1.3.3.5 | hanging drop vapour diffusion method, 0.003 ml of 9.2 mg/ml protein in 10 mM Tris-HCl, pH 8.0, are mixed with 0.003 ml of recipitant solution containing 10% 2-methyl-2,4-pentanediol, 1.44 M ammonium sulfate, 10% glycerol and 0.5 M KCl, equilibration against 0.2-1.0 ml precipitant solution at 20°C, X-ray diffraction structure determination and analysis at 2.3-2.5 A resolution, modelling |