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Literature summary extracted from

  • Chauliac, D.; Wang, Q.; St John, F.J.; Jones, G.; Hurlbert, J.C.; Ingram, L.O.; Shanmugam, K.T.
    Kinetic characterization and structure analysis of an altered polyol dehydrogenase with D-lactate dehydrogenase activity (2020), Protein Sci., 29, 2387-2397 .
    View publication on PubMedView publication on EuropePMC

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.1.1.6 structure of mutant D121N/F245S Weizmannia coagulans

Protein Variants

EC Number Protein Variants Comment Organism
1.1.1.6 D121N mutation lowers the activity of the enzyme with most all the tested substrates relative to the native enzyme Weizmannia coagulans
1.1.1.6 D121N/F245S mutant acquires D-lactate dehydrogenase activiy, the alteration increases the capacity of the glycerol binding site and facilitated hydrogen bonding between the S245 gamma-O and the C1 carboxylate of pyruvate Weizmannia coagulans
1.1.1.6 F245S mutant acquires D-lactate dehydrogenase activiy, the alteration increases the capacity of the glycerol binding site and facilitated hydrogen bonding between the S245 gamma-O and the C1 carboxylate of pyruvate Weizmannia coagulans

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.6 0.05
-
NADH wild-type, pH 5.4, 55°C Weizmannia coagulans
1.1.1.6 0.1
-
NADH mutant D121N, pH 5.4, 55°C Weizmannia coagulans
1.1.1.6 0.1
-
NADH mutant F245S, pH 5.4, 55°C Weizmannia coagulans
1.1.1.6 0.3
-
NADH mutant /D121NF245S, pH 5.9, 55°C Weizmannia coagulans
1.1.1.6 0.4
-
NAD+ wild-type, pH 8.9, 55°C Weizmannia coagulans
1.1.1.6 0.7
-
NAD+ mutant F245S, pH 8.9, 55°C Weizmannia coagulans
1.1.1.6 1
-
D-1,2-propanediol wild-type, pH 8.9, 55°C Weizmannia coagulans
1.1.1.6 1.5
-
NAD+ mutant D121N, pH 8.9, 55°C Weizmannia coagulans
1.1.1.6 2.3
-
NAD+ mutant /D121NF245S, pH 8.9, 55°C Weizmannia coagulans
1.1.1.6 3
-
1,2-Butanediol wild-type, pH 8.9, 55°C Weizmannia coagulans
1.1.1.6 4
-
1,2-Butanediol mutant D121N, pH 8.9, 55°C Weizmannia coagulans
1.1.1.6 4
-
glycerol wild-type, pH 8.9, 55°C Weizmannia coagulans
1.1.1.6 4.5
-
D-1,2-propanediol mutant F245S, pH 8.9, 55°C Weizmannia coagulans
1.1.1.6 6.5
-
D-2,3-butanediol mutant F245S, pH 8.9, 55°C Weizmannia coagulans
1.1.1.6 8
-
D-1,2-propanediol mutant /D121NF245S, pH 8.9, 55°C Weizmannia coagulans
1.1.1.6 17
-
D-2,3-butanediol wild-type, pH 8.9, 55°C Weizmannia coagulans
1.1.1.6 19
-
D-1,2-propanediol mutant D121N, pH 8.9, 55°C Weizmannia coagulans
1.1.1.6 23.5
-
1,2-Butanediol mutant F245S, pH 8.9, 55°C Weizmannia coagulans
1.1.1.6 39
-
D-2,3-butanediol mutant /D121NF245S, pH 8.9, 55°C Weizmannia coagulans
1.1.1.6 41
-
glycerol mutant F245S, pH 8.9, 55°C Weizmannia coagulans
1.1.1.6 44
-
1,2-Butanediol mutant /D121NF245S, pH 8.9, 55°C Weizmannia coagulans
1.1.1.6 54.5
-
pyruvate mutant F245S, pH 5.4, 55°C Weizmannia coagulans
1.1.1.6 55.5
-
pyruvate mutant /D121NF245S, pH 5.9, 55°C Weizmannia coagulans
1.1.1.6 60.5
-
pyruvate mutant D121N, pH 5.4, 55°C Weizmannia coagulans
1.1.1.6 90
-
D-2,3-butanediol mutant D121N, pH 8.9, 55°C Weizmannia coagulans
1.1.1.6 148
-
glycerol mutant /D121NF245S, pH 8.9, 55°C Weizmannia coagulans
1.1.1.6 195
-
glycerol mutant D121N, pH 8.9, 55°C Weizmannia coagulans
1.1.1.6 207
-
pyruvate wild-type, pH 5.4, 55°C Weizmannia coagulans

