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0.03 - 0.45
D,L-isocitrate
0.002 - 0.385
DL-isocitrate
0.0183
homoisocitrate
-
6fold His-tagged enzyme, at 70°C in 50 mM N-2-hydroxyethylpiperadine-N0-2-ethanesulfonate-NaOH (pH 7.8), 0.2 M KCl, 0.2 mM MnCl2, 1mM NAD+
additional information
additional information
-
0.03
D,L-isocitrate
-
-
0.09
D-isocitrate
-
octameric wild-type enzyme with AMP, without DTT, pH 7.4, 24°C
0.1
D-isocitrate
-
octameric enzyme mutant IDH1/IDH2C150S with AMP, with or without DTT, pH 7.4, 24°C
0.1
D-isocitrate
-
octameric wild-type enzyme with AMP and DTT, pH 7.4, 24°C
0.17
D-isocitrate
-
octameric enzyme mutant IDH1/IDH2C56S/C150S/C242S with AMP, without DTT, pH 7.4, 24°C
0.19
D-isocitrate
-
octameric enzyme mutant IDH1/IDH2C56S/C150S/C242S with AMP and DTT, pH 7.4, 24°C
0.2
D-isocitrate
-
octameric enzyme mutant IDH1/IDH2C56S/C242S with AMP and DTT, pH 7.4, 24°C
0.32
D-isocitrate
-
octameric enzyme mutant IDH1/IDH2C56S/C242S with AMP, without DTT, pH 7.4, 24°C
0.48
D-isocitrate
-
octameric enzyme IDH1G15D/IDH2 with AMP and DTT, pH 7.4, 24°C
0.5
D-isocitrate
-
octameric enzyme IDH1G15D/IDH2 with AMP, without DTT, pH 7.4, 24°C
0.51
D-isocitrate
-
octameric wild-type enzyme without AMP, with DTT, pH 7.4, 24°C
0.53
D-isocitrate
-
octameric enzyme mutant IDH1/IDH2C150S without AMP and DTT, pH 7.4, 24°C
0.54
D-isocitrate
-
octameric enzyme mutant IDH1/IDH2C150S without AMP, with DTT, pH 7.4, 24°C
0.56
D-isocitrate
-
octameric wild-type enzyme without AMP and DTT, pH 7.4, 24°C
1.01
D-isocitrate
-
octameric enzyme mutant IDH1G15D/IDH2 without AMP and DTT, pH 7.4, 24°C
1.03
D-isocitrate
-
octameric enzyme mutant IDH1/IDH2C56S/C150S/C242S without AMP, with or without DTT, pH 7.4, 24°C
1.19
D-isocitrate
-
octameric enzyme IDH1G15D/IDH2 without AMP, with DTT, pH 7.4, 24°C
1.3
D-isocitrate
-
octameric enzyme mutant IDH1/IDH2C56S/C242S without AMP, with DTT, pH 7.4, 24°C
1.6
D-isocitrate
-
octameric enzyme mutant IDH1/IDH2C56S/C242S without AMP and DTT, pH 7.4, 24°C
0.002
DL-isocitrate
-
at pH 7.4 and 37°C
0.075
DL-isocitrate
-
pH 7.5, 37°C, recombinant wild-type enzyme
0.143
DL-isocitrate
-
pH 7.5, 37°C, recombinant enzyme mutant S102G
0.148
DL-isocitrate
-
pH 7.5, 37°C, recombinant enzyme mutant S102A
0.15
DL-isocitrate
-
pH 7.5, 37°C, recombinant enzyme mutant S102T
0.385
DL-isocitrate
-
pH 7.5, 37°C, recombinant enzyme mutant S102Y
0.009
isocitrate
-
at pH 8.5 and 42°C
0.0164
isocitrate
-
6fold His-tagged enzyme, at 70°C in 50 mM N-2-hydroxyethylpiperadine-N0-2-ethanesulfonate-NaOH (pH 7.8), 0.2 M KCl, 0.2 mM MnCl2, 1mM NAD+
0.031
isocitrate
-
mutant enzyme L580H/L591R/A640R, at pH 7.5 and 25°C
0.038
isocitrate
-
wild type enzyme, at pH 7.5 and 25°C
0.09
isocitrate
