EC Number   |
Natural Substrates |
|---|
   2.1.1.309 | more |
Trm112 interacts directly with Bud23 in vitro and that it is required for Bud23 stability in vivo |
| 2.1.1.309 | more |
BUD23 physically interacts with middle-stage 40S biogenesis factors |
| 2.1.1.309 | more |
Bud23-Trm112 interacts directly with the box C/D snoRNA U3-associated DEAH RNA helicase Dhr1 supposedly involved in central pseudoknot formation. Bud23Trm112 might also contribute to controlling formation of this irreversible and dramatic structural reorganization essential to overall folding of small subunit rRNA |
| 2.1.1.309 | more |
secondary structure of the 18S rRNA, overview |
| 2.1.1.309 | S-adenosyl-L-methionine + guanine10 in tRNA |
conserved methylation of G1575 of 18S rRNA, in the P-site of the small subunit of the ribosome, Bud23 methylates G1575 in 18S rRNA in a helix that stacks coaxially with the central pseudoknot. Bud23 protein, but not its methyltransferase activity, is important for its function |
| 2.1.1.309 | S-adenosyl-L-methionine + guanine10 in tRNA |
m7G1639 methylation |
| 2.1.1.309 | S-adenosyl-L-methionine + guanine1575 in 18S rRNA |
- |
| 2.1.1.309 | S-adenosyl-L-methionine + guanine1575 in 18S rRNA |
Bud23 is active in 18S rRNA N7G methylation as a heterodimer in a complex with Trm112. Trm112 is a small zinc finger protein that interacts with and activates three class I MTases in addition to Bud23. N7-methylguanosine introduced at position 1575 on 18S rRNA by Bud23-Trm112 is at a ridge forming a steric block between P- and E-site tRNAs. G1575 is coordinated by a network of conserved Bud23 residues, overview |
