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Literature summary extracted from

  • Yamada, R.; Wakita, K.; Mitsui, R.; Nishikawa, R.; Ogino, H.
    Efficient production of 2,3-butanediol by recombinant Saccharomyces cerevisiae through modulation of gene expression by cocktail delta-integration (2017), Biores. Technol., 245, 1558-1566 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.4 recombinant enzyme expression in Saccharomyces cerevisiae strain YPH499, co-expression with alpha-acetolactate synthase from Bacillus subtilis, alpha-acetolactate decarboxylase from Bacillus amyloliquefaciens, and NADH oxidase from Lactococcus lactis Bacillus amyloliquefaciens

Protein Variants

EC Number Protein Variants Comment Organism
1.1.1.4 additional information expression of 4 genes encoding alpha-acetolactate synthase from Bacillus subtilis, alpha-acetolactate decarboxylase and 2,3-butanediol dehydrogenase from Bacillus amyloliquefaciens, and NADH oxidase from Lactococcus lactis in Saccharomyces cerevisiae strain YPH499 is modulated using a cocktail delta-integration strategy. The resultant strain, YPH499/dPdAdG/BD6-10, is used in a fed-batch cultivation for the production of 2,3-butanediol. The concentration, production rate, and yield obtained are 80.0 g/l, 4.00 g/l/h, and 41.7%, respectively. The cocktail delta-integration strategy leads to the upregulation of BsAlsS and BaAlsD genes in the modified strain, which results in higher production rate of 2,3-butanediol. The conversion rate of pyruvate to alpha-acetolactate, catalyzed by BsAlsS, and of alpha-acetolactate into acetoin (catalyzed by BaAlsD) must be slower compared to that of acetoin being converted to 2,3-butanediol catalyzed by BaBdhA in the control strain. The conversion of alpha-acetolactate into acetoin catalyzed by alpha-acetolactate decarboxylase might be the rate limiting step in 2,3-butanediol production in recombinant Saccharomyces cerevisiae. Method development and evaluation, overview Bacillus amyloliquefaciens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.4 (R,R)-butane-2,3-diol + NAD+ Bacillus amyloliquefaciens
-
 (R)-acetoin + NADH + H+
-
 r
1.1.1.4 (R,R)-butane-2,3-diol + NAD+ Bacillus amyloliquefaciens UCMB5033
-
 (R)-acetoin + NADH + H+
-
 r

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.4 Bacillus amyloliquefaciens S6FPW0 Bacillus velezensis
-
1.1.1.4 Bacillus amyloliquefaciens UCMB5033 S6FPW0 Bacillus velezensis
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.1.1.4 additional information highest 2,3-butanediol (BDO) production rate and yield are achieved in fed-batch cultivation Bacillus amyloliquefaciens
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.4 (R,R)-butane-2,3-diol + NAD+
-
 Bacillus amyloliquefaciens (R)-acetoin + NADH + H+
-
 r
1.1.1.4 (R,R)-butane-2,3-diol + NAD+
-
 Bacillus amyloliquefaciens UCMB5033 (R)-acetoin + NADH + H+
-
 r

Synonyms

EC Number Synonyms Comment Organism
1.1.1.4 2,3-butanediol dehydrogenase
-
 Bacillus amyloliquefaciens
1.1.1.4 BaBdhA
-
 Bacillus amyloliquefaciens
1.1.1.4 BDH
-
 Bacillus amyloliquefaciens
1.1.1.4 BdhA
-
 Bacillus amyloliquefaciens

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.4 NAD+
-
 Bacillus amyloliquefaciens
1.1.1.4 NADH
-
 Bacillus amyloliquefaciens