EC Number   |
|---|
   2.3.1.B43 | - |
| 2.3.1.B43 | crystals are grown by hanging drop vapor diffusion method, truncated SIRT5(34302) is cloned and expressed in Escherichia coli |
| 2.3.1.B43 | expressed in Escherichia coli BL21(DE3) cells |
| 2.3.1.B43 | expression in Escherichia coli |
| 2.3.1.B43 | gene SIRT5 |
| 2.3.1.B43 | gene SIRT5, alternative splicing of the human SIRT5 mRNA results in two distinct SIRT5 protein isoforms, differing at their C-termini, recombinant expression of His-tagged wild-type and mutant enzymes in Escherichia coli strain BL21(DE3, method, overview) |
| 2.3.1.B43 | gene SIRT5, genotyping of the Ctl and OE mice. Sirt5 hepatic overexpressing (ob/ob-SIRT5 OE) mouse containing at least one allele of alternative Sirt5 gene and Alb-cre. The other one is control ob/ob mouse (ob/ob- control) containing only the floxed Sirt5 KI allele |
| 2.3.1.B43 | gene SIRT5, quantitative real-time PCR expression analysis |
| 2.3.1.B43 | gene SIRT5, recombinant expression of His-tagged enzyme in Escherichia coli strains Rosetta2(DE3) and BL21(DE3) |
| 2.3.1.B43 | gene SIRT5, recombinant expression of N-terminally His-tagged enzyme in Escherichia coli strain BL21(DE3) |
