1.1.1.4	(2R,3R)-2,3-butanediol + NAD+	-	Saccharomyces cerevisiae	(3R)-acetoin + NADH	-	r	165097	
1.1.1.4	(2R,3R)-2,3-butanediol + NADP+	no detectable activity with (2S,3S)-2,3-butanediol	Clostridium autoethanogenum	(R)-acetoin + NADPH + H+	-	r	436630	
1.1.1.4	(2R,3R)-butane-2,3-diol + NAD+	-	Paenibacillus polymyxa	(3R,3S)-acetoin + NADH + H+	-	r	421731	
1.1.1.4	(2R,3R)-butane-2,3-diol + NAD+	preferred substrate	Mycobacterium sp.	(3R,3S)-acetoin + NADH + H+	-	r	421731	
1.1.1.4	(2R,3R)-butane-2,3-diol + NAD+	Paenibacillus polymyxa ATCC 12321 has the ability to form almost exclusively the R isomer of 2,3-BDL (over 98%) when grown under anaerobic conditions	Paenibacillus polymyxa	(3R,3S)-acetoin + NADH + H+	-	r	421731	
1.1.1.4	(2R,3R)-butane-2,3-diol + NAD+	preferred substrate, very low activity with the (S)-enantiomer	Mycobacterium sp.	(3R,3S)-acetoin + NADH + H+	-	r	421731	
1.1.1.4	(2R,3R)-butane-2,3-diol + NAD+	sole product in the reduction reaction, preferred substrate in the oxidation reaction	Paenibacillus polymyxa	(3R,3S)-acetoin + NADH + H+	preferred substrate in the reduction reaction	r	421731	
1.1.1.4	(2R,3R)-butane-2,3-diol + NAD+	-	Paenibacillus polymyxa ZJ-9	(3R,3S)-acetoin + NADH + H+	-	r	421731	
1.1.1.4	(2R,3R)-butane-2,3-diol + NAD+	Paenibacillus polymyxa ATCC 12321 has the ability to form almost exclusively the R isomer of 2,3-BDL (over 98%) when grown under anaerobic conditions	Paenibacillus polymyxa ATCC 12321	(3R,3S)-acetoin + NADH + H+	-	r	421731	
1.1.1.4	(2R,3R)-butane-2,3-diol + NAD+	sole product in the reduction reaction, preferred substrate in the oxidation reaction	Paenibacillus polymyxa ATCC 12321	(3R,3S)-acetoin + NADH + H+	preferred substrate in the reduction reaction	r	421731	
1.1.1.4	(2R,3R)-butane-2,3-diol + NAD+	-	Klebsiella pneumoniae	(3R)-acetoin + NADH + H+	-	?	436208	
1.1.1.4	(2R,3R)-butane-2,3-diol + NAD+	-	Alkalihalobacillus clausii	(3R)-acetoin + NADH + H+	-	r	436208	
1.1.1.4	(2R,3R)-butane-2,3-diol + NAD+	-	Bacillus thuringiensis serovar kurstaki	(3R)-acetoin + NADH + H+	-	r	436208	
1.1.1.4	(2R,3R)-butane-2,3-diol + NAD+	-	Alkalihalobacillus clausii DSM 8716T	(3R)-acetoin + NADH + H+	-	r	436208	
1.1.1.4	(2R,3R)-butane-2,3-diol + NAD+	-	Klebsiella pneumoniae ATCC 25955	(3R)-acetoin + NADH + H+	-	?	436208	
1.1.1.4	(2R,3R)-butane-2,3-diol + NAD+	-	Bacillus thuringiensis serovar kurstaki ACCC 10066	(3R)-acetoin + NADH + H+	-	r	436208	
1.1.1.4	(2R,3R)-butane-2,3-diol + NAD+	-	Bacillus licheniformis	(R)-acetoin + NADH + H+	-	r	436209	
1.1.1.4	(2R,3R)-butane-2,3-diol + NAD+	-	Rhodococcus erythropolis	(R)-acetoin + NADH + H+	-	r	436209	
1.1.1.4	(2R,3R)-butane-2,3-diol + NAD+	-	Bacillus licheniformis MW3	(R)-acetoin + NADH + H+	-	r	436209	
1.1.1.4	(2R,3R)-butane-2,3-diol + NAD+	69.5% activity compared to (2R,3R)-butane-2,3-diol	Rhodococcus erythropolis	?	-	?	436631	
1.1.1.4	(2R,3S)-butane-2,3-diol + NAD+	-	Pseudomonas aeruginosa	(3R)-acetoin + NADH + H+	-	?	440031	
1.1.1.4	(2R,3S)-butane-2,3-diol + NAD+	-	Bacillus thuringiensis serovar kurstaki	(3R)-acetoin + NADH + H+	-	r	440031	
1.1.1.4	(2R,3S)-butane-2,3-diol + NAD+	slightly preferred substrate	Bacillus thuringiensis serovar kurstaki	(3R)-acetoin + NADH + H+	-	r	440031	
1.1.1.4	(2R,3S)-butane-2,3-diol + NAD+	stereoselective interconversion	Pseudomonas aeruginosa	(3R)-acetoin + NADH + H+	-	?	440031	
1.1.1.4	(2R,3S)-butane-2,3-diol + NAD+	-	Pseudomonas aeruginosa ATCC 15692	(3R)-acetoin + NADH + H+	-	?	440031	
1.1.1.4	(2R,3S)-butane-2,3-diol + NAD+	stereoselective interconversion	Pseudomonas aeruginosa ATCC 15692	(3R)-acetoin + NADH + H+	-	?	440031	
1.1.1.4	(2R,3S)-butane-2,3-diol + NAD+	-	Pseudomonas aeruginosa 1C	(3R)-acetoin + NADH + H+	-	?	440031	
1.1.1.4	(2R,3S)-butane-2,3-diol + NAD+	stereoselective interconversion	Pseudomonas aeruginosa 1C	(3R)-acetoin + NADH + H+	-	?	440031	
1.1.1.4	(2R,3S)-butane-2,3-diol + NAD+	-	Pseudomonas aeruginosa PRS 101	(3R)-acetoin + NADH + H+	-	?	440031	
1.1.1.4	(2R,3S)-butane-2,3-diol + NAD+	stereoselective interconversion	Pseudomonas aeruginosa PRS 101	(3R)-acetoin + NADH + H+	-	?	440031	
1.1.1.4	(2R,3S)-butane-2,3-diol + NAD+	-	Pseudomonas aeruginosa DSM 22644	(3R)-acetoin + NADH + H+	-	?	440031	
1.1.1.4	(2R,3S)-butane-2,3-diol + NAD+	stereoselective interconversion	Pseudomonas aeruginosa DSM 22644	(3R)-acetoin + NADH + H+	-	?	440031	
1.1.1.4	(2R,3S)-butane-2,3-diol + NAD+	-	Pseudomonas aeruginosa CIP 104116	(3R)-acetoin + NADH + H+	-	?	440031	
1.1.1.4	(2R,3S)-butane-2,3-diol + NAD+	stereoselective interconversion	Pseudomonas aeruginosa CIP 104116	(3R)-acetoin + NADH + H+	-	?	440031	
1.1.1.4	(2R,3S)-butane-2,3-diol + NAD+	-	Pseudomonas aeruginosa LMG 12228	(3R)-acetoin + NADH + H+	-	?	440031	
1.1.1.4	(2R,3S)-butane-2,3-diol + NAD+	stereoselective interconversion	Pseudomonas aeruginosa LMG 12228	(3R)-acetoin + NADH + H+	-	?	440031	
1.1.1.4	(2R,3S)-butane-2,3-diol + NAD+	-	Pseudomonas aeruginosa JCM 14847	(3R)-acetoin + NADH + H+	-	?	440031	
1.1.1.4	(2R,3S)-butane-2,3-diol + NAD+	stereoselective interconversion	Pseudomonas aeruginosa JCM 14847	(3R)-acetoin + NADH + H+	-	?	440031	
1.1.1.4	(2R,3S)-butane-2,3-diol + NAD+	-	Bacillus thuringiensis serovar kurstaki ACCC 10066	(3R)-acetoin + NADH + H+	-	r	440031	
1.1.1.4	(2R,3S)-butane-2,3-diol + NAD+	slightly preferred substrate	Bacillus thuringiensis serovar kurstaki ACCC 10066	(3R)-acetoin + NADH + H+	-	r	440031	
1.1.1.4	(2S)-acetoin + NADH + H+	the enzyme is not absolutely specific for (S)-acetoin, though this is the preferred substrate	Corynebacterium glutamicum	(2S,3S)-butane-2,3-diol + NAD+	-	?	436213	
1.1.1.4	(2S)-acetoin + NADH + H+	the enzyme is not absolutely specific for (S)-acetoin, though this is the preferred substrate	Corynebacterium glutamicum ATCC 13032	(2S,3S)-butane-2,3-diol + NAD+	-	?	436213	
1.1.1.4	(2S,3S)-butane-2,3-diol + NAD+	-	Corynebacterium glutamicum	(2S)-acetoin + NADH + H+	-	?	436215	
1.1.1.4	(2S,3S)-butane-2,3-diol + NAD+	very low activity	Bacillus licheniformis	(2S)-acetoin + NADH + H+	-	?	436215	
