EC Number |
Protein Variants |
Reference |
---|
2.1.1.B109 | D466A |
site-directed mutagenesis, inactive mutant |
-, 721028 |
2.1.1.B109 | D547A |
site-directed mutagenesis, the mutant shows reduced activity compared to the wild-type |
-, 721028 |
2.1.1.B109 | F481A |
site-directed mutagenesis, the mutant shows unaltered activity compared to the wild-type |
-, 721028 |
2.1.1.B109 | K559A |
site-directed mutagenesis, the mutant shows unaltered activity compared to the wild-type |
-, 721028 |
2.1.1.B109 | more |
deletion of the conserved region near the C-terminus, i.e. delD602-Q621 and construction of the abp140DELTA knockout strain, expression of delD602-Q621 in the null mutant strain. Complementation of the yor239wD strain with plasmid-encoded ABP140 containing various mutations, in vitro reconstitution of m3C32 by recombinant Trm140p |
-, 721028 |
2.1.1.B109 | more |
short-hairpin(sh)RNA knockdown of METTL2B mRNA in HeLa cells |
721028 |
2.1.1.B109 | Y569A |
site-directed mutagenesis, the mutant shows unaltered activity compared to the wild-type |
721028 |