2.7.1.28 0.00574 - ATP mutant enzyme S446A, with dihydroxyacetone as cosubstrate, at pH 7.5 and 37°C 730035 2.7.1.28 0.006 - dihydroxyacetone pH 7.5, 23-24°C, at 2 mM ATPMg2- 641263 2.7.1.28 0.0071 - D-glyceraldehyde mutant enzyme S446A, at pH 7.5 and 37°C 730035 2.7.1.28 0.008 - D-glyceraldehyde pH 7.5, 23-24°C, cosubstrate 1.0 mM MgATP2- 641263 2.7.1.28 0.0099 - dihydroxyacetone mutant enzyme S446A, at pH 7.5 and 37°C 730035 2.7.1.28 0.01 - glyceraldehyde pH 7.0, 22°C 641262 2.7.1.28 0.012 - D-glyceraldehyde pH 7.5, 23-24°C, cosubstrate 0.5 mM MgATP2- 641263 2.7.1.28 0.0137 - D-glyceraldehyde mutant enzyme T112A, at pH 7.5 and 37°C 730035 2.7.1.28 0.015 - D-glyceraldehyde mutant enzyme K204A, at pH 7.5 and 37°C 730035 2.7.1.28 0.0155 - dihydroxyacetone wild type enzyme, at pH 7.5 and 37°C 730035 2.7.1.28 0.0162 - D-glyceraldehyde mutant enzyme C404A, at pH 7.5 and 37°C 730035 2.7.1.28 0.0181 - D-glyceraldehyde wild type enzyme, at pH 7.5 and 37°C 730035 2.7.1.28 0.019 - D-glyceraldehyde pH 7.5, 23-24°C, cosubstrate 2.0 mM MgATP2- 641263 2.7.1.28 0.0191 - dihydroxyacetone mutant enzyme C404A, at pH 7.5 and 37°C 730035 2.7.1.28 0.0252 - dihydroxyacetone mutant enzyme K204A, at pH 7.5 and 37°C 730035 2.7.1.28 0.0352 - ATP mutant enzyme S446A, with D-glyceraldehyde as cosubstrate, at pH 7.5 and 37°C 730035 2.7.1.28 0.0432 - ATP wild type enzyme, with dihydroxyacetone as cosubstrate, at pH 7.5 and 37°C 730035 2.7.1.28 0.0624 - ATP wild type enzyme, with D-glyceraldehyde as cosubstrate, at pH 7.5 and 37°C 730035 2.7.1.28 0.0637 - ATP mutant enzyme K204A, with dihydroxyacetone as cosubstrate, at pH 7.5 and 37°C 730035 2.7.1.28 0.0663 - dihydroxyacetone mutant enzyme T112A, at pH 7.5 and 37°C 730035 2.7.1.28 0.0696 - ATP mutant enzyme K204A, with D-glyceraldehyde as cosubstrate, at pH 7.5 and 37°C 730035 2.7.1.28 0.0806 - ATP mutant enzyme T112A, with dihydroxyacetone as cosubstrate, at pH 7.5 and 37°C 730035 2.7.1.28 0.0853 - ATP mutant enzyme T112A, with D-glyceraldehyde as cosubstrate, at pH 7.5 and 37°C 730035 2.7.1.28 0.0996 - ATP mutant enzyme C404A, with dihydroxyacetone as cosubstrate, at pH 7.5 and 37°C 730035 2.7.1.28 0.16 - ATP ATP in form of MgATP2- 641263 2.7.1.28 0.16 - ATP pH 7.5, 23-24°C, 11.2 mM free Mg2+ 641263 2.7.1.28 0.183 - ATP mutant enzyme C404A, with D-glyceraldehyde as cosubstrate,at pH 7.5 and 37°C 730035 2.7.1.28 0.2 - ATP ATP in form of MgATP2- 641263 2.7.1.28 0.2 - ATP pH 7.5, 23-24°C, 1.11 mM free Mg2+ 641263 2.7.1.28 0.24 - ATP ATP in form of MgATP2- 641263 2.7.1.28 0.24 - ATP pH 7.5, 23-24°C, 0.34 mM free Mg2+ 641263