EC Number |
General Stability |
Reference |
---|
1.17.4.1 | dithiothreitol, 1 mM, stabilizes |
437911 |
1.17.4.1 | effector-binding subunit of mammalia is more sensitive to proteolysis by chymotrypsin, to heating at 55°C and to sulfhydryl reagents e.g. p-chloromercuribenzoate and N-ethylmaleimide, than the nonheme iron subunit, the latter is more sensitive to trypsin treatment |
437934 |
1.17.4.1 | glycerol and ATP required for stabilization |
437966 |
1.17.4.1 | partially purified enzyme is very unstable in solution, half-life at 0°C: less than 24 h |
437948 |
1.17.4.1 | protein concentrations above 5 mg/ml stabilizes |
437911 |
1.17.4.1 | quite susceptible to denaturation |
437974 |