1.2.1.47 | purified human ALDH9A1 in apoform and in complex with NAD+ or 4-(trimethylamino)butyraldehyde, hanging drop vapour diffusion method, mixing of 28 mg/ml protein in 50 mM Tris/HCl, pH 7.5, and 150 mM NaCl, with 50 mM NAD+ and an equal volume of a precipitant solution containing 12% w/v PEG 4000, 0.1 M sodium citrate, pH 5.6, and 2.5% isopropanol, X-ray diffraction structure determination and analysis at 2.3 A, 2.9 A, and 2.5 A resolution, molecular replacement using the structure of BADH from Gadus morhua subsp. callarias liver as a search model (PDB IDs 1BPW and 1A4S), structure modeling |
1.2.1.47 | purified recombinant detagged enzyme in complex with NAD+ and inhibitor diethylaminobenzaldehyde, hanging drop vapour diffusion method, mixing of 6 mg/ml protein in 50 mM Tris-HCl, pH 8.0, 600 mM NaCl, 5% glycerol, 0.5 mM TCE, 5 mM DEAB, and 10 mM NAD+, with reservoir solution containing 0.1 M NaCl, 0.05 M Bis-Tris, pH 6.5, 0.1 M ammonium acetate, 0.05 M HEPES, pH 7.5, and 25% w/v PEG 3350, method optimization, X-ray diffraction structure determination and analysis at 2.50-2.64 A resolution, molecular replacement using structures of cod liver betaine ALDH (PDB ID 1A4S) and apo-ALDH9A1 (PDB ID 6QAP) as search models, modeling |