EC Number | Crystallization (Comment) | Organism |
---|---|---|
1.1.1.6 | structure of mutant D121N/F245S | Weizmannia coagulans |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
1.1.1.6 | D121N | mutation lowers the activity of the enzyme with most all the tested substrates relative to the native enzyme | Weizmannia coagulans |
1.1.1.6 | D121N/F245S | mutant acquires D-lactate dehydrogenase activiy, the alteration increases the capacity of the glycerol binding site and facilitated hydrogen bonding between the S245 gamma-O and the C1 carboxylate of pyruvate | Weizmannia coagulans |
1.1.1.6 | F245S | mutant acquires D-lactate dehydrogenase activiy, the alteration increases the capacity of the glycerol binding site and facilitated hydrogen bonding between the S245 gamma-O and the C1 carboxylate of pyruvate | Weizmannia coagulans |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.1.1.6 | 0.05 | - |
NADH | wild-type, pH 5.4, 55°C | Weizmannia coagulans | |
1.1.1.6 | 0.1 | - |
NADH | mutant D121N, pH 5.4, 55°C | Weizmannia coagulans | |
1.1.1.6 | 0.1 | - |
NADH | mutant F245S, pH 5.4, 55°C | Weizmannia coagulans | |
1.1.1.6 | 0.3 | - |
NADH | mutant /D121NF245S, pH 5.9, 55°C | Weizmannia coagulans | |
1.1.1.6 | 0.4 | - |
NAD+ | wild-type, pH 8.9, 55°C | Weizmannia coagulans | |
1.1.1.6 | 0.7 | - |
NAD+ | mutant F245S, pH 8.9, 55°C | Weizmannia coagulans | |
1.1.1.6 | 1 | - |
D-1,2-propanediol | wild-type, pH 8.9, 55°C | Weizmannia coagulans | |
1.1.1.6 | 1.5 | - |
NAD+ | mutant D121N, pH 8.9, 55°C | Weizmannia coagulans | |
1.1.1.6 | 2.3 | - |
NAD+ | mutant /D121NF245S, pH 8.9, 55°C | Weizmannia coagulans | |
1.1.1.6 | 3 | - |
1,2-Butanediol | wild-type, pH 8.9, 55°C | Weizmannia coagulans | |
1.1.1.6 | 4 | - |
1,2-Butanediol | mutant D121N, pH 8.9, 55°C | Weizmannia coagulans | |
1.1.1.6 | 4 | - |
glycerol | wild-type, pH 8.9, 55°C | Weizmannia coagulans | |
1.1.1.6 | 4.5 | - |
D-1,2-propanediol | mutant F245S, pH 8.9, 55°C | Weizmannia coagulans | |
1.1.1.6 | 6.5 | - |
D-2,3-butanediol | mutant F245S, pH 8.9, 55°C | Weizmannia coagulans | |
1.1.1.6 | 8 | - |
D-1,2-propanediol | mutant /D121NF245S, pH 8.9, 55°C | Weizmannia coagulans | |
1.1.1.6 | 17 | - |
D-2,3-butanediol | wild-type, pH 8.9, 55°C | Weizmannia coagulans | |
1.1.1.6 | 19 | - |
D-1,2-propanediol | mutant D121N, pH 8.9, 55°C | Weizmannia coagulans | |
1.1.1.6 | 23.5 | - |
1,2-Butanediol | mutant F245S, pH 8.9, 55°C | Weizmannia coagulans | |
1.1.1.6 | 39 | - |
D-2,3-butanediol | mutant /D121NF245S, pH 8.9, 55°C | Weizmannia coagulans | |
1.1.1.6 | 41 | - |
glycerol | mutant F245S, pH 8.9, 55°C | Weizmannia coagulans | |
1.1.1.6 | 44 | - |
1,2-Butanediol | mutant /D121NF245S, pH 8.9, 55°C | Weizmannia coagulans | |
1.1.1.6 | 54.5 | - |
pyruvate | mutant F245S, pH 5.4, 55°C | Weizmannia coagulans | |
1.1.1.6 | 55.5 | - |
pyruvate | mutant /D121NF245S, pH 5.9, 55°C | Weizmannia coagulans | |
1.1.1.6 | 60.5 | - |
pyruvate | mutant D121N, pH 5.4, 55°C | Weizmannia coagulans | |
1.1.1.6 | 90 | - |
D-2,3-butanediol | mutant D121N, pH 8.9, 55°C | Weizmannia coagulans | |