1.1.1.4	(2S,3S)-butane-2,3-diol + NAD+	very low activity	Bacillus licheniformis MW3	(2S)-acetoin + NADH + H+	-	?	436215	
1.1.1.4	(2S,3S)-butane-2,3-diol + NAD+	-	Corynebacterium glutamicum ATCC 13032	(2S)-acetoin + NADH + H+	-	?	436215	
1.1.1.4	(3R)-acetoin + NADH + H+	-	Saccharomyces cerevisiae	(2R,3R)-butane-2,3-diol + NAD+	-	?	403033	
1.1.1.4	(3R,3S)-acetoin + NADH	-	Saccharomyces cerevisiae	(2R,3R)-2,3-butanediol + meso-2,3-butanediol + NAD+	-	r	165098	
1.1.1.4	(3R,3S)-acetoin + NADH	the enzyme shows lower Km value and higher catalytic efficiency for (3S/3R)-acetoin in comparison to those for (2R,3R)-2,3-butanediol and meso-2,3-butanediol, the reduction reaction is preferred, low activity with (2R,3R)-2,3-butanediol	Serratia marcescens	(2R,3R)-2,3-butanediol + meso-2,3-butanediol + NAD+	-	r	165098	
1.1.1.4	(3R,3S)-acetoin + NADH	the enzyme shows lower Km value and higher catalytic efficiency for (3S/3R)-acetoin in comparison to those for (2R,3R)-2,3-butanediol and meso-2,3-butanediol, the reduction reaction is preferred, low activity with (2R,3R)-2,3-butanediol	Serratia marcescens H30	(2R,3R)-2,3-butanediol + meso-2,3-butanediol + NAD+	-	r	165098	
1.1.1.4	(3R,3S)-butane-2,3-diol + NAD+	-	Klebsiella pneumoniae	(3R,3S)-acetoin + NADH + H+	-	?	436637	
1.1.1.4	(3R,3S)-butane-2,3-diol + NAD+	-	Klebsiella pneumoniae ATCC 25955	(3R,3S)-acetoin + NADH + H+	-	?	436637	
1.1.1.4	(3S)-acetoin + NADH + H+	-	Saccharomyces cerevisiae	(2R,3S)-butane-2,3-diol + NAD+	-	?	403045	
1.1.1.4	(R)-1,2-propanediol + NAD+	73% activity compared to (2R,3R)-butane-2,3-diol in the oxidation reaction, very low activity with the (S)-enantiomer	Mycobacterium sp.	hydroxyacetone + NADH + H+	-	?	422212	
1.1.1.4	(R)-1-phenyl-1,2-ethanediol + NAD+	32.2% activity compared to (2R,3R)-butane-2,3-diol	Rhodococcus erythropolis	?	-	?	436652	
1.1.1.4	(R)-acetoin + NADH + H+	-	Bacillus licheniformis	(2R,3R)-butane-2,3-diol + NAD+	-	r	403105	
1.1.1.4	(R)-acetoin + NADH + H+	-	Saccharomyces cerevisiae	(2R,3R)-butane-2,3-diol + NAD+	-	?	403105	
1.1.1.4	(R)-acetoin + NADH + H+	 the activity of (R)-acetoin reduction is 7.7times higher than that of (2R,3R)-butane-2,3-diol oxidation at pH 7.0	Rhodococcus erythropolis	(2R,3R)-butane-2,3-diol + NAD+	-	r	403105	
1.1.1.4	(R)-acetoin + NADH + H+	-	Bacillus licheniformis MW3	(2R,3R)-butane-2,3-diol + NAD+	-	r	403105	
1.1.1.4	(R)-acetoin + NADH + H+	 the activity of (R)-acetoin reduction is 7.7times higher than that of (2R,3R)-butane-2,3-diol oxidation at pH 7.0	Rhodococcus erythropolis WZ010	(2R,3R)-butane-2,3-diol + NAD+	-	r	403105	
1.1.1.4	(R)-acetoin + NADH + H+	-	Bacillus subtilis	(R,R)-butane-2,3-diol + NAD+	-	r	436223	
1.1.1.4	(R)-acetoin + NADH + H+	-	Serratia marcescens	(R,R)-butane-2,3-diol + NAD+	-	r	436223	
1.1.1.4	(R)-acetoin + NADH + H+	-	Bacillus licheniformis	(R,R)-butane-2,3-diol + NAD+	-	r	436223	
1.1.1.4	(R)-acetoin + NADH + H+	-	Paenibacillus polymyxa	(R,R)-butane-2,3-diol + NAD+	-	r	436223	
1.1.1.4	(R)-acetoin + NADH + H+	-	Saccharomyces uvarum	(R,R)-butane-2,3-diol + NAD+	-	r	436223	
1.1.1.4	(R)-acetoin + NADH + H+	low activity	Corynebacterium glutamicum	(R,R)-butane-2,3-diol + NAD+	-	r	436223	
1.1.1.4	(R)-acetoin + NADH + H+	high level production of (R,R)-butane-2,3-diol	Paenibacillus polymyxa	(R,R)-butane-2,3-diol + NAD+	-	r	436223	
1.1.1.4	(R)-acetoin + NADH + H+	-	Bacillus subtilis 168	(R,R)-butane-2,3-diol + NAD+	-	r	436223	
1.1.1.4	(R)-acetoin + NADH + H+	low activity	Corynebacterium glutamicum ATCC 13032	(R,R)-butane-2,3-diol + NAD+	-	r	436223	
1.1.1.4	(R)-acetoin + NADH + H+	-	Paenibacillus polymyxa DSM 365	(R,R)-butane-2,3-diol + NAD+	-	r	436223	
1.1.1.4	(R)-acetoin + NADH + H+	high level production of (R,R)-butane-2,3-diol	Paenibacillus polymyxa DSM 365	(R,R)-butane-2,3-diol + NAD+	-	r	436223	
1.1.1.4	(R)-acetoin + NADH + H+	-	Saccharomyces uvarum WY1	(R,R)-butane-2,3-diol + NAD+	-	r	436223	
1.1.1.4	(R)-acetoin + NADH + H+	-	Serratia marcescens MG1	(R,R)-butane-2,3-diol + NAD+	-	r	436223	
1.1.1.4	(R)-acetoin + NADPH + H+	wild type enzyme does not use NADPH as coenzyme	Saccharomyces cerevisiae	(2R,3R)-butane-2,3-diol + NADP+	-	?	403106	
1.1.1.4	(R)-acetoin + NADPH + H+	-	Clostridium autoethanogenum	(2R,3R)-2,3-butanediol + NAD+	-	?	436658	
1.1.1.4	(R,R)-butane-2,3-diol + NAD+	-	Bacillus subtilis	(R)-acetoin + NADH + H+	-	?	394598	
1.1.1.4	(R,R)-butane-2,3-diol + NAD+	-	Bacillus subtilis	(R)-acetoin + NADH + H+	-	r	394598	
1.1.1.4	(R,R)-butane-2,3-diol + NAD+	-	Serratia marcescens	(R)-acetoin + NADH + H+	-	r	394598	
1.1.1.4	(R,R)-butane-2,3-diol + NAD+	-	Enterococcus faecium	(R)-acetoin + NADH + H+	-	r	394598	
1.1.1.4	(R,R)-butane-2,3-diol + NAD+	-	Corynebacterium glutamicum	(R)-acetoin + NADH + H+	-	r	394598	
1.1.1.4	(R,R)-butane-2,3-diol + NAD+	-	Weizmannia coagulans	(R)-acetoin + NADH + H+	-	?	394598	
1.1.1.4	(R,R)-butane-2,3-diol + NAD+	-	Bacillus licheniformis	(R)-acetoin + NADH + H+	-	r	394598	
1.1.1.4	(R,R)-butane-2,3-diol + NAD+	-	Enterococcus durans	(R)-acetoin + NADH + H+	-	r	394598	
1.1.1.4	(R,R)-butane-2,3-diol + NAD+	-	Klebsiella oxytoca	(R)-acetoin + NADH + H+	-	r	394598	
1.1.1.4	(R,R)-butane-2,3-diol + NAD+	-	Bacillus subtilis subsp. subtilis	(R)-acetoin + NADH + H+	-	r	394598	
1.1.1.4	(R,R)-butane-2,3-diol + NAD+	-	Paenibacillus brasilensis	(R)-acetoin + NADH + H+	-	?	394598	
1.1.1.4	(R,R)-butane-2,3-diol + NAD+	-	Saccharomyces cerevisiae	(R)-acetoin + NADH + H+	-	?	394598	
1.1.1.4	(R,R)-butane-2,3-diol + NAD+	-	Bacillus amyloliquefaciens	(R)-acetoin + NADH + H+	-	r	394598	
1.1.1.4	(R,R)-butane-2,3-diol + NAD+	-	Pseudomonas aeruginosa	(R)-acetoin + NADH + H+	-	r	394598	
1.1.1.4	(R,R)-butane-2,3-diol + NAD+	preferred substrate, 100% activity	Bacillus sp. (in: Bacteria)	(R)-acetoin + NADH + H+	-	?	394598	
1.1.1.4	(R,R)-butane-2,3-diol + NAD+	effects of growth substrate on enzyme activity in vivo, overview	Enterococcus faecium	(R)-acetoin + NADH + H+	-	r	394598	
