Any feedback?
Literature summary extracted from
- Cloning, expression and characterization of a novel (2R,3R) -2,3-butanediol dehydrogenase from Bacillus thuringiensis (2019), Biocatal. Agricult. Biotechnol., 22, 10137 .
No PubMed abstract available
Activating Compound
| EC Number | Activating Compound | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.1.1.4 | additional information | nonionic detergent Triton X-100 and Tween 80, as well as DMSO have no significant effect on enzyme activity | Bacillus thuringiensis serovar kurstaki | |
| 1.1.1.B20 | additional information | nonionic detergent Triton X-100 and Tween 80, as well as DMSO have no significant effect on enzyme activity | Bacillus thuringiensis serovar kurstaki |
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 1.1.1.4 | gene bdh, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, recombinant expression in Escherichia coli | Bacillus thuringiensis serovar kurstaki |
| 1.1.1.B20 | gene bdh, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, recombinant expression in Escherichia coli | Bacillus thuringiensis serovar kurstaki |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.1.1.4 | EDTA | - |
Bacillus thuringiensis serovar kurstaki |  |
| 1.1.1.4 | additional information | nonionic detergent Triton X-100 and Tween 80, as well as DMSO have no significant effect on enzyme activity | Bacillus thuringiensis serovar kurstaki | |
| 1.1.1.B20 | EDTA | - |
Bacillus thuringiensis serovar kurstaki | |
| 1.1.1.B20 | additional information | nonionic detergent Triton X-100 and Tween 80, as well as DMSO have no significant effect on enzyme activity | Bacillus thuringiensis serovar kurstaki |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.1.1.4 | 0.76 | - |
(2R,3R)-butane-2,3-diol | pH 10.0, 50°C, recombinant enzyme | Bacillus thuringiensis serovar kurstaki | |
| 1.1.1.4 | 3.67 | - |
(2R,3S)-butane-2,3-diol | pH 10.0, 50°C, recombinant enzyme | Bacillus thuringiensis serovar kurstaki | |
| 1.1.1.B20 | 0.76 | - |
(2R,3R)-butane-2,3-diol | pH 10.0, 50°C, recombinant enzyme | Bacillus thuringiensis serovar kurstaki | |
| 1.1.1.B20 | 3.67 | - |
(2R,3S)-butane-2,3-diol | pH 10.0, 50°C, recombinant enzyme | Bacillus thuringiensis serovar kurstaki | |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.1.1.4 | Zn2+ | required, the enzyme has a catalytic Zn site (Cys37, His69 and Glu151) and a structural Zn site (Cys99, Cys102, Cys105 and Cys113) | Bacillus thuringiensis serovar kurstaki | |
| 1.1.1.B20 | Zn2+ | required, the enzyme has a catalytic Zn site (Cys37, His69 and Glu151) and a structural Zn site (Cys99, Cys102, Cys105 and Cys113) | Bacillus thuringiensis serovar kurstaki | |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.1.1.4 | (2R,3R)-butane-2,3-diol + NAD+ | Bacillus thuringiensis serovar kurstaki | - |
(3R)-acetoin + NADH + H+ | - |
r | |
| 1.1.1.4 | (2R,3R)-butane-2,3-diol + NAD+ | Bacillus thuringiensis serovar kurstaki ACCC 10066 | - |
(3R)-acetoin + NADH + H+ | - |
r | |
| 1.1.1.4 | (2R,3S)-butane-2,3-diol + NAD+ | Bacillus thuringiensis serovar kurstaki | - |
(3R)-acetoin + NADH + H+ | - |
r | |
| 1.1.1.4 | (2R,3S)-butane-2,3-diol + NAD+ | Bacillus thuringiensis serovar kurstaki ACCC 10066 | - |
(3R)-acetoin + NADH + H+ | - |
r | |
| 1.1.1.B20 | (2R,3R)-butane-2,3-diol + NAD+ | Bacillus thuringiensis serovar kurstaki | - |
(3R)-acetoin + NADH + H+ | - |
r | |
| 1.1.1.B20 | (2R,3S)-butane-2,3-diol + NAD+ | Bacillus thuringiensis serovar kurstaki | - |
(3R)-acetoin + NADH + H+ | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.1.1.4 | Bacillus thuringiensis serovar kurstaki | A0A0F6FX23 | - |
- |
| 1.1.1.4 | Bacillus thuringiensis serovar kurstaki ACCC 10066 | A0A0F6FX23 | - |
- |
| 1.1.1.B20 | Bacillus thuringiensis serovar kurstaki | A0A0F6FX23 | - |
