EC Number   |
|---|
    1.11.1.11 | - |
| 1.11.1.11 | ammonium sulfate precipitation, butyl-Toyopearl column chromatography, DEAE-cellulofine column chromatography, and Sephadex G-75 gel filtration |
| 1.11.1.11 | Arabidopsis thaliana leaf extracts are prepared |
| 1.11.1.11 | both APX 1 and APX 2 |
| 1.11.1.11 | Escherichia coli recombinant stromal ascorbate peroxidase is purified with a HisTrap kit, proteins are used to immunize rabbits and rats |
| 1.11.1.11 | mitochondria are prepared and fractionated |
| 1.11.1.11 | native enzyme 152fold from leaf juice by ultrafiltration, dialysis, and anion exchange chromatography, to homogeneity |
| 1.11.1.11 | of recombinant enzyme 1: using column chromatography on DEAE Bio-Gel A agarose and column chromatography on Sephacryl S-300, of recombinant enzyme 2: using column chromatography on DEAE Bio-Gel A agarose, ammonium sulfate fractionation, dialysis, chromatofocusing on a polybuffer 94 exchanger and column chromatography on Sephacryl S-300 |
| 1.11.1.11 | of the native enzyme, using ammonium sulfate precipitation, column chromatography on Phenyl-Sepharose, second ammonium sulfate precipitation, and column chromatography on Superdex 200 and Q Sepharose |
| 1.11.1.11 | of the recombinant enzyme, using affinity chromatography on amylose colum, and column chromatography on hydroxylapatite and FFQ-Sepharose |