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.1.1.6 Zn2+ 1.9fold increase in activity in presence of Zn2+ Weizmannia coagulans

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.6 86000
-
gel filtration Weizmannia coagulans

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.6 Weizmannia coagulans A0A150JSL8
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.6 1,2-butanediol + NAD+
-
Weizmannia coagulans 1-hydroxy-2-butanone + NADH + H+
-
?
1.1.1.6 1,2-ethanediol + NAD+
-
Weizmannia coagulans hydroxyacetaldehyde + NADH + H+
-
?
1.1.1.6 1,3-butanediol + NAD+
-
Weizmannia coagulans 4-hydroxy-2-butanone + NADH + H+
-
?
1.1.1.6 1,4-butanediol + NAD+
-
Weizmannia coagulans ? + NADH + H+
-
?
1.1.1.6 D-1,2-propanediol + NAD+
-
Weizmannia coagulans hydroxyacetone + NADH + H+
-
?
1.1.1.6 D-2,3-butanediol + NAD+
-
Weizmannia coagulans ? + NADH + H+
-
?
1.1.1.6 glycerol + NAD+
-
Weizmannia coagulans glycerone + NADH + H+
-
?
1.1.1.6 L-1,2-propanediol + NAD+
-
Weizmannia coagulans hydroxyacetone + NADH + H+
-
?
1.1.1.6 L-2,3-butanediol + NAD+
-
Weizmannia coagulans ? + NADH + H+
-
?
1.1.1.6 pyruvate + NADH + H+
-
Weizmannia coagulans D-lactate + NADH
-
?

Subunits

EC Number Subunits Comment Organism
1.1.1.6 dimer 2 * 39000, calculated from sequence Weizmannia coagulans

Synonyms

EC Number Synonyms Comment Organism
1.1.1.6 B4100_2156
-
Weizmannia coagulans

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.1.6 40 65 mutant F245S, pH 5, reduction of pyruvate Weizmannia coagulans
1.1.1.6 60
-
mutant D121N/F245S Weizmannia coagulans
1.1.1.6 65 75 wild-type Weizmannia coagulans

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.1.1.6 70
-
1 h, 95% loss of activity Weizmannia coagulans

