EC Number   |
Protein Variants |
Reference |
|---|
   2.1.1.72 | C54G |
the mutant has wild type activity |
720816 |
| 2.1.1.72 | D181A |
site-directed mutagenesis, inactive mutant, mutation abolishes base flipping, D181 seems to contact and stabilize the flipped base, i.e. the intermediate state of the base flipping process |
659715 |
| 2.1.1.72 | D194A |
the Hia5 mutant shows loss of DNA MTase activity |
-, 720595 |
| 2.1.1.72 | D347A |
the mutant shows wild type-like methylation activity |
733427, 757760 |
| 2.1.1.72 | D71A |
phenotype of native Dam |
-, 673989 |
| 2.1.1.72 | E200G |
produces wild-type phenotype |
676206 |
| 2.1.1.72 | E76A |
phenotype of native Dam |
-, 673989 |
| 2.1.1.72 | F195S |
the mutant is not able to bind to the S-adenosyl-L-methionine as effectively as the wild type enzyme and is catalytically inactive |
719450 |
| 2.1.1.72 | H171A |
the mutant is almost as active as the wild type enzyme |
702115 |
| 2.1.1.72 | H317A |
the mutant shows a severely decreased methylation activity (to less than 10%) compared to the wild type enzyme |
757760 |
