1.14.14.151: premnaspirodiene oxygenase
This is an abbreviated version!
For detailed information about premnaspirodiene oxygenase, go to the full flat file.
Word Map on EC 1.14.14.151
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1.14.14.151
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hyoscyamus
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muticus
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+-valencene
-
aroma
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environmentally
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regio
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sesquiterpenoid
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pichia
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grapefruit
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nootkatensis
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+-nootkatone
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pastoris
- 1.14.14.151
-
hyoscyamus
- muticus
-
+-valencene
- aroma
-
environmentally
-
regio
-
sesquiterpenoid
-
pichia
- grapefruit
- nootkatensis
-
+-nootkatone
- pastoris
Reaction
+ 2 [reduced NADPH-hemoprotein reductase] + 2 O2 = + 2 [oxidized NADPH-hemoprotein reductase] + 3 H2O
Synonyms
CYP71D55, EC 1.14.13.121, HPO, OsCYP71
ECTree
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KCat KM Value
KCat KM Value on EC 1.14.14.151 - premnaspirodiene oxygenase
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150
wild type enzyme, in 100 mM Tris-HCl, pH 7.5, at 30°C
610
(-)-vetispiradiene
mutant enzyme V482I, in 100 mM Tris-HCl, pH 7.5, at 30°C
1510
(-)-vetispiradiene
mutant enzyme V480I/A484I, in 100 mM Tris-HCl, pH 7.5, at 30°C
1590
(-)-vetispiradiene
mutant enzyme V482I/A484I, in 100 mM Tris-HCl, pH 7.5, at 30°C
60
wild type enzyme, product is 2beta-hydroxy-epiaristolochene, in 100 mM Tris-HCl, pH 7.5, at 30°C
80
5-epiaristolochene
mutant enzyme V482I, product is 2beta-hydroxy-epiaristolochene, in 100 mM Tris-HCl, pH 7.5, at 30°C
280
5-epiaristolochene
mutant enzyme V480I/A484I, product is 2beta-hydroxy-epiaristolochene, in 100 mM Tris-HCl, pH 7.5, at 30°C
650
5-epiaristolochene
mutant enzyme V482I/A484I, product is 2beta-hydroxy-epiaristolochene, in 100 mM Tris-HCl, pH 7.5, at 30°C
260
wild type enzyme, product is beta-nootkatol, in 100 mM Tris-HCl, pH 7.5, at 30°C
300
valencene
mutant enzyme V480I/A484I, product is beta-nootkatol, in 100 mM Tris-HCl, pH 7.5, at 30°C
740
valencene
mutant enzyme V482I, product is beta-nootkatol, in 100 mM Tris-HCl, pH 7.5, at 30°C
1310
valencene
mutant enzyme V482I/A484I, product is beta-nootkatol, in 100 mM Tris-HCl, pH 7.5, at 30°C