1.1.1.4	(R,R)-butane-2,3-diol + NAD+	effects of growth substrate on enzyme activity in vivo, overview	Enterococcus durans	(R)-acetoin + NADH + H+	-	r	394598	
1.1.1.4	(R,R)-butane-2,3-diol + NAD+	-	Pseudomonas aeruginosa ATCC 15692	(R)-acetoin + NADH + H+	-	r	394598	
1.1.1.4	(R,R)-butane-2,3-diol + NAD+	-	Bacillus subtilis 168	(R)-acetoin + NADH + H+	-	r	394598	
1.1.1.4	(R,R)-butane-2,3-diol + NAD+	preferred substrate, 100% activity	Bacillus sp. (in: Bacteria) BRC1	(R)-acetoin + NADH + H+	-	?	394598	
1.1.1.4	(R,R)-butane-2,3-diol + NAD+	-	Pseudomonas aeruginosa 1C	(R)-acetoin + NADH + H+	-	r	394598	
1.1.1.4	(R,R)-butane-2,3-diol + NAD+	-	Corynebacterium glutamicum ATCC 13032	(R)-acetoin + NADH + H+	-	r	394598	
1.1.1.4	(R,R)-butane-2,3-diol + NAD+	-	Enterococcus durans Ov 421	(R)-acetoin + NADH + H+	-	r	394598	
1.1.1.4	(R,R)-butane-2,3-diol + NAD+	effects of growth substrate on enzyme activity in vivo, overview	Enterococcus durans Ov 421	(R)-acetoin + NADH + H+	-	r	394598	
1.1.1.4	(R,R)-butane-2,3-diol + NAD+	-	Pseudomonas aeruginosa PRS 101	(R)-acetoin + NADH + H+	-	r	394598	
1.1.1.4	(R,R)-butane-2,3-diol + NAD+	-	Saccharomyces cerevisiae YBA_08	(R)-acetoin + NADH + H+	-	?	394598	
1.1.1.4	(R,R)-butane-2,3-diol + NAD+	-	Pseudomonas aeruginosa DSM 22644	(R)-acetoin + NADH + H+	-	r	394598	
1.1.1.4	(R,R)-butane-2,3-diol + NAD+	-	Pseudomonas aeruginosa CIP 104116	(R)-acetoin + NADH + H+	-	r	394598	
1.1.1.4	(R,R)-butane-2,3-diol + NAD+	-	Pseudomonas aeruginosa LMG 12228	(R)-acetoin + NADH + H+	-	r	394598	
1.1.1.4	(R,R)-butane-2,3-diol + NAD+	-	Enterococcus faecium ET C9	(R)-acetoin + NADH + H+	-	r	394598	
1.1.1.4	(R,R)-butane-2,3-diol + NAD+	effects of growth substrate on enzyme activity in vivo, overview	Enterococcus faecium ET C9	(R)-acetoin + NADH + H+	-	r	394598	
1.1.1.4	(R,R)-butane-2,3-diol + NAD+	-	Paenibacillus brasilensis PB24	(R)-acetoin + NADH + H+	-	?	394598	
1.1.1.4	(R,R)-butane-2,3-diol + NAD+	-	Serratia marcescens MG1	(R)-acetoin + NADH + H+	-	r	394598	
1.1.1.4	(R,R)-butane-2,3-diol + NAD+	-	Bacillus amyloliquefaciens UCMB5033	(R)-acetoin + NADH + H+	-	r	394598	
1.1.1.4	(R,R)-butane-2,3-diol + NAD+	-	Bacillus subtilis BS168D	(R)-acetoin + NADH + H+	-	r	394598	
1.1.1.4	(R,R)-butane-2,3-diol + NAD+	-	Pseudomonas aeruginosa JCM 14847	(R)-acetoin + NADH + H+	-	r	394598	
1.1.1.4	(R,R)-butane-2,3-diol + NAD+	-	Bacillus subtilis WN1038	(R)-acetoin + NADH + H+	-	?	394598	
1.1.1.4	(R,R)-butane-2,3-diol + NAD+	-	Klebsiella oxytoca ME-303	(R)-acetoin + NADH + H+	-	r	394598	
1.1.1.4	(R,R)-butane-2,3-diol + NAD+	-	Bacillus subtilis subsp. subtilis 168	(R)-acetoin + NADH + H+	-	r	394598	
1.1.1.4	(R,R)-butane-2,3-diol + NAD+	-	Weizmannia coagulans 2-6	(R)-acetoin + NADH + H+	-	?	394598	
1.1.1.4	(R,R)-butane-2,3-diol + NAD+	stereoselective interconversion	Pseudomonas aeruginosa	(3R)-acetoin + NADH + H+	-	r	458789	
1.1.1.4	(R,R)-butane-2,3-diol + NAD+	stereoselective interconversion	Pseudomonas aeruginosa ATCC 15692	(3R)-acetoin + NADH + H+	-	r	458789	
1.1.1.4	(R,R)-butane-2,3-diol + NAD+	stereoselective interconversion	Pseudomonas aeruginosa 1C	(3R)-acetoin + NADH + H+	-	r	458789	
1.1.1.4	(R,R)-butane-2,3-diol + NAD+	stereoselective interconversion	Pseudomonas aeruginosa PRS 101	(3R)-acetoin + NADH + H+	-	r	458789	
1.1.1.4	(R,R)-butane-2,3-diol + NAD+	stereoselective interconversion	Pseudomonas aeruginosa DSM 22644	(3R)-acetoin + NADH + H+	-	r	458789	
1.1.1.4	(R,R)-butane-2,3-diol + NAD+	stereoselective interconversion	Pseudomonas aeruginosa CIP 104116	(3R)-acetoin + NADH + H+	-	r	458789	
1.1.1.4	(R,R)-butane-2,3-diol + NAD+	stereoselective interconversion	Pseudomonas aeruginosa LMG 12228	(3R)-acetoin + NADH + H+	-	r	458789	
1.1.1.4	(R,R)-butane-2,3-diol + NAD+	stereoselective interconversion	Pseudomonas aeruginosa JCM 14847	(3R)-acetoin + NADH + H+	-	r	458789	
1.1.1.4	(R,S)-3-hydroxy-2-pentanone + NADH	-	Saccharomyces cerevisiae	2,3-pentanediol + NAD+	-	r	165103	
1.1.1.4	(R,S)-4-hydroxy-3-pentanone + NADH	-	Saccharomyces cerevisiae	2,3-pentanediol + NAD+	-	r	165102	
1.1.1.4	1,2-butanediol + NAD+	-	Saccharomyces cerevisiae	?	-	?	165101	
1.1.1.4	1,2-hexanediol + NAD+	50% activity compared to (2R,3R)-butane-2,3-diol in the oxidation reaction	Mycobacterium sp.	? + NADH + H+	-	?	422242	
1.1.1.4	1,2-pentandiol + NAD+	-	Paenibacillus polymyxa	? + NADH + H+	-	r	422243	
1.1.1.4	1,2-pentanediol + NAD+	74% activity compared to (2R,3R)-butane-2,3-diol in the oxidation reaction	Mycobacterium sp.	? + NADH + H+	-	?	422244	
1.1.1.4	1,2-propandiol + NAD+	-	Paenibacillus polymyxa	? + NADH + H+	-	r	422246	
1.1.1.4	1,2-propanediol + NAD+	-	Saccharomyces cerevisiae	?	-	?	165100	
1.1.1.4	1,2-propanediol + NAD+	-	Paenibacillus polymyxa	?	-	r	165100	
1.1.1.4	1,3-dihydroxyacetone + NADH + H+	low activity	Bacillus licheniformis	?	-	?	436685	
1.1.1.4	1,4-butanediol + NAD+	low activity	Serratia marcescens	?	-	r	353240	
1.1.1.4	1,4-butanediol + NAD+	low activity	Serratia marcescens H30	?	-	r	353240	
1.1.1.4	1-hydroxy-2-butanone + NADH	-	Saccharomyces cerevisiae	?	-	?	165104	
1.1.1.4	1-hydroxy-2-butanone + NADH + H+	low activity	Bacillus licheniformis	?	-	?	436695	
1.1.1.4	1-hydroxy-2-butanone + NADH + H+	-	Pseudomonas aeruginosa	? + NAD+	-	?	458836	
1.1.1.4	1-hydroxy-2-propanone + NADH + H+	-	Saccharomyces cerevisiae	propane-1,2-diol + NAD+	-	r	165099	
1.1.1.4	2,2,2-trifluoroacetophenone + NADH + H+	28% activity compared to (R)-acetoin	Rhodococcus erythropolis	?	-	?	403357	
1.1.1.4	2,2,2-trifluoroacetophenone + NADH + H+	28% activity compared to (R)-acetoin	Rhodococcus erythropolis WZ010	?	-	?	403357	
1.1.1.4	2,3-butanediol + NAD+	-	Klebsiella aerogenes	acetoin + NADH	-	r	165092	
1.1.1.4	2,3-butanediol + NAD+	-	Lactococcus lactis	acetoin + NADH	-	r	165092	
1.1.1.4	2,3-butanediol + NAD+	D(-)-2,3-butandiol	Bacillus subtilis	acetoin + NADH	-	r	165092	
1.1.1.4	2,3-butanediol + NAD+	D(-)-2,3-butandiol	Pseudomonas sp.	acetoin + NADH	-	?	165092	
