1.14.99.56: lytic cellulose monooxygenase (C4-dehydrogenating)
This is an abbreviated version!
For detailed information about lytic cellulose monooxygenase (C4-dehydrogenating), go to the full flat file.
Reaction
Synonyms
AA9, AA9A, Cel61a, chitin-binding domain 3 protein, FG02202.1, gh61-5, gh61e, GH61I, LPMO-02916, LPMO10B, LPMO9A, LPMO9C, LPMO9E, LPMO9f, LPMO9I, Micau_1630, NCU08760, Pte6, SCO0643, Tfu_1268
ECTree
1.14.99.56 lytic cellulose monooxygenase (C4-dehydrogenating)Advanced search results
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Substrates Products on EC 1.14.99.56 - lytic cellulose monooxygenase (C4-dehydrogenating)
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SUBSTRATE 
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
beta-(1->3,1->4)-glucan + ascorbate + O2
C4-oxidized glucan oligosaccharides + dehydroascorbate + H2O
substrate is regenerated amorphous cellulose
-
-
?
cellohexaose + acceptor + O2
oxidized cellobiose and cellotriose + reduced acceptor + H2O
-
-
-
-
?
cellohexaosyl-(2-aminobenzamide) + ascorbate + O2
cellotriose + oxidized cellotriosyl-(2-aminobenzamide) + dehydroascorbate + H2O
-
-
-
-
?
cellulose + ascorbate + O2
C4-oxidized gluco-oligosaccharides + dehydroascorbate + H2O
substrate is regenerated amorphous cellulose
sole release of C4-oxidized and non-oxidized gluco-oligosaccharides
-
?
cellulose + dopamine + O2
C4-oxidized gluco-oligosaccharides + 4-(2-aminoethyl)cyclohexa-3,5-diene-1,2-dione + H2O
-
dopamine shows 6% of the activity with ascorbate
-
?
cellulose acetate + ? + O2
? + H2O
-
lytic polysaccharide monooxygenase is able to cleave cellulose acetates with a degree of acetylation of up to 1.4. Preferentially, fragments with a low degree of acetylation are released
-
-
?
konjac glucomannan + ascorbic acid + O2
? + dehydroascorbate + H2O
-
-
-
-
?
phosphoric acid swollen cellulose + acceptor + O2
oxidized cellobiose and cellotriose + reduced acceptor + H2O
-
-
main products, C4 is the sole site of oxidation
-
?
phosphoric acid swollen cellulose + ascorbic acid + O2
? + dehydroascorbate + H2O
-
-
-
-
?
phosphoric acid-swollen cellulose + ascorbate + O2
cellooligosaccharide + dehydroascorbate + H2O
-
-
-
-
?
reduced xyloglucan oligosaccharide + ascorbic acid + O2
xyloglucan oligosaccharides + dehydroascorbic acid + H2O
pure xyloglucan oligosaccharide with DP14
-
-
?
soluble beta-glucan + ascorbic acid + O2
? + dehydroascorbic acid + H2O
-
-
-
-
?
tamarind xyloglucan + ascorbic acid + O2
? + dehydroascorbic acid + H2O
-
-
-
?
xyloglucan + ascorbate + O2
C4-oxidized oligosaccharides + dehydroascorbate + H2O
-
-
-
?
avicel PH 101 + ascorbic acid + O2
? + dehydroascorbic acid + H2O
-
-
-
?
avicel PH 101 + ascorbic acid + O2
? + dehydroascorbic acid + H2O
-
-
-
-
?
chitin + ascorbic acid + O2
? + dehydroascorbate + H2O
-
-
-
?
chitin + ascorbic acid + O2
? + dehydroascorbate + H2O
-
-
-
?
chitin + ascorbic acid + O2
? + dehydroascorbate + H2O
-
-
-
?
chitin + ascorbic acid + O2
? + dehydroascorbate + H2O
-
-
-
?
NaOH pretreated soy spent flakes + ascorbic acid + O2
? + dehydroascorbic acid + H2O
-
-
-
-
?
NaOH pretreated soy spent flakes + ascorbic acid + O2
? + dehydroascorbic acid + H2O
-
-
-
?
phosphoric acid swollen cellulase + ascorbic acid + O2
? + dehydroascorbate + H2O
-
-
-
?
phosphoric acid swollen cellulase + ascorbic acid + O2
? + dehydroascorbate + H2O
-
-
-
?
phosphoric acid swollen cellulose + ascorbate + O2
? + dehydroascorbate + H2O
-
the nonreducing end product is a 4-ketoaldose
-
?
phosphoric acid swollen cellulose + ascorbate + O2
? + dehydroascorbate + H2O
-
the nonreducing end product is a 4-ketoaldose
-
?