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.1.1.6 1.5
-
pyruvate mutant D121N, pH 5.4, 55°C Weizmannia coagulans
1.1.1.6 10.5
-
D-2,3-butanediol mutant D121N, pH 8.9, 55°C Weizmannia coagulans
1.1.1.6 10.5
-
pyruvate wild-type, pH 5.4, 55°C Weizmannia coagulans
1.1.1.6 11
-
glycerol mutant /D121NF245S, pH 8.9, 55°C Weizmannia coagulans
1.1.1.6 29
-
D-1,2-propanediol mutant D121N, pH 8.9, 55°C Weizmannia coagulans
1.1.1.6 42.5
-
D-1,2-propanediol mutant /D121NF245S, pH 8.9, 55°C Weizmannia coagulans
1.1.1.6 42.5
-
glycerol mutant F245S, pH 8.9, 55°C Weizmannia coagulans
1.1.1.6 50
-
1,2-Butanediol mutant /D121NF245S, pH 8.9, 55°C Weizmannia coagulans
1.1.1.6 50
-
glycerol mutant D121N, pH 8.9, 55°C Weizmannia coagulans
1.1.1.6 50.5
-
D-2,3-butanediol wild-type, pH 8.9, 55°C Weizmannia coagulans
1.1.1.6 57.5
-
D-2,3-butanediol mutant /D121NF245S, pH 8.9, 55°C Weizmannia coagulans
1.1.1.6 66.5
-
1,2-Butanediol mutant D121N, pH 8.9, 55°C Weizmannia coagulans
1.1.1.6 71
-
1,2-Butanediol mutant F245S, pH 8.9, 55°C Weizmannia coagulans
1.1.1.6 87.5
-
D-1,2-propanediol mutant F245S, pH 8.9, 55°C Weizmannia coagulans
1.1.1.6 92
-
D-1,2-propanediol wild-type, pH 8.9, 55°C Weizmannia coagulans
1.1.1.6 117.5
-
glycerol wild-type, pH 8.9, 55°C Weizmannia coagulans
1.1.1.6 120.5
-
1,2-Butanediol wild-type, pH 8.9, 55°C Weizmannia coagulans
1.1.1.6 136
-
D-2,3-butanediol mutant F245S, pH 8.9, 55°C Weizmannia coagulans
1.1.1.6 165
-
pyruvate mutant /D121NF245S, pH 5.9, 55°C Weizmannia coagulans
1.1.1.6 352
-
pyruvate mutant F245S, pH 5.4, 55°C Weizmannia coagulans

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.6 5
-
mutant F245S, reduction of pyruvate Weizmannia coagulans
1.1.1.6 9
-
wild-type Weizmannia coagulans

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.1.1.6 0.02
-
pyruvate mutant D121N, pH 5.4, 55°C Weizmannia coagulans
1.1.1.6 0.05
-
pyruvate wild-type, pH 5.4, 55°C Weizmannia coagulans
1.1.1.6 0.1
-
glycerol mutant /D121NF245S, pH 8.9, 55°C Weizmannia coagulans
1.1.1.6 0.1
-
D-2,3-butanediol mutant D121N, pH 8.9, 55°C Weizmannia coagulans
1.1.1.6 0.3
-
glycerol mutant D121N, pH 8.9, 55°C Weizmannia coagulans
1.1.1.6 1
-
1,2-Butanediol mutant /D121NF245S, pH 8.9, 55°C Weizmannia coagulans
1.1.1.6 1
-
glycerol mutant F245S, pH 8.9, 55°C Weizmannia coagulans
1.1.1.6 1.5
-
D-2,3-butanediol mutant /D121NF245S, pH 8.9, 55°C Weizmannia coagulans
1.1.1.6 1.5
-
D-1,2-propanediol mutant D121N, pH 8.9, 55°C Weizmannia coagulans
1.1.1.6 3
-
pyruvate mutant /D121NF245S, pH 5.9, 55°C Weizmannia coagulans
1.1.1.6 3
-
1,2-Butanediol mutant F245S, pH 8.9, 55°C Weizmannia coagulans
1.1.1.6 3
-
D-2,3-butanediol wild-type, pH 8.9, 55°C Weizmannia coagulans
1.1.1.6 5.5
-
D-1,2-propanediol mutant /D121NF245S, pH 8.9, 55°C Weizmannia coagulans
1.1.1.6 6.5
-
pyruvate mutant F245S, pH 5.4, 55°C Weizmannia coagulans
1.1.1.6 15.5
-
1,2-Butanediol mutant D121N, pH 8.9, 55°C Weizmannia coagulans
1.1.1.6 20
-
D-1,2-propanediol mutant F245S, pH 8.9, 55°C Weizmannia coagulans
1.1.1.6 21
-
D-2,3-butanediol mutant F245S, pH 8.9, 55°C Weizmannia coagulans
1.1.1.6 30
-
glycerol wild-type, pH 8.9, 55°C Weizmannia coagulans
1.1.1.6 40
-
1,2-Butanediol wild-type, pH 8.9, 55°C Weizmannia coagulans
1.1.1.6 92
-
D-1,2-propanediol wild-type, pH 8.9, 55°C Weizmannia coagulans