  2.4.1.333 | synthesis |
synthesis of beta-1,2-glucan with sophorose as an acceptor through three synthesis steps using sucrose phosphorylase and 1,2-beta-oligoglucan phosphorylase. The first step is performed in 200 microl reaction solution containing 5 mM sophorose and 1.0 M sucrose. beta-1,2-Glucan in a part of the resultant solution is hydrolyzed to beta-1,2-glucooligosaccharides by beta-1,2-glucanase. The second synthesis is performed in 25 times the volume for the first synthesis. The hydrolysate solution (1% volume of the reaction solution) is used as an acceptor. After treatment with the beta-1,2-glucanase again, the third synthesis is performed in 200 times the volume of the second synthesis (1 l). More than 140 g of beta-1,2-glucan is synthesized using approximately 20 microg of sophorose as the starting acceptor material |
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