1.1.1.4	2,3-butanediol + NAD+	D(-)-2,3-butandiol	Bacillus sp. (in: Bacteria)	acetoin + NADH	-	?	165092	
1.1.1.4	2,3-butanediol + NAD+	D(-)-2,3-butandiol	Paenibacillus polymyxa	acetoin + NADH	-	r	165092	
1.1.1.4	2,3-butanediol + NAD+	D(-)-2,3-butandiol	Aeromonas hydrophila	acetoin + NADH	-	r	165092	
1.1.1.4	2,3-butanediol + NAD+	D(-)-2,3-butandiol	Micrococcus sp.	acetoin + NADH	-	?	165092	
1.1.1.4	2,3-butanediol + NAD+	D(-)-2,3-butandiol	Gluconobacter oxydans	acetoin + NADH	-	r	165092	
1.1.1.4	2,3-butanediol + NAD+	D(-)-2,3-butandiol	Enterobacter sp.	acetoin + NADH	-	?	165092	
1.1.1.4	2,3-butanediol + NAD+	D(-)-2,3-butandiol	Brevibacillus sp.	acetoin + NADH	-	?	165092	
1.1.1.4	2,3-butanediol + NAD+	2,3-butanediol without specification of stereochemistry	Paracoccus denitrificans	acetoin + NADH	-	?	165092	
1.1.1.4	2,3-butanediol + NAD+	2,3-butanediol without specification of stereochemistry	Saccharomyces cerevisiae	acetoin + NADH	-	r	165092	
1.1.1.4	2,3-butanediol + NAD+	oxidation occurs selectively at the (R)-center of 2,3-butanediol	Saccharomyces cerevisiae	acetoin + NADH	-	r	165092	
1.1.1.4	2,3-butanediol + NAD+	-	Pyrococcus furiosus	acetoin + NADH + H+	-	r	383627	
1.1.1.4	2,3-hexanedione + NADH + H+	small amounts of (2R)-2-hydroxy-3-hexanone are produced as by-product	Alkalihalobacillus clausii	(3R)-3-hydroxy-2-hexanone + NAD+	-	r	458749	
1.1.1.4	2,3-hexanedione + NADH + H+	small amounts of (2R)-2-hydroxy-3-hexanone are produced as by-product	Alkalihalobacillus clausii DSM 8716T	(3R)-3-hydroxy-2-hexanone + NAD+	-	r	458749	
1.1.1.4	2,3-pentandione + NADH + H+	-	Pseudomonas aeruginosa	? + NAD+	-	?	458915	
1.1.1.4	2,3-pentanediol + NAD+	-	Saccharomyces cerevisiae	4-hydroxy-3-pentanone + 3-hydroxy-2-pentanone + NADH	-	?	165107	
1.1.1.4	2,3-pentanediol + NAD+	racemate	Saccharomyces cerevisiae	4-hydroxy-3-pentanone + 3-hydroxy-2-pentanone + NADH	-	r	165107	
1.1.1.4	2,3-pentanediol + NAD+	-	Klebsiella aerogenes	?	-	r	451056	
1.1.1.4	2,3-pentanedione + NADH + H+	 small amounts of 2,3-pentanediol are produced as by-product	Alkalihalobacillus clausii	3-hydroxy-2-pentanone + NAD+	-	r	407823	
1.1.1.4	2,3-pentanedione + NADH + H+	 small amounts of 2,3-pentanediol are produced as by-product	Alkalihalobacillus clausii DSM 8716T	3-hydroxy-2-pentanone + NAD+	-	r	407823	
1.1.1.4	2-butanone + NADP+	-	Clostridium autoethanogenum	2-butanol + NADPH + H+	-	?	403500	
1.1.1.4	2-hydroxyacetophenone + NADH + H+	8.7% activity compared to (R)-acetoin	Rhodococcus erythropolis	?	-	?	436775	
1.1.1.4	2-hydroxyacetophenone + NADH + H+	-	Bacillus subtilis subsp. subtilis	(R)-1-phenyl-1,2-ethanediol + NAD+	-	?	458424	
1.1.1.4	2-hydroxyacetophenone + NADH + H+	over 99% enantiomeric excess	Bacillus subtilis subsp. subtilis	(R)-1-phenyl-1,2-ethanediol + NAD+	-	?	458424	
1.1.1.4	2-hydroxyacetophenone + NADH + H+	-	Bacillus subtilis subsp. subtilis 168	(R)-1-phenyl-1,2-ethanediol + NAD+	-	?	458424	
1.1.1.4	2-hydroxyacetophenone + NADH + H+	over 99% enantiomeric excess	Bacillus subtilis subsp. subtilis 168	(R)-1-phenyl-1,2-ethanediol + NAD+	-	?	458424	
1.1.1.4	2-octanone + NADH + H+	9.3% activity compared to (R)-acetoin	Rhodococcus erythropolis	?	-	?	436789	
1.1.1.4	2-pentanone + NADH + H+	-	Pyrococcus furiosus	(S)-2-pentanol + NAD+	-	?	383780	
1.1.1.4	2-propanol + NAD+	-	Paenibacillus polymyxa	2-propanone + NADH + H+	-	r	383787	
1.1.1.4	2-propanol + NAD+	-	Paenibacillus polymyxa ATCC 12321	2-propanone + NADH + H+	-	r	383787	
1.1.1.4	3,4-hexanedione + 2 NADH + 2 H+	-	Alkalihalobacillus clausii	(3R,4R)-3,4-hexanediol + 2 NAD+	-	r	461401	
1.1.1.4	3-methyl-2-butenal + NADH + H+	16.7% activity compared to (R)-acetoin	Rhodococcus erythropolis	?	-	?	436878	
1.1.1.4	5-methyl-2,3-hexanedione + NADH + H+	small amounts of 5-methyl-2-hydroxy-3-hexanone and traces of (3S)-5-methyl-3-hydroxy-2-hexanone are produced as by-products	Alkalihalobacillus clausii	(3R)-5-methyl-3-hydroxy-2-hexanone + NAD+	-	r	458748	
1.1.1.4	5-methyl-2,3-hexanedione + NADH + H+	small amounts of 5-methyl-2-hydroxy-3-hexanone and traces of (3S)-5-methyl-3-hydroxy-2-hexanone are produced as by-products	Alkalihalobacillus clausii DSM 8716T	(3R)-5-methyl-3-hydroxy-2-hexanone + NAD+	-	r	458748	
1.1.1.4	acetaldehyde + NADH + H+	-	Saccharomyces cerevisiae	ethanol + NAD+	-	?	139564	
1.1.1.4	acetaldehyde + NADH + H+	-	Saccharomyces cerevisiae YBA_08	ethanol + NAD+	-	?	139564	
1.1.1.4	acetaldehyde + NADPH + H+	-	Clostridium autoethanogenum	ethanol + NADP+	-	?	139551	
1.1.1.4	acetaldehyde + NADPH + H+	-	Saccharomyces cerevisiae	ethanol + NADP+	-	?	139551	
1.1.1.4	acetaldehyde + NADPH + H+	-	Saccharomyces cerevisiae YBA_08	ethanol + NADP+	-	?	139551	
1.1.1.4	acetoin + 2 NADPH + 2 H+	-	Clostridium autoethanogenum	(2R,3R)-2,3-butanediol + meso-2,3-butanediol + 2 NADP+	-	r	436971	
1.1.1.4	acetoin + NAD+	18% of the activity with 2,3-butanediol, in the reverse reaction 150% of the activity with acetoin	Pyrococcus furiosus	diacetyl + NADH	-	r	370844	
1.1.1.4	acetoin + NADH	-	Bacillus subtilis	2,3-butanediol + NAD+	-	r	404183	
1.1.1.4	acetoin + NADH + H+	-	Bacillus subtilis	2,3-butanediol + NAD+	-	r	165093	
1.1.1.4	acetoin + NADH + H+	-	Saccharomyces cerevisiae	2,3-butanediol + NAD+	-	r	165093	
1.1.1.4	acetoin + NADH + H+	-	Klebsiella aerogenes	2,3-butanediol + NAD+	-	r	165093	
1.1.1.4	acetoin + NADH + H+	-	Paenibacillus polymyxa	2,3-butanediol + NAD+	-	r	165093	
1.1.1.4	acetoin + NADH + H+	-	Aeromonas hydrophila	2,3-butanediol + NAD+	-	r	165093	
1.1.1.4	acetoin + NADH + H+	-	Gluconobacter oxydans	2,3-butanediol + NAD+	-	r	165093	
1.1.1.4	acetoin + NADH + H+	-	Lactococcus lactis	2,3-butanediol + NAD+	enzyme 1, only meso-2,3-butanediol is formed, enzyme 2 gives mixture of meso- and optical isomers	r	165093	
1.1.1.4	acetoin + NADH + H+	-	Escherichia coli	(R,R)-butanediol + NAD+	-	?	439010	
1.1.1.4	acetoin + NADH + H+	-	Escherichia coli BW25113	(R,R)-butanediol + NAD+	-	?	439010	
1.1.1.4	acetoin + NADPH + H+	-	Escherichia coli	(R,R)-butanediol + NADP+	-	?	439011	
