EC Number   |
|---|
    2.7.1.30 | - |
| 2.7.1.30 | application of triazine dye affinity chromatography to large-scale purification |
| 2.7.1.30 | GSTrap FF column chromatography, gel filtration |
| 2.7.1.30 | HiTrapQ HP column, Bio-Scale CHT20-I column, all purification procedures are carried out at 277 K. |
| 2.7.1.30 | metal-chelate affinity chromatography using a Ni-NTA column, anion exchange chromatography using a Mono Q 5/50 GL column and gel filtration chromatography using s Superdex 200 10/30 GL column,all purification steps are performed in standard buffer (20 mM TrisHCl (pH 7.5), 10 mM glycerol, 1 mM beta-mercaptoethanol) at 4°C excluding the affinity chromatography purification |
| 2.7.1.30 | mutant enzyme G230D |
| 2.7.1.30 | native and mutant enzyme |
| 2.7.1.30 | Ni-NTA affinity resin column chromatography and gel filtration |
| 2.7.1.30 | Ni-NTA agarose column chromatography and Superdex 200 gel filtration |
| 2.7.1.30 | nickel affinity column chromatography |