- |
| 1.1.1.B20 | Bacillus thuringiensis serovar kurstaki ACCC 10066 | A0A0F6FX23 | - |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 1.1.1.4 | recombinant enzyme from Escherichia coli by ion exchange chromatography | Bacillus thuringiensis serovar kurstaki |
| 1.1.1.B20 | recombinant enzyme from Escherichia coli by ion exchange chromatography | Bacillus thuringiensis serovar kurstaki |
Storage Stability
| EC Number | Storage Stability | Organism |
|---|---|---|
| 1.1.1.4 | 4°C, BtBDH shows good stability after storage for 3 months | Bacillus thuringiensis serovar kurstaki |
| 1.1.1.B20 | 4°C, BtBDH shows good stability after storage for 3 months | Bacillus thuringiensis serovar kurstaki |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.1.1.4 | (2R,3R)-butane-2,3-diol + NAD+ | - |
Bacillus thuringiensis serovar kurstaki | (3R)-acetoin + NADH + H+ | - |
r | |
| 1.1.1.4 | (2R,3R)-butane-2,3-diol + NAD+ | - |
Bacillus thuringiensis serovar kurstaki ACCC 10066 | (3R)-acetoin + NADH + H+ | - |
r | |
| 1.1.1.4 | (2R,3S)-butane-2,3-diol + NAD+ | - |
Bacillus thuringiensis serovar kurstaki | (3R)-acetoin + NADH + H+ | - |
r | |
| 1.1.1.4 | (2R,3S)-butane-2,3-diol + NAD+ | slightly preferred substrate | Bacillus thuringiensis serovar kurstaki | (3R)-acetoin + NADH + H+ | - |
r | |
| 1.1.1.4 | (2R,3S)-butane-2,3-diol + NAD+ | - |
Bacillus thuringiensis serovar kurstaki ACCC 10066 | (3R)-acetoin + NADH + H+ | - |
r | |
| 1.1.1.4 | (2R,3S)-butane-2,3-diol + NAD+ | slightly preferred substrate | Bacillus thuringiensis serovar kurstaki ACCC 10066 | (3R)-acetoin + NADH + H+ | - |
r | |
| 1.1.1.4 | additional information | enzyme BtBDH is active with meso-2,3-butanediol and (2R,3R)-2,3-butanediol, whereas no activity is observed with (2S,3S)-2,3-butanediol. BtBDH shows similar oxidative activity toward meso-2,3-butanediol and (2R,3R)-2,3-butanediol, and it exhibits a 3fold higher reduction activity toward acetoin compared to diacetyl | Bacillus thuringiensis serovar kurstaki | ? | - |
- |
|
| 1.1.1.4 | additional information | enzyme BtBDH is active with meso-2,3-butanediol and (2R,3R)-2,3-butanediol, whereas no activity is observed with (2S,3S)-2,3-butanediol. BtBDH shows similar oxidative activity toward meso-2,3-butanediol and (2R,3R)-2,3-butanediol, and it exhibits a 3fold higher reduction activity toward acetoin compared to diacetyl | Bacillus thuringiensis serovar kurstaki ACCC 10066 | ? | - |
- |
|
| 1.1.1.B20 | (2R,3R)-butane-2,3-diol + NAD+ | - |
Bacillus thuringiensis serovar kurstaki | (3R)-acetoin + NADH + H+ | - |
r | |
| 1.1.1.B20 | (2R,3S)-butane-2,3-diol + NAD+ | - |
Bacillus thuringiensis serovar kurstaki | (3R)-acetoin + NADH + H+ | - |
? | |
| 1.1.1.B20 | (2R,3S)-butane-2,3-diol + NAD+ | slightly preferred substrate | Bacillus thuringiensis serovar kurstaki | (3R)-acetoin + NADH + H+ | - |
? | |
| 1.1.1.B20 | additional information | enzyme BtBDH is active with meso-2,3-butanediol and (2R,3R)-2,3-butanediol, whereas no activity is observed with (2S,3S)-2,3-butanediol. BtBDH shows similar oxidative activity toward meso-2,3-butanediol and (2R,3R)-2,3-butanediol, and it exhibits a 3fold higher reduction activity toward acetoin compared to diacetyl | Bacillus thuringiensis serovar kurstaki | ? | - |
- |
|
| 1.1.1.B20 | additional information | enzyme BtBDH is active with meso-2,3-butanediol and (2R,3R)-2,3-butanediol, whereas no activity is observed with (2S,3S)-2,3-butanediol. BtBDH shows similar oxidative activity toward meso-2,3-butanediol and (2R,3R)-2,3-butanediol, and it exhibits a 3fold higher reduction activity toward acetoin compared to diacetyl | Bacillus thuringiensis serovar kurstaki ACCC 10066 | ? | - |
- |
|
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 1.1.1.4 | ? | x * 37800, about, sequence calculation, x * 40000, recombinant enzyme, SDS-PAGE | Bacillus thuringiensis serovar kurstaki |
