EC Number |
Substrates |
Organism |
Products |
Reversibility |
---|
1.1.1.4 | 2,3-pentanedione + NADH + H+ |
small amounts of 2,3-pentanediol are produced as by-product |
Alkalihalobacillus clausii DSM 8716T |
3-hydroxy-2-pentanone + NAD+ |
- |
r |
1.1.1.4 | 5-methyl-2,3-hexanedione + NADH + H+ |
small amounts of 5-methyl-2-hydroxy-3-hexanone and traces of (3S)-5-methyl-3-hydroxy-2-hexanone are produced as by-products |
Alkalihalobacillus clausii DSM 8716T |
(3R)-5-methyl-3-hydroxy-2-hexanone + NAD+ |
- |
r |
1.1.1.4 | more |
substrate specificity analysis, overview. Enzyme BcBDH catalyzes the selective asymmetric reduction of prochiral 1,2-diketones to the corresponding HK and, in some cases, the reduction of the same to the corresponding 1,2-diols. Aliphatic diketones, like 2,3-pentanedione, 2,3-hexanedione, 5-methyl-2,3-hexanedione, 3,4-hexanedione, and 2,3-heptanedione are well transformed. In addition, surprisingly alkyl phenyl dicarbonyls, like 2-hydroxy-1-phenylpropan-1-one and phenylglyoxal are accepted, whereas their derivatives with two phenyl groups are not substrates. The biocatalytic reduction of 5-methyl-2,3-hexanedione to mainly 5-methyl-3-hydroxy-2-hexanone with only small amounts of 5-methyl-2-hydroxy-3-hexanone within an enzyme membrane reactor is demonstrated. Stereoselectivity and conversion is analyzed by carrying out the reduction reaction of selected diketones and alpha-hydroxyketones |
Alkalihalobacillus clausii DSM 8716T |
? |
- |
- |
1.1.1.4 | (R,R)-butane-2,3-diol + NAD+ |
- |
Bacillus amyloliquefaciens |
(R)-acetoin + NADH + H+ |
- |
r |
1.1.1.4 | (R,R)-butane-2,3-diol + NAD+ |
- |
Bacillus amyloliquefaciens UCMB5033 |
(R)-acetoin + NADH + H+ |
- |
r |
1.1.1.4 | (2R,3R)-butane-2,3-diol + NAD+ |
- |
Bacillus licheniformis |
(R)-acetoin + NADH + H+ |
- |
r |
1.1.1.4 | (2S,3S)-butane-2,3-diol + NAD+ |
very low activity |
Bacillus licheniformis |
(2S)-acetoin + NADH + H+ |
- |
? |
1.1.1.4 | (R)-acetoin + NADH + H+ |
- |
Bacillus licheniformis |
(2R,3R)-butane-2,3-diol + NAD+ |
- |
r |
1.1.1.4 | (R)-acetoin + NADH + H+ |
- |
Bacillus licheniformis |
(R,R)-butane-2,3-diol + NAD+ |
- |
r |
1.1.1.4 | (R,R)-butane-2,3-diol + NAD+ |
- |
Bacillus licheniformis |
(R)-acetoin + NADH + H+ |
- |
r |