EC Number   |
|---|
   2.1.1.214 | gene Tk0981 |
| 2.1.1.214 | gene TRM112, phylogenetic analysis, expression of His-tagged Trm112p, alone or with Trm9p, in Escherichia coli strain S15 (DE3-pLysS), Trm112p forms a complex with Trm9p, which renders the latter soluble |
| 2.1.1.214 | gene YOL124c, recombinant expression of soluble C-terminally His6-tagged wild-type and mutant enzymes in Escherichia coli strain BL21-Gold (DE3), coexpression of yeast Trm11 with yeast Trm112 in Escherichia coli, the holonezyme complex is active |
| 2.1.1.214 | recombinant Trm11p expressed in Escherichia coli exhibited no m2G10 formation activity. Trm112p and Trm11p are both required for the formation of m2G10 in vivo as well as in vitro |