| 1.1.1.B20 | ? | x * 37800, about, sequence calculation, x * 40000, recombinant enzyme, SDS-PAGE | Bacillus thuringiensis serovar kurstaki |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 1.1.1.4 | (2R,3R)-2,3-butanediol dehydrogenase | - |
Bacillus thuringiensis serovar kurstaki |
| 1.1.1.4 | BtBDH | - |
Bacillus thuringiensis serovar kurstaki |
| 1.1.1.4 | butanediol dehydrogenase | - |
Bacillus thuringiensis serovar kurstaki |
| 1.1.1.B20 | (2R,3R)-2,3-butanediol dehydrogenase | - |
Bacillus thuringiensis serovar kurstaki |
| 1.1.1.B20 | BtBDH | - |
Bacillus thuringiensis serovar kurstaki |
| 1.1.1.B20 | butanediol dehydrogenase | - |
Bacillus thuringiensis serovar kurstaki |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.1.1.4 | 35 | - |
reduction activity | Bacillus thuringiensis serovar kurstaki |
| 1.1.1.4 | 50 | - |
oxidation activity | Bacillus thuringiensis serovar kurstaki |
| 1.1.1.B20 | 35 | - |
reduction activity | Bacillus thuringiensis serovar kurstaki |
| 1.1.1.B20 | 50 | - |
oxidation activity | Bacillus thuringiensis serovar kurstaki |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.1.1.4 | 70 | - |
stable up to | Bacillus thuringiensis serovar kurstaki |
| 1.1.1.B20 | 70 | - |
stable up to | Bacillus thuringiensis serovar kurstaki |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.1.1.4 | 7.5 | - |
reduction activity | Bacillus thuringiensis serovar kurstaki |
| 1.1.1.4 | 10 | - |
oxidation activity | Bacillus thuringiensis serovar kurstaki |
| 1.1.1.B20 | 7.5 | - |
reduction activity | Bacillus thuringiensis serovar kurstaki |
| 1.1.1.B20 | 10 | - |
oxidation activity | Bacillus thuringiensis serovar kurstaki |
pH Stability
| EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.1.1.4 | 6 | 10 | stable at | Bacillus thuringiensis serovar kurstaki |
| 1.1.1.B20 | 6 | 10 | stable at | Bacillus thuringiensis serovar kurstaki |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.1.1.4 | NAD+ | - |
Bacillus thuringiensis serovar kurstaki | |
| 1.1.1.4 | NADH | - |
Bacillus thuringiensis serovar kurstaki | |
| 1.1.1.B20 | NAD+ | - |
Bacillus thuringiensis serovar kurstaki | |
| 1.1.1.B20 | NADH | - |
Bacillus thuringiensis serovar kurstaki | |
pI Value
| EC Number | Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|---|
| 1.1.1.4 | Bacillus thuringiensis serovar kurstaki | sequence calculation | - |
5.5 |
| 1.1.1.B20 | Bacillus thuringiensis serovar kurstaki | sequence calculation | - |
5.5 |
General Information
| EC Number | General Information | Comment | Organism |
|---|---|---|---|
| 1.1.1.4 | evolution | enzyme BtBDH contains a GroES-like domain at the N terminus and a NAD(P)-binding domain at the C-terminus. Phylogenetic tree analysis reveals that BtBDH is a member ofthe (2R,3R)-2,3-BDH group. BtBDH has the typical (2R,3R)-2,3-butanediol dehydrogenase properties and belongs to the MDR superfamily. According to previous reports, (2R,3R)-2,3-BDH generally belongs to the MDR family, while meso-2,3-BDH is commonly clustered in the SDR (short chain dehydrogenase/reductase) family | Bacillus thuringiensis serovar kurstaki |
| 1.1.1.B20 | evolution | enzyme BtBDH contains a GroES-like domain at the N terminus and a NAD(P)-binding domain at the C-terminus. Phylogenetic tree analysis reveals that BtBDH is a member ofthe (2R,3R)-2,3-BDH group. BtBDH has the typical (2R,3R)-2,3-butanediol dehydrogenase properties and belongs to the MDR superfamily. According to previous reports, (2R,3R)-2,3-BDH generally belongs to the MDR family, while meso-2,3-BDH is commonly clustered in the SDR (short chain dehydrogenase/reductase) family | Bacillus thuringiensis serovar kurstaki |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
