA, 5 mM 2-mercaptoethanol, 1 month, loss of specific activity
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4°C, 50 mM Tris-HCl buffer, pH 7.4, 5 mM 2-mercaptoethanol, 1 mM EDTA, 10% v/v glycerol, 48 h, stable
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