EC Number |
kcat/KM Value [1/mMs-1] |
kcat/KM Value Maximum [1/mMs-1] |
Substrate |
Reference |
---|
1.1.1.87 | 0.022 |
- |
D-malate |
wild type enzyme, with NAD+ as cosubstrate, at pH 8.0 and 60°C |
760522 |
1.1.1.87 | 0.11 |
- |
NAD+ |
wild type enzyme, with D-malate as cosubstrate, at pH 8.0 and 60°C |
760522 |
1.1.1.87 | 0.35 |
- |
NAD+ |
recombinant His-tagged enzyme, pH 7.8, 20°C |
722303 |
1.1.1.87 | 0.44 |
- |
isocitrate |
mutant enzyme T71V, with NAD+ as cosubstrate, at pH 8.0 and 60°C |
760522 |
1.1.1.87 | 0.44 |
- |
NAD+ |
native enzyme, pH 7.8, 20°C |
722303 |
1.1.1.87 | 0.45 |
- |
isocitrate |
mutant enzyme I82N, with NAD+ as cosubstrate, at pH 8.0 and 60°C |
760522 |
1.1.1.87 | 0.56 |
- |
NAD+ |
mutant enzyme I82N, with isocitrate as cosubstrate, at pH 8.0 and 60°C |
760522 |
1.1.1.87 | 0.88 |
- |
NAD+ |
mutant enzyme T71V, with isocitrate as cosubstrate, at pH 8.0 and 60°C |
760522 |
1.1.1.87 | 0.92 |
- |
isocitrate |
mutant enzyme L81P, with NAD+ as cosubstrate, at pH 8.0 and 60°C |
760522 |
1.1.1.87 | 0.93 |
- |
NAD+ |
mutant enzyme S80A, with isocitrate as cosubstrate, at pH 8.0 and 60°C |
760522 |