phosphoric acid swollen cellulose + ascorbic acid + O2
? + dehydroascorbic acid + H2O
-
-
-
-
?
phosphoric acid swollen cellulose + ascorbic acid + O2
? + dehydroascorbic acid + H2O
-
-
-
?
phosphoric acid swollen cellulose + ascorbic acid + O2
? + dehydroascorbic acid + H2O
-
-
-
?
phosphoric acid swollen cellulose + ascorbic acid + O2
? + dehydroascorbic acid + H2O
-
-
-
-
?
phosphoric acid swollen cellulose + ascorbic acid + O2
? + dehydroascorbic acid + H2O
-
-
-
?
phosphoric acid swollen cellulose + ascorbic acid + O2
? + dehydroascorbic acid + H2O
-
-
-
?
additional information
?
-
-
no substrate: xylan, starch, laminarin, chitin. cleavage of cleavage of hemicelluloses and phosphoric acid swollen cellulose C uses both C1- and C4-oxidizing mechanisms, reaction of EC 1.14.99.54 and EC 1.14.99.56
-
-
?
additional information
?
-
enzyme catalyzes mixed C1/C4 oxidative cleavage of cellulose, reactions of EC 1.14.99.54 and EC1.14.99.56, and xyloglucan, reaction of lytic xyloglucan monooxygenase, but is inactive toward other (1,4)-linked beta-glucans or chitin and cellooligosaccharides with a degree of polymerization DP 3-6. It shows broad specificity on xyloglucan, cleaving any glycosidic bond in the beta-glucan main chain, regardless of xylosyl substitutions. When incubated with a mixture of xyloglucan and cellulose, LPMO9A efficiently attacks the xyloglucan, whereas cellulose conversion is inhibited. no substrates: xyloglucan-heptamer, birchwood xylan, wheat arabinoxylan, konjac glucomannan, ivory nut mannan, beta-glucan from barley, lichenan from Icelandic moss, starch, and spruce galactoglucomannan
-
-
?
additional information
?
-
enzyme catalyzes mixed C1/C4 oxidative cleavage of cellulose, reactions of EC 1.14.99.54 and EC1.14.99.56, and xyloglucan, reaction of lytic xyloglucan monooxygenase, but is inactive toward other (1,4)-linked beta-glucans or chitin and cellooligosaccharides with a degree of polymerization DP 3-6. It shows broad specificity on xyloglucan, cleaving any glycosidic bond in the beta-glucan main chain, regardless of xylosyl substitutions. When incubated with a mixture of xyloglucan and cellulose, LPMO9A efficiently attacks the xyloglucan, whereas cellulose conversion is inhibited. no substrates: xyloglucan-heptamer, birchwood xylan, wheat arabinoxylan, konjac glucomannan, ivory nut mannan, beta-glucan from barley, lichenan from Icelandic moss, starch, and spruce galactoglucomannan
-
-
?
additional information
?
-
-
no substrate: locus bean glucomannan, tamarind xyloglucan, barley beta-1,3/1,4-glucan and birchwood xylan, carboxymethylcellulose or short cellooligosaccharides
-
-
?
additional information
?
-
the enzyme inserts oxygen at the 4-position. After oxygen insertion, the glycosidic bond is destabilized and likely broken by an elimination reaction, which may be catalyzed by the PMO or occur spontaneously. This elimination is irreversible because the carbon on the reducing or nonreducing end has been oxidized
-
-
?
additional information
?
-
the enzyme inserts oxygen at the 4-position. After oxygen insertion, the glycosidic bond is destabilized and likely broken by an elimination reaction, which may be catalyzed by the PMO or occur spontaneously. This elimination is irreversible because the carbon on the reducing or nonreducing end has been oxidized
-
-
?
additional information
?
-
enzyme cleaves cellulose, xyloglucan, reaction of lytic xyloglucan monooxogenase, mixed-linkage glucan and glucomannan. Oligosaccharides are cleaved using a C4-oxidizing mechanism, reaction of EC 1.14.99.56, whereas polysaccharides are cleaved with both C1- and C4-oxidizing mechanisms in varying proportions, reactions of EC 1.14.99.54 and EC 1.14.99.56
-
-
?
additional information
?
-
for isoforms LPMO9A, LPMO9B and LPMO9C, ascorbic acid is one of the best electron donors. Besides ascorbic acid, compounds bearing a 1,2-benzenediol moiety such as 3-methylcatechol, 3,4-dihydroxyphenylalanine, or a 1,2,3-benzenetriol moiety such as gallic acid, epigallocatechin-gallate give formation of oxidized and non-oxidized gluco-oligosaccharides. Sinapic acid actes as donor. No electron donor: quercetin or taxifolin, and tannic acid
-
-
?
additional information
?
-
no substrates: galactan, cellopentaose, or cellohexaose
-
-
?