1.1.1.6 | 148 | - |
glycerol | mutant /D121NF245S, pH 8.9, 55°C | Weizmannia coagulans | |
1.1.1.6 | 195 | - |
glycerol | mutant D121N, pH 8.9, 55°C | Weizmannia coagulans | |
1.1.1.6 | 207 | - |
pyruvate | wild-type, pH 5.4, 55°C | Weizmannia coagulans |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
1.1.1.6 | Zn2+ | 1.9fold increase in activity in presence of Zn2+ | Weizmannia coagulans |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.1.1.6 | 86000 | - |
gel filtration | Weizmannia coagulans |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.1.1.6 | Weizmannia coagulans | A0A150JSL8 | - |
- |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.1.1.6 | 1,2-butanediol + NAD+ | - |
Weizmannia coagulans | 1-hydroxy-2-butanone + NADH + H+ | - |
? | |
1.1.1.6 | 1,2-ethanediol + NAD+ | - |
Weizmannia coagulans | hydroxyacetaldehyde + NADH + H+ | - |
? | |
1.1.1.6 | 1,3-butanediol + NAD+ | - |
Weizmannia coagulans | 4-hydroxy-2-butanone + NADH + H+ | - |
? | |
1.1.1.6 | 1,4-butanediol + NAD+ | - |
Weizmannia coagulans | ? + NADH + H+ | - |
? | |
1.1.1.6 | D-1,2-propanediol + NAD+ | - |
Weizmannia coagulans | hydroxyacetone + NADH + H+ | - |
? | |
1.1.1.6 | D-2,3-butanediol + NAD+ | - |
Weizmannia coagulans | ? + NADH + H+ | - |
? | |
1.1.1.6 | glycerol + NAD+ | - |
Weizmannia coagulans | glycerone + NADH + H+ | - |
? | |
1.1.1.6 | L-1,2-propanediol + NAD+ | - |
Weizmannia coagulans | hydroxyacetone + NADH + H+ | - |
? | |
1.1.1.6 | L-2,3-butanediol + NAD+ | - |
Weizmannia coagulans | ? + NADH + H+ | - |
? | |
1.1.1.6 | pyruvate + NADH + H+ | - |
Weizmannia coagulans | D-lactate + NADH | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.1.1.6 | dimer | 2 * 39000, calculated from sequence | Weizmannia coagulans |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.1.1.6 | B4100_2156 | - |
Weizmannia coagulans |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.1.1.6 | 40 | 65 | mutant F245S, pH 5, reduction of pyruvate | Weizmannia coagulans |
1.1.1.6 | 60 | - |
mutant D121N/F245S | Weizmannia coagulans |
1.1.1.6 | 65 | 75 | wild-type | Weizmannia coagulans |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.1.1.6 | 70 | - |
1 h, 95% loss of activity | Weizmannia coagulans |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.1.1.6 | 1.5 | - |
pyruvate | mutant D121N, pH 5.4, 55°C | Weizmannia coagulans | |
1.1.1.6 | 10.5 | - |
D-2,3-butanediol | mutant D121N, pH 8.9, 55°C | Weizmannia coagulans | |
1.1.1.6 | 10.5 | - |
pyruvate | wild-type, pH 5.4, 55°C | Weizmannia coagulans | |
1.1.1.6 | 11 | - |
glycerol | mutant /D121NF245S, pH 8.9, 55°C | Weizmannia coagulans | |
1.1.1.6 | 29 | - |
D-1,2-propanediol | mutant D121N, pH 8.9, 55°C | Weizmannia coagulans | |
1.1.1.6 | 42.5 | - |
D-1,2-propanediol | mutant /D121NF245S, pH 8.9, 55°C | Weizmannia coagulans | |
1.1.1.6 | 42.5 | - |
glycerol | mutant F245S, pH 8.9, 55°C | Weizmannia coagulans | |
1.1.1.6 | 50 | - |
1,2-Butanediol | mutant /D121NF245S, pH 8.9, 55°C | Weizmannia coagulans | |
1.1.1.6 | 50 | - |
