EC Number |
---|
2.1.1.148 | - |
2.1.1.148 | by nickel chromatography and gel filtration, more than 95% pure |
2.1.1.148 | from transformed E. coli |
2.1.1.148 | from transformed E. coli using His-tag |
2.1.1.148 | nickel affinity and ion-exchange chromatography |
2.1.1.148 | nickel chelating column chromatography, GSH-Sepharose column chromatography, and Superdex 200 gel filtration |
2.1.1.148 | on Ni-NTA column |
2.1.1.148 | to near homogeneity, by heat treatment at 85°C for 15 min, followed by Ni2+ column chromatography |