-
pH 7.2, 25°C, recombinant mutant betaY137E, in presence of 1 mM ADP
0.28
isocitrate
-
pH 7.2, 25°C, recombinant mutant betaY137F, in presence of 1 mM ADP
0.32
isocitrate
-
purified recombinant wild-type enzyme, pH 7.2, 25°C, in presence of 1 mM ADP
0.5
isocitrate
-
pH 7.2, 25°C, recombinant wild-type enzyme, in presence of 1 mM ADP
0.581
isocitrate
-
pH 7.4, 25°C
0.77
isocitrate
-
purified recombinant mutant beta-R99Q, pH 7.2, 25°C, in presence of 1 mM ADP
0.9
isocitrate
-
purified recombinant mutant beta-R99Q, pH 7.2, 25°C
0.92
isocitrate
-
pH 7.2, 25°C, recombinant mutant betaY137S, in presence of 1 mM ADP
1.2
isocitrate
-
pH 7.2, 25°C, recombinant mutant betaY137S
1.8
isocitrate
-
purified recombinant mutant gamma-R97Q, pH 7.2, 25°C, in presence of 1 mM ADP
1.8
isocitrate
-
pH 7.2, 25°C, recombinant mutant betaY137F
2
isocitrate
-
purified recombinant mutant gamma-R97Q, pH 7.2, 25°C
2
isocitrate
-
pH 7.2, 25°C, recombinant wild-type enzyme
2.2
isocitrate
-
purified recombinant wild-type enzyme, pH 7.2, 25°C
2.2
isocitrate
-
wild type enzyme in 20 mM isocitrate and 1mM MnSO4
2.2
isocitrate
-
25°C, pH 7.2, wild-type enzyme
2.4
isocitrate
-
pH 7.2, wild-type enzyme
2.9
isocitrate
-
pH 7.2, beta-subunit mutant enzyme D192N
3.4
isocitrate
-
D234C, alpha-subunit mutant, in 20 mM isocitrate and 1mM MnSO4
3.4
isocitrate
-
25°C, pH 7.2, alpha-subunit mutant enzyme D234C
3.4
isocitrate
-
pH 7.2, 25°C, recombinant mutant betaY137E
3.5
isocitrate
-
D206N, alpha-subunit mutant, in 20 mM isocitrate and 1mM MnSO4
3.5
isocitrate
-
25°C, pH 7.2, alpha-subunit mutant enzyme D206N
5.1
isocitrate
-
D217N, beta-subunit mutant, in 20 mM isocitrate and 1mM MnSO4
5.1
isocitrate
-
25°C, pH 7.2, beta-subunit mutant enzyme D217N
9.1
isocitrate
-
pH 7.2, alpha-subunit mutant enzyme D181N
13
isocitrate
-
pH 7.2, gamma-subunit mutant enzyme D190N
20
isocitrate
-
D230C, alpha-subunit mutant, in 20 mM isocitrate and 1mM MnSO4
20
isocitrate
-
25°C, pH 7.2, alpha-subunit mutant enzyme D230C
41
isocitrate
-
D215N, gamma-subunit mutant, in 20 mM isocitrate and 1mM MnSO4
41
isocitrate
-
25°C, pH 7.2, gamma-subunit mutant enzyme D215N
0.22
Mn2+
-
purified recombinant wild-type enzyme and mutant beta-R99Q, pH 7.2, 25°C
0.39
Mn2+
-
purified recombinant mutant gamma-R97Q, pH 7.2, 25°C
0.004
NAD+
-
purified recombinant mutant gamma-R97Q, pH 7.2, 25°C
0.014
NAD+
-
purified recombinant mutant beta-R99Q, pH 7.2, 25°C
0.0289
NAD+
pH 75, 25°C, recombinant His6-tagged enzyme
0.04
NAD+
-
25°C, pH 7.2, alpha-subunit mutant enzyme D234C
0.05
NAD+
-
at pH 7.4 and 37°C
0.06
NAD+
-
pH 7.2, 25°C, recombinant wild-type enzyme
0.066
NAD+
pH 8.0, 70°C, mutant enzyme D328K
0.068
NAD+
pH 8.0, 70°C, wild-type enzyme
0.0683
NAD+