glycerol | mutant D121N, pH 8.9, 55°C | Weizmannia coagulans | |
1.1.1.6 | 50.5 | - |
D-2,3-butanediol | wild-type, pH 8.9, 55°C | Weizmannia coagulans | |
1.1.1.6 | 57.5 | - |
D-2,3-butanediol | mutant /D121NF245S, pH 8.9, 55°C | Weizmannia coagulans | |
1.1.1.6 | 66.5 | - |
1,2-Butanediol | mutant D121N, pH 8.9, 55°C | Weizmannia coagulans | |
1.1.1.6 | 71 | - |
1,2-Butanediol | mutant F245S, pH 8.9, 55°C | Weizmannia coagulans | |
1.1.1.6 | 87.5 | - |
D-1,2-propanediol | mutant F245S, pH 8.9, 55°C | Weizmannia coagulans | |
1.1.1.6 | 92 | - |
D-1,2-propanediol | wild-type, pH 8.9, 55°C | Weizmannia coagulans | |
1.1.1.6 | 117.5 | - |
glycerol | wild-type, pH 8.9, 55°C | Weizmannia coagulans | |
1.1.1.6 | 120.5 | - |
1,2-Butanediol | wild-type, pH 8.9, 55°C | Weizmannia coagulans | |
1.1.1.6 | 136 | - |
D-2,3-butanediol | mutant F245S, pH 8.9, 55°C | Weizmannia coagulans | |
1.1.1.6 | 165 | - |
pyruvate | mutant /D121NF245S, pH 5.9, 55°C | Weizmannia coagulans | |
1.1.1.6 | 352 | - |
pyruvate | mutant F245S, pH 5.4, 55°C | Weizmannia coagulans |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.1.1.6 | 5 | - |
mutant F245S, reduction of pyruvate | Weizmannia coagulans |
1.1.1.6 | 9 | - |
wild-type | Weizmannia coagulans |
EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.1.1.6 | 0.02 | - |
pyruvate | mutant D121N, pH 5.4, 55°C | Weizmannia coagulans | |
1.1.1.6 | 0.05 | - |
pyruvate | wild-type, pH 5.4, 55°C | Weizmannia coagulans | |
1.1.1.6 | 0.1 | - |
glycerol | mutant /D121NF245S, pH 8.9, 55°C | Weizmannia coagulans | |
1.1.1.6 | 0.1 | - |
D-2,3-butanediol | mutant D121N, pH 8.9, 55°C | Weizmannia coagulans | |
1.1.1.6 | 0.3 | - |
glycerol | mutant D121N, pH 8.9, 55°C | Weizmannia coagulans | |
1.1.1.6 | 1 | - |
1,2-Butanediol | mutant /D121NF245S, pH 8.9, 55°C | Weizmannia coagulans | |
1.1.1.6 | 1 | - |
glycerol | mutant F245S, pH 8.9, 55°C | Weizmannia coagulans | |
1.1.1.6 | 1.5 | - |
D-2,3-butanediol | mutant /D121NF245S, pH 8.9, 55°C | Weizmannia coagulans | |
1.1.1.6 | 1.5 | - |
D-1,2-propanediol | mutant D121N, pH 8.9, 55°C | Weizmannia coagulans | |
1.1.1.6 | 3 | - |
pyruvate | mutant /D121NF245S, pH 5.9, 55°C | Weizmannia coagulans | |
1.1.1.6 | 3 | - |
1,2-Butanediol | mutant F245S, pH 8.9, 55°C | Weizmannia coagulans | |
1.1.1.6 | 3 | - |
D-2,3-butanediol | wild-type, pH 8.9, 55°C | Weizmannia coagulans | |
1.1.1.6 | 5.5 | - |
D-1,2-propanediol | mutant /D121NF245S, pH 8.9, 55°C | Weizmannia coagulans | |
1.1.1.6 | 6.5 | - |
pyruvate | mutant F245S, pH 5.4, 55°C | Weizmannia coagulans | |
1.1.1.6 | 15.5 | - |
1,2-Butanediol | mutant D121N, pH 8.9, 55°C | Weizmannia coagulans | |
1.1.1.6 | 20 | - |
D-1,2-propanediol | mutant F245S, pH 8.9, 55°C | Weizmannia coagulans | |
1.1.1.6 | 21 | - |
D-2,3-butanediol | mutant F245S, pH 8.9, 55°C | Weizmannia coagulans | |
1.1.1.6 | 30 | - |
glycerol | wild-type, pH 8.9, 55°C | Weizmannia coagulans | |
1.1.1.6 | 40 | - |
1,2-Butanediol | wild-type, pH 8.9, 55°C | Weizmannia coagulans | |
1.1.1.6 | 92 | - |
D-1,2-propanediol | wild-type, pH 8.9, 55°C | Weizmannia coagulans |