EC Number   |
General Stability |
Reference |
|---|
    2.3.1.57 | N1,N11-bis(ethyl)norspermine completely stabilizes the wild-type enzyme, which is degraded within 90 min in absence of N1,N11-bis(ethyl)norspermine, half-life: 22 min, half-life in presence of N1,N11-bis(ethyl)norspermine: 144 h, while for the mutant L156F half-life is 105 min and 154 min, respectively |
662129 |
| 2.3.1.57 | N1,N11-diethylnorspermine stabilizes |
487267 |
| 2.3.1.57 | native protein conformation is unstable, polyamine analogues such as N1,N12-bis(ethyl)spermine greatly stabilize, conformational changes caused by their binding prevent the efficient polyubiquination of enzyme and therefore its degradation |
487268 |
| 2.3.1.57 | PMSF and Brij 35 are essential to prevent rapid loss of activity of the enzyme preparation |
487259 |
| 2.3.1.57 | sensitive to incubation in dilute solutions, bovine serum albumin, 0.5 mg/ml, stabilizes |
487258 |
| 2.3.1.57 | unstable enzyme, glycerol 10% v/v or bovine serum albumin, 5 mg/ml, partially stabilizes |
487256 |
| 2.3.1.57 | unstable enzyme, spermidine stabilizes |
487257, 487258 |