1.1.1.4	acetoin + NADPH + H+	-	Escherichia coli BW25113	(R,R)-butanediol + NADP+	-	?	439011	
1.1.1.4	acetone + NADPH + H+	-	Clostridium autoethanogenum	propan-2-ol + NADP+	-	?	396197	
1.1.1.4	butane-1,2-diol + NAD+	low activity	Bacillus licheniformis	?	-	?	437245	
1.1.1.4	butane-1,2-diol + NAD+	76.6% activity compared to (R,R)-butane-2,3-diol	Bacillus sp. (in: Bacteria)	?	-	?	437245	
1.1.1.4	butane-1,2-diol + NAD+	76.6% activity compared to (R,R)-butane-2,3-diol	Bacillus sp. (in: Bacteria) BRC1	?	-	?	437245	
1.1.1.4	butane-1,2-diol + NAD+	-	Pseudomonas aeruginosa	? + NADH + H+	-	?	459500	
1.1.1.4	butane-1,3-diol + NAD+	14.7% activity compared to (2R,3R)-butane-2,3-diol	Rhodococcus erythropolis	?	-	?	437246	
1.1.1.4	butane-1,4-diol + NAD+	2.9% activity compared to (R,R)-butane-2,3-diol	Bacillus sp. (in: Bacteria)	?	-	?	437247	
1.1.1.4	butanone + NADH + H+	low activity	Bacillus licheniformis	?	-	?	437249	
1.1.1.4	butanone + NADPH + H+	-	Clostridium autoethanogenum	butan-2-ol + NADP+	-	?	426052	
1.1.1.4	cyclohexanone + NADH	38% of the activity with acetoin	Pyrococcus furiosus	?	-	?	384492	
1.1.1.4	diacetyl + 2 NADH + 2 H+	-	Pseudomonas aeruginosa	(2R,3R)-butane-2,3-diol + 2 NAD+	-	r	459616	
1.1.1.4	diacetyl + NADH	-	Saccharomyces cerevisiae	2,3-butanediol + NAD+	-	?	165106	
1.1.1.4	diacetyl + NADH	-	Klebsiella aerogenes	2,3-butanediol + NAD+	-	?	165106	
1.1.1.4	diacetyl + NADH	-	Lactococcus lactis	2,3-butanediol + NAD+	enzyme 1, only meso-2,3-butanediol is formed, enzyme 2 gives mixture of meso- and optical isomers	r	165106	
1.1.1.4	diacetyl + NADH + H+	-	Paenibacillus polymyxa	acetoin + NAD+	-	r	394828	
1.1.1.4	diacetyl + NADH + H+	low activity	Bacillus licheniformis	acetoin + NAD+	-	?	394828	
1.1.1.4	diacetyl + NADH + H+	91% activity compared to (3R,3S)-acetoin in the reduction reaction, cf. EC 1.1.1.303	Paenibacillus polymyxa	acetoin + NAD+	-	?	394828	
1.1.1.4	diacetyl + NADH + H+	88.2% activity compared to acetoin	Bacillus sp. (in: Bacteria)	acetoin + NAD+	-	?	394828	
1.1.1.4	diacetyl + NADH + H+	diacetyl can be converted into 2,3-butanediol via acetoin by the enzyme	Serratia marcescens	acetoin + NAD+	-	?	394828	
1.1.1.4	diacetyl + NADH + H+	88.2% activity compared to acetoin	Bacillus sp. (in: Bacteria) BRC1	acetoin + NAD+	-	?	394828	
1.1.1.4	diacetyl + NADH + H+	low activity	Bacillus licheniformis MW3	acetoin + NAD+	-	?	394828	
1.1.1.4	diacetyl + NADH + H+	diacetyl can be converted into 2,3-butanediol via acetoin by the enzyme	Serratia marcescens H30	acetoin + NAD+	-	?	394828	
1.1.1.4	diacetyl + NADH + H+	91% activity compared to (3R,3S)-acetoin in the reduction reaction, cf. EC 1.1.1.303	Paenibacillus polymyxa ATCC 12321	acetoin + NAD+	-	?	394828	
1.1.1.4	diacetyl + NADH + H+	69.3% activity compared to (R)-acetoin	Rhodococcus erythropolis	(R)-acetoin + NAD+	-	r	409283	
1.1.1.4	diacetyl + NADH + H+	69.3% activity compared to (R)-acetoin	Rhodococcus erythropolis WZ010	(R)-acetoin + NAD+	-	r	409283	
1.1.1.4	diacetyl + NADH + H+	-	Bacillus subtilis	(3S)-acetoin + NAD+	-	ir	423306	
1.1.1.4	diacetyl + NADH + H+	-	Bacillus subtilis	(3S)-acetoin + NAD+	-	r	423306	
1.1.1.4	diacetyl + NADH + H+	-	Serratia marcescens	(3S)-acetoin + NAD+	-	r	423306	
1.1.1.4	diacetyl + NADH + H+	-	Bacillus subtilis 168	(3S)-acetoin + NAD+	-	r	423306	
1.1.1.4	diacetyl + NADH + H+	-	Bacillus subtilis 168	(3S)-acetoin + NAD+	-	ir	423306	
1.1.1.4	diacetyl + NADH + H+	-	Serratia marcescens MG1	(3S)-acetoin + NAD+	-	r	423306	
1.1.1.4	diacetyl + NADH + H+	-	Corynebacterium glutamicum	(2S)-acetoin + NAD+	-	?	437389	
1.1.1.4	diacetyl + NADH + H+	-	Corynebacterium glutamicum ATCC 13032	(2S)-acetoin + NAD+	-	?	437389	
1.1.1.4	diacetyl + NADH + H+	-	Pseudomonas aeruginosa	(3R)-acetoin + NAD+	-	r	459617	
1.1.1.4	dihydroxyacetone + NADH	36% of the activity with acetoin	Pyrococcus furiosus	?	-	?	384613	
1.1.1.4	dihydroxyacetone phosphate + NADH	82% of the activity with acetoin	Pyrococcus furiosus	?	-	?	384614	
1.1.1.4	ethyl 4-chloro-3-hydroxybutyrate + NAD+	12.2% activity compared to (2R,3R)-butane-2,3-diol	Rhodococcus erythropolis	?	-	?	437542	
1.1.1.4	ethyl pyruvate + NADH	-	Klebsiella aerogenes	?	-	?	165109	
1.1.1.4	formaldehyde + NADH + H+	-	Saccharomyces cerevisiae	? + NAD+	-	?	459683	
1.1.1.4	formaldehyde + NADPH + H+	-	Saccharomyces cerevisiae	? + NADP+	-	?	459684	
1.1.1.4	furfural + NADH + H+	-	Saccharomyces cerevisiae	(furan-2-yl)methanol + NAD+	-	?	423533	
1.1.1.4	furfural + NADH + H+	-	Saccharomyces cerevisiae YBA_08	(furan-2-yl)methanol + NAD+	-	?	423533	
1.1.1.4	furfural + NADPH + H+	-	Saccharomyces cerevisiae	(furan-2-yl)methanol + NADP+	-	?	459687	
1.1.1.4	glycerol + NAD+	-	Klebsiella pneumoniae	?	-	?	165124	
1.1.1.4	glycerol + NAD+	16.3% activity compared to (2R,3R)-butane-2,3-diol	Rhodococcus erythropolis	?	-	?	165124	
1.1.1.4	glycerol + NAD+	16.3% activity compared to (2R,3R)-butane-2,3-diol	Rhodococcus erythropolis WZ010	?	-	?	165124	
1.1.1.4	glycerol + NAD+	-	Klebsiella pneumoniae ATCC 25955	?	-	?	165124	
1.1.1.4	glycerol + NAD+	-	Paenibacillus polymyxa	? + NADH + H+	-	r	423632	
1.1.1.4	glycolaldehyde + NADH + H+	-	Saccharomyces cerevisiae	? + NAD+	-	?	439586	
1.1.1.4	hexaldehyde + NADH + H+	-	Saccharomyces cerevisiae	? + NAD+	-	?	459738	
1.1.1.4	hexane-2,5-dione + NADH + H+	low activity	Bacillus licheniformis	?	-	?	437609	
1.1.1.4	hydroxyacetone + NADH	-	Saccharomyces cerevisiae	1,2-propanediol	-	?	165105	
1.1.1.4	isobutanol + NAD+	0.4% activity compared to (R,R)-butane-2,3-diol	Bacillus sp. (in: Bacteria)	?	-	?	437622	
1.1.1.4	isopropanol + NAD+	low activity	Serratia marcescens	?	-	r	439630	
1.1.1.4	isopropanol + NAD+	low activity	Serratia marcescens H30	?	-	r	439630	
1.1.1.4	meso-2,3-butanediol + NAD+	-	Pseudomonas sp.	D-(-)-acetoin + NADH	-	?	165094	
1.1.1.4	meso-2,3-butanediol + NAD+	-	Bacillus sp. (in: Bacteria)	D-(-)-acetoin + NADH	-	?	165094	
1.1.1.4	meso-2,3-butanediol + NAD+	-	Micrococcus sp.	D-(-)-acetoin + NADH	-	?	165094	
