EC Number   |
Application |
Reference |
|---|
   1.5.1.36 | analysis |
amperometric NADH biosensor system that employs the allosterically modulated reductase component of the 4-hydroxyphenylacetate hydroxylase in an adapted osmium(III)-complex-modified redox polymer film for analyte quantification. The activity of the reductase is enhanced upon binding of effector 4-hydroxy-phenylacetic acid, allowing the acceleration of the substrate conversion rate on the sensor surface by in situ addition or preincubation with 4-hydroxy-phenylacetic acid. Acceleration of NADH oxidation amplifies the response of the biosensor, with a 1.5fold increase in the sensitivity of analyte detection |
763881 |
| 1.5.1.36 | synthesis |
generation of FMNH2 by enzyme encapsulated in poly(lactide-co-glycolide) nanoparticles. Enzymatic activity, stability, and reusability of the nanoparticles prepared using three different methods [(o/w), water in oil in water (w/o/w), and solid in oil in water (s/o/w)] are compared. The solid in oil in water method provides the optimal conditions for encapsulation of the reudctase, giving the highest enzyme activity, stability, and reusability. The solid in oil in water method improves enzyme activity about 11-and 9fold compared to water in oil in water and oil in water methods. Solid in oil in water nanoparticles can be reused 14 times with nearly 50% activity remaining |
764823 |
| 1.5.1.36 | synthesis |
in vivo production of ortho-hydroxylated flavonoids by recombinant Escherichia coli. When HpaC is linked with an S-Tag on the C terminus, the enzyme activity is significantly affected. The optimal culture conditions are a substrate concentration of 80 mg/l, an induction temperature of 28°C, an M9 medium, and a substrate delay time of 6 h after IPTG induction. The efficiency of eriodictyol conversion from recombinant strains fed naringin is up to 57.67. Highest conversion efficiencies for production of catechin and caffeate are 35.2 % and 32.93%, respectively |
-, 765429 |
| 1.5.1.36 | synthesis |
overexpression of the HpaB and HpaC genes in Saccharomyces cerevisiae achieves hydroxytyrosol titers of 1.15 mg/l and 4.6 mg/l in a minimal medium in which either 1 mM tyrosine or 1 mM tyrosol are respectively added |
-, 764691 |
