EC Number   |
General Information |
Reference |
|---|
  2.1.1.197 | malfunction |
the DELTAbioC deletion mutant is only able to grow in biotin supplemented medium. Also supplementation with pimelic acid, and putative intermediates in the pathway, the enoyl, 3-keto and 3-hydroxy derivatives of the monomethyl ester of glutarate and the 3-keto and 3-hydroxy derivatives of the monomethyl ester of pimelate, allow growth of the mutant strain in the absence of biotin, but the 2-keto, 2-hydroxy and 4-keto derivatives, as well as monomethyl esters of C4, C6, C8, C9 and C11 dicarboxylates, do not, overview |
710038 |
| 2.1.1.197 | metabolism |
the pimeloyl moiety of biotin is synthesized by a modified fatty acid synthetic pathway in which the omega-carboxyl group of a malonyl-thioester is methylated by BioC which allows recognition of this atypical substrate by the fatty acid synthetic enzymes. The malonyl-thioester methyl ester enters fatty acid synthesis as the primer and undergoes two reiterations of the fatty acid elongation cycle to give pimeloyl-acyl carrier protein methyl ester which is hydrolyzed to pimeloyl-ACP and methanol by BioH |
710038 |
| 2.1.1.197 | physiological function |
deletion and complementation analysis of the biotin gene cluster. Mutants in BioC are blocked early in the biosynthetic pathway and complement mutants in bioA, bioB, and bioD |
708906 |
| 2.1.1.197 | physiological function |
in biotin synthesis, the pimeloyl moiety is synthesized by a modified fatty acid synthetic pathway in which the omega-carboxyl group of a malonyl-thioester is methylated by BioC, which allows recognition of this atypical substrate by the fatty acid synthetic enzymes. The malonyl-thioester methyl ester enters fatty acid synthesis as the primer and undergoes two reiterations of the fatty acid elongation cycle to give pimeloyl-acyl carrier protein methyl ester. Supplementation of biotin-free medium with any of malonic, glutaric and pimelic acid monomethyl ester allows for acyl-ACP-synthetase-dependent growth of the bioC deletion strain |
710038 |
| 2.1.1.197 | physiological function |
the role of BioC is to convert the free carboxyl group of a malonylthioester to its methyl ester by transfer of a methyl group from SAM. Methylation both cancels the charge of the carboxyl group and provides a methyl carbon to mimic the methyl ends of normal fatty acyl chains |
710038 |