1.1.1.4	meso-2,3-butanediol + NAD+	-	Enterobacter sp.	D-(-)-acetoin + NADH	-	?	165094	
1.1.1.4	meso-2,3-butanediol + NAD+	-	Brevibacillus sp.	D-(-)-acetoin + NADH	-	?	165094	
1.1.1.4	meso-2,3-butanediol + NAD+	-	Saccharomyces cerevisiae	D-(-)-acetoin + NADH	(3S)-acetoin	r	165094	
1.1.1.4	meso-2,3-butanediol + NAD+	-	Paenibacillus polymyxa	?	-	r	438394	
1.1.1.4	meso-butane-2,3-diol + NAD+	-	Paenibacillus polymyxa	acetoin + NADH + H+	-	r	423831	
1.1.1.4	meso-butane-2,3-diol + NAD+	61% activity compared to (2R,3R)-butane-2,3-diol in the oxidation reaction	Mycobacterium sp.	acetoin + NADH + H+	-	r	423831	
1.1.1.4	meso-butane-2,3-diol + NAD+	72% activity compared to (2R,3R)-butane-2,3-diol in the oxidation reaction	Paenibacillus polymyxa	acetoin + NADH + H+	-	r	423831	
1.1.1.4	meso-butane-2,3-diol + NAD+	93.6% activity compared to (R,R)-butane-2,3-diol	Bacillus sp. (in: Bacteria)	acetoin + NADH + H+	-	?	423831	
1.1.1.4	meso-butane-2,3-diol + NAD+	93.6% activity compared to (R,R)-butane-2,3-diol	Bacillus sp. (in: Bacteria) BRC1	acetoin + NADH + H+	-	?	423831	
1.1.1.4	meso-butane-2,3-diol + NAD+	-	Paenibacillus polymyxa ZJ-9	acetoin + NADH + H+	-	r	423831	
1.1.1.4	meso-butane-2,3-diol + NAD+	-	Klebsiella pneumoniae	(3S)-acetoin + NADH + H+	-	?	437687	
1.1.1.4	meso-butane-2,3-diol + NAD+	-	Klebsiella pneumoniae ATCC 25955	(3S)-acetoin + NADH + H+	-	?	437687	
1.1.1.4	meso-butane-2,3-diol + NAD+	-	Haloarcula marismortui	(R)-acetoin + NADH + H+	-	?	437688	
1.1.1.4	methyl glyoxal + NADH	-	Klebsiella aerogenes	?	-	?	165108	
1.1.1.4	methyl pyruvate + NADH	-	Klebsiella aerogenes	?	-	?	165110	
1.1.1.4	additional information	3S)-2,3-butanediol, (3R/3S)-acetoin, glycerol, sorbitol or xylitol are no substrates	Saccharomyces cerevisiae	?	-	?	89	
1.1.1.4	additional information	substrate specificity, overview. No or poor activity with ethanol, ethylene glycol, diethylene glycol, 1-propanol, 2-propanol, 1,3-propanediol, glycerol, dipropylene glycol, 1-butanol, 1,4-butanediol, 1,2,4-butanetriol, (2S,3S)-butane-2,3-diol, 1,5-pentanediol, 2,4-pentanediol, 3-methyl-1,5-pentandiol, and 1,2,6-hexantriol	Mycobacterium sp.	?	-	?	89	
1.1.1.4	additional information	the enzyme is also active in reduction reaction with diacetyl (91% compared to (2R,3S)-acetoin) and glyceraldehyde-3-phosphate (12% compared to (2R,3S)-acetoin), cf. EC 1.1.1.303 and EC 1.1.1.8, respectively, but not with dihydroxyacetone phosphate. It shows low activity in oxidation reaction with ethanol, n-propanol, n-butanol, 1,3-propanediol, and 1,5-pentanediol	Paenibacillus polymyxa	?	-	?	89	
1.1.1.4	additional information	enzyme is a strictly NADPH-dependent primary-secondary alcohol dehydrogenase able to reduce acetoin to 2,3-butanediol. The enzyme accepts a range of 2-, 3-, and 4-carbon substrates, including the nonphysiological ketones acetone and butanone	Clostridium autoethanogenum	?	-	?	89	
1.1.1.4	additional information	no activity with (2S,3S)-butane-2,3-diol or diacetyl	Klebsiella pneumoniae	?	-	?	89	
1.1.1.4	additional information	no activity with (S,S)-butane-2,3-diol	Bacillus sp. (in: Bacteria)	?	-	?	89	
1.1.1.4	additional information	no activity with acetoin plus NAD+ or (2S,3S)-butane-2,3-diol witrh NAD+, or (S)-1-phenyl-1,2-ethanediol plus NAD+. 5% or less activity with 2-butanol, ethyl lactate, isopropanol, 1-butanol, cyclohexanol, 2-pentanol, 2-octanol, acetophenone, and 4-hydroxy-2-butanone	Rhodococcus erythropolis	?	-	?	89	
1.1.1.4	additional information	no activity with meso-butane-2,3-diol, glycerol, glycerol-3-phosphate, 1,3-dihydroxyacetone, dihydroxyacetonephosphate, glyceraldehyde-3-phosphate, 2-butanol, and 1,3-butanediol	Bacillus licheniformis	?	-	?	89	
1.1.1.4	additional information	the purified enzyme glycerol dehydrogenase, GDH EC 1.1.1.6, also catalyzes the interconversion of (3S)-acetoin/meso-2,3-butanediol and (3R)-acetoin/(2R,3R)-2,3-butanediol. (2S,3S)-2,3-Butanediol is not a substrate for the GDH at all. Also no activity with 1-propanol, 1-butanol, isopentanol, ethylene glycol, ethanol, and (3S/3R)-acetoin in the oxidation reaction, and poor activity with formaldehyde in the reduction reaction	Serratia marcescens	?	-	?	89	
1.1.1.4	additional information	an enantiocomplementary carbonyl reductase, 2,3-butanediol dehydrogenase (BDHA) from Bacillus subtilis is discovered to convert 2-hydroxyacetophenone (2-HAP) to (R)-1-phenyl-1,2-ethanediol ((R)-PED) with excellent stereochemical selectivity. No activity with NADPH	Bacillus subtilis subsp. subtilis	?	-	-	89	
1.1.1.4	additional information	enzyme Bdh1p exhibits (2R,3R)-2,3-butanediol dehydrogenase activity, but Bdh1p also exhibit reductive activities towards lignocellulosic aldehyde inhibitors, such as acetaldehyde, glycolaldehyde, and furfural. No activity for glycolaldehyde or hexaldehyde with NADPH. Propionaldehyde is a poor substrate, no activity with glutaraldehyde	Saccharomyces cerevisiae	?	-	-	89	
1.1.1.4	additional information	enzyme Bdh1p exhibits (2R,3R)-2,3-butanediol dehydrogenase activity, but Bdh2p also exhibit reductive activities towards lignocellulosic aldehyde inhibitors, such as acetaldehyde, glycolaldehyde, and furfural. No activity for furfural, propionaldehyde, or hexaldehyde with NADPH. Glutaraldehyde is a poor substrate	Saccharomyces cerevisiae	?	-	-	89	
1.1.1.4	additional information	enzyme BtBDH is active with meso-2,3-butanediol and (2R,3R)-2,3-butanediol, whereas no activity is observed with (2S,3S)-2,3-butanediol. BtBDH shows similar oxidative activity toward meso-2,3-butanediol and (2R,3R)-2,3-butanediol, and it exhibits a 3fold higher reduction activity toward acetoin compared to diacetyl	Bacillus thuringiensis serovar kurstaki	?	-	-	89	