pH 8.0, 70°C, the Km-value for NADP+ is about 65fold higher than the KM-value for NAD+
0.07
NAD+
-
25°C, pH 7.2, wild-type enzyme
0.073
NAD+
-
purified recombinant wild-type enzyme, pH 7.2, 25°C
0.0742
NAD+
pH 75, 25°C, recombinant His6-tagged enzyme
0.0771
NAD+
-
6fold His-tagged enzyme, at 70°C in 50 mM N-2-hydroxyethylpiperadine-N0-2-ethanesulfonate-NaOH (pH 7.8), 0.2 M KCl, 0.2 mM MnCl2
0.08
NAD+
-
pH 7.2, wild-type enzyme
0.088
NAD+
-
wild type enzyme, at pH 7.5 and 25°C
0.09
NAD+
-
pH 7.2, 25°C, recombinant mutant betaY137E
0.099
NAD+
-
7-fold mutant
0.113
NAD+
-
at pH 8.5 and 42°C
0.12
NAD+
-
pH 7.2, 25°C, recombinant mutant betaY137F
0.121
NAD+
-
wild type enzyme, at pH 8.0 and 25°C
0.126
NAD+
pH 75, 25°C, recombinant His6-tagged enzyme
0.136
NAD+
-
pH 7.4, 25°C
0.136
NAD+
pH 75, 25°C, recombinant His6-tagged enzyme
0.14
NAD+
-
25°C, pH 7.2, alpha-subunit mutant enzyme D206N
0.154
NAD+
-
pH 7.5, 37°C, recombinant enzyme
0.18
NAD+
pH 9.0, 30°C, native enzyme
0.19
NAD+
-
25°C, pH 7.2, gamma-subunit mutant enzyme D215N
0.226
NAD+
-
pH 7.6, temperature not specified in the publication, His6-tagged recombinant wild-type enzyme, with Mn2+
0.245
NAD+
-
pH 7.5, 37°C, with Mn2+, recombinant enzyme
0.246
NAD+
-
pH 7.5, 37°C, recombinant enzyme mutant S102A
0.263
NAD+
wild-type enzyme, with Mg2+, pH 7.5, 35°C
0.265
NAD+
-
pH 7.6, temperature not specified in the publication, His6-tagged recombinant wild-type enzyme, with Mg2+
0.295
NAD+
-
pH 7.5, 37°C, recombinant enzyme mutant S102G
0.309
NAD+
wild-type enzyme, with Mn2+, pH 7.5, 35°C
0.312
NAD+
-
pH 7.5, 37°C, with Mg2+, recombinant enzyme
0.33
NAD+
-
membrane-associated ICDH
0.336
NAD+
pH 8.0, 70°C, mutant enzyme D328K/I329Y
0.35
NAD+
-
pH 7.5, 37°C, recombinant enzyme mutant S102T
0.384
NAD+
-
mutant enzyme D268K, at pH 8.0 and 25°C
0.493
NAD+
pH 75, 25°C, recombinant His6-tagged mutant L584H/D595R
0.63
NAD+
-
pH 7.2, 25°C, recombinant mutant betaY137S
0.664
NAD+
mutant D487R/L488H, with Mn2+, pH 7.5, 35°C
0.68
NAD+
-
25°C, pH 7.2, beta-subunit mutant enzyme D217N
0.71
NAD+
-
pH 7.2, beta-subunit mutant enzyme D192N
0.738
NAD+
-
pH 7.6, temperature not specified in the publication, His6-tagged recombinant mutant D344R, with Mn2+
0.903
NAD+
-
in the presence of Mg2+, at pH 8.0 and 25°C
1.1
NAD+
-
25°C, pH 7.2, alpha-subunit mutant enzyme D230C
1.1325
NAD+
-
in the presence of Mn2+, at pH 8.0 and 25°C
1.2
NAD+
-
pH 7.2, alpha-subunit mutant enzyme D181N
1.5
NAD+
-
pH 7.2, gamma-subunit mutant enzyme D190N
1.56
NAD+
-
pH 7.5, 37°C, recombinant enzyme mutant S102Y
2.948
NAD+
pH 75, 25°C, recombinant His6-tagged mutant D326R/M327H
3.15
NAD+
-
mutant enzymeD268K/I269Y/A275V, at pH 8.0 and 25°C
4.413
NAD+
-
mutant enzyme D268K/I269Y, at pH 8.0 and 25°C
