EC Number |
---|
1.2.1.105 | - |
1.2.1.105 | 2-oxoglutarate dehydrogenase complex |
1.2.1.105 | 2-oxoglutarate dehydrogenase complex from ox heart, by PEG fractionation, ultracentrifugation, and gel filtration |
1.2.1.105 | by gel filtration |
1.2.1.105 | dissociation of the 2-oxoglutarate dehydrogenase complex into two fractions on a calcium phosphate gel-cellulose column in the presence of 2.5 M urea and 1% ammonium sulfate releases lipoamide dehydrogenase. The second fraction precipitates with between 0.23 and 0.40 saturation of ammonium sulfate and exhibits 2-oxoglutarate dehydrogenase and lipoate succinyltransferase activities. All three of the isolated enzymes are required to catalyze a CoA and NAD-linked oxidation of 2-oxoglutarate |
1.2.1.105 | partially purified from skeletal muscle of euthermic and hibernating ground squirrels |
1.2.1.105 | purification procedure for the 2-oxoglutarate dehydrogenase and the pyruvate dehydrogenase complexes from mitochondria. After fractionated precipitations with polyethylene glycol, elimination of thiol proteins, and gel-filtration chromatography, the resulting preparations contain both activities. Covalent chromatography on thiol-activated Sepharose CL-4B allows the specific binding of the 2-oxoglutarate dehydrogenase complex activity in the presence of 2-oxoglutarate, the pyruvate dehydrogenase complex activity is retained in the presence of pyruvate |