1.1.1.4	additional information	no activity with (2S,3S)-2,3-butane-2,3-diol, but the (2R,3R)-2,3-butanediol dehydrogenase is also active with meso-2,3-butanediol. No activity with 2-butanol, 1,3-butanediol, 1,2-pentanediol, 1,3-propanediol, and glycerol in the oxidation reaction. And no activity with 2,4-pentanedione, butanone, 2,5-hexanedione, n-butanal and 1,3-dihydroxypropanone in the reduction reaction. Substrate specificity, overview. (2R,3R)-2,3-BDH reduces diacetyl into (3R)-acetoin and (2R,3R)-2,3-BD, while racemic acetoin is reduced into (2R,3R)-2,3-BD and meso-2,3-BD	Pseudomonas aeruginosa	?	-	-	89	
1.1.1.4	additional information	production of R,R- and meso-2,3-BDO by Paenibacillus brasilensis strain PB24 grown in the modified YEPD medium, pH 6.3, 32°C, up to 72 h	Paenibacillus brasilensis	?	-	-	89	
1.1.1.4	additional information	substrate specificity analysis, overview. Enzyme BcBDH catalyzes the selective asymmetric reduction of prochiral 1,2-diketones to the corresponding HK and, in some cases, the reduction of the same to the corresponding 1,2-diols. Aliphatic diketones, like 2,3-pentanedione, 2,3-hexanedione, 5-methyl-2,3-hexanedione, 3,4-hexanedione, and 2,3-heptanedione are well transformed. In addition, surprisingly alkyl phenyl dicarbonyls, like 2-hydroxy-1-phenylpropan-1-one and phenylglyoxal are accepted, whereas their derivatives with two phenyl groups are not substrates. The biocatalytic reduction of 5-methyl-2,3-hexanedione to mainly 5-methyl-3-hydroxy-2-hexanone with only small amounts of 5-methyl-2-hydroxy-3-hexanone within an enzyme membrane reactor is demonstrated. Stereoselectivity and conversion is analyzed by carrying out the reduction reaction of selected diketones and alpha-hydroxyketones	Alkalihalobacillus clausii	?	-	-	89	
1.1.1.4	additional information	no activity with (2S,3S)-2,3-butane-2,3-diol, but the (2R,3R)-2,3-butanediol dehydrogenase is also active with meso-2,3-butanediol. No activity with 2-butanol, 1,3-butanediol, 1,2-pentanediol, 1,3-propanediol, and glycerol in the oxidation reaction. And no activity with 2,4-pentanedione, butanone, 2,5-hexanedione, n-butanal and 1,3-dihydroxypropanone in the reduction reaction. Substrate specificity, overview. (2R,3R)-2,3-BDH reduces diacetyl into (3R)-acetoin and (2R,3R)-2,3-BD, while racemic acetoin is reduced into (2R,3R)-2,3-BD and meso-2,3-BD	Pseudomonas aeruginosa ATCC 15692	?	-	-	89	
1.1.1.4	additional information	no activity with (S,S)-butane-2,3-diol	Bacillus sp. (in: Bacteria) BRC1	?	-	?	89	
1.1.1.4	additional information	no activity with (2S,3S)-2,3-butane-2,3-diol, but the (2R,3R)-2,3-butanediol dehydrogenase is also active with meso-2,3-butanediol. No activity with 2-butanol, 1,3-butanediol, 1,2-pentanediol, 1,3-propanediol, and glycerol in the oxidation reaction. And no activity with 2,4-pentanedione, butanone, 2,5-hexanedione, n-butanal and 1,3-dihydroxypropanone in the reduction reaction. Substrate specificity, overview. (2R,3R)-2,3-BDH reduces diacetyl into (3R)-acetoin and (2R,3R)-2,3-BD, while racemic acetoin is reduced into (2R,3R)-2,3-BD and meso-2,3-BD	Pseudomonas aeruginosa 1C	?	-	-	89	
1.1.1.4	additional information	no activity with meso-butane-2,3-diol, glycerol, glycerol-3-phosphate, 1,3-dihydroxyacetone, dihydroxyacetonephosphate, glyceraldehyde-3-phosphate, 2-butanol, and 1,3-butanediol	Bacillus licheniformis MW3	?	-	?	89	
1.1.1.4	additional information	no activity with (2S,3S)-2,3-butane-2,3-diol, but the (2R,3R)-2,3-butanediol dehydrogenase is also active with meso-2,3-butanediol. No activity with 2-butanol, 1,3-butanediol, 1,2-pentanediol, 1,3-propanediol, and glycerol in the oxidation reaction. And no activity with 2,4-pentanedione, butanone, 2,5-hexanedione, n-butanal and 1,3-dihydroxypropanone in the reduction reaction. Substrate specificity, overview. (2R,3R)-2,3-BDH reduces diacetyl into (3R)-acetoin and (2R,3R)-2,3-BD, while racemic acetoin is reduced into (2R,3R)-2,3-BD and meso-2,3-BD	Pseudomonas aeruginosa PRS 101	?	-	-	89	
1.1.1.4	additional information	the purified enzyme glycerol dehydrogenase, GDH EC 1.1.1.6, also catalyzes the interconversion of (3S)-acetoin/meso-2,3-butanediol and (3R)-acetoin/(2R,3R)-2,3-butanediol. (2S,3S)-2,3-Butanediol is not a substrate for the GDH at all. Also no activity with 1-propanol, 1-butanol, isopentanol, ethylene glycol, ethanol, and (3S/3R)-acetoin in the oxidation reaction, and poor activity with formaldehyde in the reduction reaction	Serratia marcescens H30	?	-	?	89	
1.1.1.4	additional information	enzyme Bdh1p exhibits (2R,3R)-2,3-butanediol dehydrogenase activity, but Bdh1p also exhibit reductive activities towards lignocellulosic aldehyde inhibitors, such as acetaldehyde, glycolaldehyde, and furfural. No activity for glycolaldehyde or hexaldehyde with NADPH. Propionaldehyde is a poor substrate, no activity with glutaraldehyde	Saccharomyces cerevisiae YBA_08	?	-	-	89	
1.1.1.4	additional information	enzyme Bdh1p exhibits (2R,3R)-2,3-butanediol dehydrogenase activity, but Bdh2p also exhibit reductive activities towards lignocellulosic aldehyde inhibitors, such as acetaldehyde, glycolaldehyde, and furfural. No activity for furfural, propionaldehyde, or hexaldehyde with NADPH. Glutaraldehyde is a poor substrate	Saccharomyces cerevisiae YBA_08	?	-	-	89	
1.1.1.4	additional information	substrate specificity analysis, overview. Enzyme BcBDH catalyzes the selective asymmetric reduction of prochiral 1,2-diketones to the corresponding HK and, in some cases, the reduction of the same to the corresponding 1,2-diols. Aliphatic diketones, like 2,3-pentanedione, 2,3-hexanedione, 5-methyl-2,3-hexanedione, 3,4-hexanedione, and 2,3-heptanedione are well transformed. In addition, surprisingly alkyl phenyl dicarbonyls, like 2-hydroxy-1-phenylpropan-1-one and phenylglyoxal are accepted, whereas their derivatives with two phenyl groups are not substrates. The biocatalytic reduction of 5-methyl-2,3-hexanedione to mainly 5-methyl-3-hydroxy-2-hexanone with only small amounts of 5-methyl-2-hydroxy-3-hexanone within an enzyme membrane reactor is demonstrated. Stereoselectivity and conversion is analyzed by carrying out the reduction reaction of selected diketones and alpha-hydroxyketones	Alkalihalobacillus clausii DSM 8716T	?	-	-	89	
