EC Number |
Temperature Stability Minimum [°C] |
Temperature Stability Maximum [°C] |
Reference |
---|
1.14.15.4 | 2 |
- |
14% residual activity of 11beta- and 18-hydroxylation |
285390 |
1.14.15.4 | 2 |
- |
crude extract, 7 mg/ml, 24 h, loss of half the activity, DEAE fraction unstable |
285388 |
1.14.15.4 | 30 |
- |
30 min, 30 mM sodium phosphate buffer, pH 8.0, decrease to 30%, improved to 45-70% by addition of 1 mM GSH, 0.2 mM 11-deoxycortisol, 0.5 mM PMSF or 20% glycerol |
285409 |
1.14.15.4 | 30 |
- |
after preincubation for 20 min, 27% residual activity, after 60 min 5.7% |
285390 |
1.14.15.4 | 30 |
- |
in presence of phospholipids, catalytic activity stable to extended incubation and increased resistance of hemoprotein to denaturation |
285402 |
1.14.15.4 | 30 |
- |
purified enzyme, loss of 75% activity after 3 h, and of all activity after 8 h |
686458 |
1.14.15.4 | 50 |
- |
complete inactivation |
285390 |