4.5
NAD+
-
mutant enzyme L580H/L591R/A640R, at pH 7.5 and 25°C
20.65
NAD+
-
pH 7.6, temperature not specified in the publication, His6-tagged recombinant mutant D344R/M345H, with Mn2+
0.0082
NADP+
pH 75, 25°C, recombinant His6-tagged mutant D326R/M327H
0.0114
NADP+
pH 75, 25°C, recombinant His6-tagged mutant L584H/D595R
0.0139
NADP+
-
pH 7.6, temperature not specified in the publication, His6-tagged recombinant mutant D344R/M345H, with Mn2+
0.031
NADP+
-
mutant enzymeD268K/I269Y/A275V, at pH 8.0 and 25°C
0.067
NADP+
-
pH 7.6, temperature not specified in the publication, His6-tagged recombinant mutant D344R, with Mn2+
0.087
NADP+
-
mutant enzyme D268K/I269Y, at pH 8.0 and 25°C
0.101
NADP+
-
mutant enzyme D268K, at pH 8.0 and 25°C
0.157
NADP+
mutant D487R/L488H, with Mn2+, pH 7.5, 35°C
0.162
NADP+
pH 8.0, 70°C, mutant enzyme D328K
0.207
NADP+
-
mutant enzyme L580H/L591R/A640R, at pH 7.5 and 25°C
0.211
NADP+
pH 8.0, 70°C, mutant enzyme D328K/I329Y
0.476
NADP+
pH 75, 25°C, recombinant His6-tagged enzyme
0.513
NADP+
pH 75, 25°C, recombinant His6-tagged enzyme
1.827
NADP+
pH 75, 25°C, recombinant His6-tagged enzyme
2.211
NADP+
pH 75, 25°C, recombinant His6-tagged enzyme
2.339
NADP+
-
wild type enzyme, at pH 8.0 and 25°C
3.354
NADP+
-
pH 7.6, temperature not specified in the publication, His6-tagged recombinant wild-type enzyme, with Mg2+
4.3
NADP+
-
wild type enzyme, at pH 7.5 and 25°C
4.314
NADP+
-
pH 7.6, temperature not specified in the publication, His6-tagged recombinant wild-type enzyme, with Mn2+
4.44
NADP+
pH 8.0, 70°C, wild-type enzyme
5.8
NADP+
-
7-fold mutant
6.57
NADP+
pH 9.0, 30°C, native enzyme
7.7
NADP+
-
pH 7.5, 37°C, with Mn2+, recombinant enzyme
8.2
NADP+
-
pH 7.5, 37°C, with Mg2+, recombinant enzyme
additional information
additional information
kinetic properties of the native and the recombinant enzyme are similar
-
additional information
additional information
-
kinetic properties of the native and the recombinant enzyme are similar
-
additional information
additional information
-
kinetics and ligand binding
-
additional information
additional information
-
kinetics and ligand binding
-
additional information
additional information
-
NAD+-specific D-isocitrate dehydrogenase is an allosterically regulated. AMP and NAD+ binding kinetics of enzyme mutants in presence or absence of DTT, overview
-
additional information
additional information
-
yeast NAD+-specific isocitrate dehydrogenase is an allosterically regulated
-
additional information
additional information
-
OtIDH exhibits a sigmoidal kinetic behavior in response to isocitrate unlike other homodimeric enzyme homologues, and a remarkably high affinity for isocitrate unlike other heterooligomeric enzyme homologues
-