1.1.1.4	additional information	no activity with (2S,3S)-2,3-butane-2,3-diol, but the (2R,3R)-2,3-butanediol dehydrogenase is also active with meso-2,3-butanediol. No activity with 2-butanol, 1,3-butanediol, 1,2-pentanediol, 1,3-propanediol, and glycerol in the oxidation reaction. And no activity with 2,4-pentanedione, butanone, 2,5-hexanedione, n-butanal and 1,3-dihydroxypropanone in the reduction reaction. Substrate specificity, overview. (2R,3R)-2,3-BDH reduces diacetyl into (3R)-acetoin and (2R,3R)-2,3-BD, while racemic acetoin is reduced into (2R,3R)-2,3-BD and meso-2,3-BD	Pseudomonas aeruginosa DSM 22644	?	-	-	89	
1.1.1.4	additional information	no activity with (2S,3S)-2,3-butane-2,3-diol, but the (2R,3R)-2,3-butanediol dehydrogenase is also active with meso-2,3-butanediol. No activity with 2-butanol, 1,3-butanediol, 1,2-pentanediol, 1,3-propanediol, and glycerol in the oxidation reaction. And no activity with 2,4-pentanedione, butanone, 2,5-hexanedione, n-butanal and 1,3-dihydroxypropanone in the reduction reaction. Substrate specificity, overview. (2R,3R)-2,3-BDH reduces diacetyl into (3R)-acetoin and (2R,3R)-2,3-BD, while racemic acetoin is reduced into (2R,3R)-2,3-BD and meso-2,3-BD	Pseudomonas aeruginosa CIP 104116	?	-	-	89	
1.1.1.4	additional information	no activity with (2S,3S)-2,3-butane-2,3-diol, but the (2R,3R)-2,3-butanediol dehydrogenase is also active with meso-2,3-butanediol. No activity with 2-butanol, 1,3-butanediol, 1,2-pentanediol, 1,3-propanediol, and glycerol in the oxidation reaction. And no activity with 2,4-pentanedione, butanone, 2,5-hexanedione, n-butanal and 1,3-dihydroxypropanone in the reduction reaction. Substrate specificity, overview. (2R,3R)-2,3-BDH reduces diacetyl into (3R)-acetoin and (2R,3R)-2,3-BD, while racemic acetoin is reduced into (2R,3R)-2,3-BD and meso-2,3-BD	Pseudomonas aeruginosa LMG 12228	?	-	-	89	
1.1.1.4	additional information	production of R,R- and meso-2,3-BDO by Paenibacillus brasilensis strain PB24 grown in the modified YEPD medium, pH 6.3, 32°C, up to 72 h	Paenibacillus brasilensis PB24	?	-	-	89	
1.1.1.4	additional information	no activity with acetoin plus NAD+ or (2S,3S)-butane-2,3-diol witrh NAD+, or (S)-1-phenyl-1,2-ethanediol plus NAD+. 5% or less activity with 2-butanol, ethyl lactate, isopropanol, 1-butanol, cyclohexanol, 2-pentanol, 2-octanol, acetophenone, and 4-hydroxy-2-butanone	Rhodococcus erythropolis WZ010	?	-	?	89	
1.1.1.4	additional information	no activity with (2S,3S)-butane-2,3-diol or diacetyl	Klebsiella pneumoniae ATCC 25955	?	-	?	89	
1.1.1.4	additional information	no activity with (2S,3S)-2,3-butane-2,3-diol, but the (2R,3R)-2,3-butanediol dehydrogenase is also active with meso-2,3-butanediol. No activity with 2-butanol, 1,3-butanediol, 1,2-pentanediol, 1,3-propanediol, and glycerol in the oxidation reaction. And no activity with 2,4-pentanedione, butanone, 2,5-hexanedione, n-butanal and 1,3-dihydroxypropanone in the reduction reaction. Substrate specificity, overview. (2R,3R)-2,3-BDH reduces diacetyl into (3R)-acetoin and (2R,3R)-2,3-BD, while racemic acetoin is reduced into (2R,3R)-2,3-BD and meso-2,3-BD	Pseudomonas aeruginosa JCM 14847	?	-	-	89	
1.1.1.4	additional information	an enantiocomplementary carbonyl reductase, 2,3-butanediol dehydrogenase (BDHA) from Bacillus subtilis is discovered to convert 2-hydroxyacetophenone (2-HAP) to (R)-1-phenyl-1,2-ethanediol ((R)-PED) with excellent stereochemical selectivity. No activity with NADPH	Bacillus subtilis subsp. subtilis 168	?	-	-	89	
1.1.1.4	additional information	enzyme BtBDH is active with meso-2,3-butanediol and (2R,3R)-2,3-butanediol, whereas no activity is observed with (2S,3S)-2,3-butanediol. BtBDH shows similar oxidative activity toward meso-2,3-butanediol and (2R,3R)-2,3-butanediol, and it exhibits a 3fold higher reduction activity toward acetoin compared to diacetyl	Bacillus thuringiensis serovar kurstaki ACCC 10066	?	-	-	89	
1.1.1.4	additional information	the enzyme is also active in reduction reaction with diacetyl (91% compared to (2R,3S)-acetoin) and glyceraldehyde-3-phosphate (12% compared to (2R,3S)-acetoin), cf. EC 1.1.1.303 and EC 1.1.1.8, respectively, but not with dihydroxyacetone phosphate. It shows low activity in oxidation reaction with ethanol, n-propanol, n-butanol, 1,3-propanediol, and 1,5-pentanediol	Paenibacillus polymyxa ATCC 12321	?	-	?	89	
1.1.1.4	n-butylaldehyde + NADH + H+	low activity	Bacillus licheniformis	?	-	?	437720	
1.1.1.4	pentane-1,2-diol + NAD+	very low activity	Bacillus licheniformis	?	-	?	437815	
1.1.1.4	pentane-2,3-dione + NADH + H+	low activity	Bacillus licheniformis	?	-	?	437816	
1.1.1.4	pentane-2,4-dione + NADH + H+	low activity	Bacillus licheniformis	?	-	?	437817	
1.1.1.4	propane-1,2-diol + NAD+	low activity	Bacillus licheniformis	?	-	?	437843	
1.1.1.4	propane-1,2-diol + NAD+	58.6% activity compared to (R,R)-butane-2,3-diol	Bacillus sp. (in: Bacteria)	?	-	?	437843	
1.1.1.4	propane-1,2-diol + NAD+	-	Pseudomonas aeruginosa	? + NADH + H+	-	?	459875	
1.1.1.4	propane-1,3-diol + NAD+	1.4% activity compared to (R,R)-butane-2,3-diol	Bacillus sp. (in: Bacteria)	?	-	?	437844	
1.1.1.4	propionaldehyde + NADH + H+	-	Saccharomyces cerevisiae	? + NAD+	-	?	459877	
1.1.1.4	pyruvic aldehyde + NADH	81% of the activity with acetoin	Pyrococcus furiosus	?	-	?	385654	
1.1.1.4	rac acetoin + NADH + H+	-	Pseudomonas aeruginosa	(2R,3R)-butane-2,3-diol + NAD+	-	?	459884	
1.1.1.4	rac acetoin + NADH + H+	-	Pseudomonas aeruginosa	(2R,3S)-butane-2,3-diol + NAD+	-	?	459885	
1.1.1.4	rac-1,2-propanediol + NAD+	-	Mycobacterium sp.	hydroxyacetone + NADH + H+	-	?	422031	
1.1.1.4	rac-1,2-propanediol + NAD+	68% activity compared to (2R,3R)-butane-2,3-diol in the oxidation reaction	Mycobacterium sp.	hydroxyacetone + NADH + H+	-	?	422031	
1.1.1.4	sodium lactate + NAD+	10.2% activity compared to (2R,3R)-butane-2,3-diol	Rhodococcus erythropolis	?	-	?	437904