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Literature summary extracted from

  • Zhang, J.; Zhao, X.; Zhang, J.; Zhao, C.; Liu, J.; Tian, Y.; Yang, L.
    Effect of deletion of 2,3-butanediol dehydrogenase gene (bdhA) on acetoin production of Bacillus subtilis (2017), Prep. Biochem. Biotechnol., 47, 761-767 .
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.1.1.4 oxygen oxygen supply in the culture of Bacillus subtilis has an important impact on the product yield, productivity and 2,3-butanediol formation in acetoin fermentation. In general, high oxygen supply favours acetoin formation and decrease 2,3-butanediol final yield Bacillus subtilis
1.1.1.B20 oxygen oxygen supply in the culture of Bacillus subtilis has an important impact on the product yield, productivity and 2,3-butanediol formation in acetoin fermentation. In general, high oxygen supply favours acetoin formation and decrease 2,3-butanediol final yield Bacillus subtilis

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.4 gene bdhA, the the BDH gene sequence is cloned from genomic DNA from strain 168, the BDH encoded by the gene bdhA is responsible for catalysing the reaction from (3R)-acetoin to (2R,3R)-2,3-butanediol Bacillus subtilis
1.1.1.B20 gene bdhA, the the BDH gene sequence is cloned from genomic DNA from strain 168, the BDH encoded by the gene bdhA is responsible for catalysing the reaction from (3R)-acetoin to (2R,3R)-2,3-butanediol Bacillus subtilis

Protein Variants

EC Number Protein Variants Comment Organism
1.1.1.4 additional information construction of an engineered Bacillus subtilis strain 168 in which the bdhA gene is knocked out by the cre/lox system using the lox71-zeo-lox66 resistance marker cassette. The effects of bdhA gene deletion on production of acetoin and 2,3-butanediol are evaluated. By increasing the glucose concentration, the acetoin yield is improved from 6.61 g/l to 24.6 g/l. Deletion of the gene bdhA efficiently blocks the transformation of acetoin and 2,3-butanediol during the fermentation of strain BS168D, overview Bacillus subtilis
1.1.1.B20 additional information construction of an engineered Bacillus subtilis strain 168 in which the bdhA gene is knocked out by the cre/lox system using the lox71-zeo-lox66 resistance marker cassette. The effects of bdhA gene deletion on production of acetoin and 2,3-butanediol are evaluated. By increasing the glucose concentration, the acetoin yield is improved from 6.61 g/l to 24.6 g/l. Deletion of the gene bdhA efficiently blocks the transformation of acetoin and 2,3-butanediol during the fermentation of strain BS168D, overview Bacillus subtilis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.4 (R,R)-butane-2,3-diol + NAD+ Bacillus subtilis
-
(R)-acetoin + NADH + H+
-
r
1.1.1.4 (R,R)-butane-2,3-diol + NAD+ Bacillus subtilis BS168D
-
(R)-acetoin + NADH + H+
-
r
1.1.1.B20 (2R,3S)-butane-2,3-diol + NAD+ Bacillus subtilis
-
(3R)-acetoin + NADH + H+
-
r
1.1.1.B20 (2R,3S)-butane-2,3-diol + NAD+ Bacillus subtilis BS168D
-
(3R)-acetoin + NADH + H+
-
r

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.4 Bacillus subtilis O34788
-
-
1.1.1.4 Bacillus subtilis BS168D O34788
-
-
1.1.1.B20 Bacillus subtilis O34788
-
-
1.1.1.B20 Bacillus subtilis BS168D O34788
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.1.1.4 additional information cultivation of wild-type and mutant strains at pH 7.0, 37°C Bacillus subtilis
-
1.1.1.B20 additional information cultivation of wild-type and mutant strains at pH 7.0, 37°C Bacillus subtilis
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.4 (R,R)-butane-2,3-diol + NAD+
-
Bacillus subtilis (R)-acetoin + NADH + H+
-
r
1.1.1.4 (R,R)-butane-2,3-diol + NAD+
-
Bacillus subtilis BS168D (R)-acetoin + NADH + H+
-
r
1.1.1.B20 (2R,3S)-butane-2,3-diol + NAD+
-
Bacillus subtilis (3R)-acetoin + NADH + H+
-
r
1.1.1.B20 (2R,3S)-butane-2,3-diol + NAD+
-
Bacillus subtilis BS168D (3R)-acetoin + NADH + H+
-
r

Synonyms

EC Number Synonyms Comment Organism
1.1.1.4 2,3-butanediol dehydrogenase
-
Bacillus subtilis
1.1.1.4 BDH
-
Bacillus subtilis
1.1.1.4 BdhA
-
Bacillus subtilis
1.1.1.B20 2,3-butanediol dehydrogenase
-
Bacillus subtilis
1.1.1.B20 BDH
-
Bacillus subtilis
1.1.1.B20 BdhA
-
Bacillus subtilis

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.4 NAD+
-
Bacillus subtilis
1.1.1.4 NADH
-
Bacillus subtilis
1.1.1.B20 NAD+
-
Bacillus subtilis
1.1.1.B20 NADH
-
Bacillus subtilis

General Information

EC Number General Information Comment Organism
1.1.1.4 malfunction deletion of bdhA gene successfully blocks the reversible transformation between acetoin and 2,3-butanediol and eliminates the effect of dissolved oxygen on the transformation Bacillus subtilis
1.1.1.4 physiological function acetoin and 2,3-butanediol can be transformed into each other by 2,3-butanediol dehydrogenase (BDH) using NADH/NAD+ as coenzyme. The main 2,3-butanediol production of strain BS168D is meso-2,3-butanediol and the bdhA gene is only responsible for (2R,3R)-2,3-butanediol synthesis. Oxygen supply in the culture of Bacillus subtilis has an important impact on the product yield, productivity and 2,3-butanediol formation in acetoin fermentation. In general, high oxygen supply favours acetoin formation and decrease 2,3-butanediol final yield Bacillus subtilis
1.1.1.B20 malfunction deletion of bdhA gene successfully blocks the reversible transformation between acetoin and 2,3-butanediol and eliminates the effect of dissolved oxygen on the transformation Bacillus subtilis
1.1.1.B20 physiological function acetoin and 2,3-butanediol can be transformed into each other by 2,3-butanediol dehydrogenase (BDH) using NADH/NAD+ as coenzyme. The main 2,3-butanediol production of strain BS168D is meso-2,3-butanediol and the bdhA gene is only responsible for (2R,3R)-2,3-butanediol synthesis. Oxygen supply in the culture of Bacillus subtilis has an important impact on the product yield, productivity and 2,3-butanediol formation in acetoin fermentation. In general, high oxygen supply favours acetoin formation and decrease 2,3-butanediol final yield Bacillus subtilis