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Literature summary extracted from

  • Kuang, X.; Ouyang, Y.; Guo, Y.; Li, Q.; Wang, H.; Abrha, G.T.; Ayepa, E.; Gu, Y.; Li, X.; Chen, Q.; Ma, M.
    New insights into two yeast BDHs from the PDH subfamily as aldehyde reductases in context of detoxification of lignocellulosic aldehyde inhibitors (2020), Appl. Microbiol. Biotechnol., 104, 6679-6692 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.4 gene BDH1, cloning from Saccharomyces cerevisiae strains YBA_08 (denoted Y) and BY4742 (denoted B), DNA and amino acid sequence determination and analysis and phylogenetic tree analysis, subcloning in to Escherichia coli strain DH5alpha and functional overexpression in Saccharomyces cerevisiae strain INVSc1, recombinant expression of His-tagged enzymes originnating from both strains Saccharomyces cerevisiae

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.4 0.65
-
 acetaldehyde recombinant enzyme, pH 7.0, 30°C, with NADH, from strain B Saccharomyces cerevisiae
1.1.1.4 0.67
-
 acetaldehyde recombinant enzyme, pH 7.0, 30°C, with NADH, from strain B Saccharomyces cerevisiae
1.1.1.4 4.49
-
 acetaldehyde recombinant enzyme, pH 7.0, 30°C, with NADH, from strain Y Saccharomyces cerevisiae
1.1.1.4 9.38
-
 glycolaldehyde recombinant enzyme, pH 7.0, 30°C, with NADH, from strain Y Saccharomyces cerevisiae
1.1.1.4 15.52
-
 glycolaldehyde recombinant enzyme, pH 7.0, 30°C, with NADH, from strain Y Saccharomyces cerevisiae
1.1.1.4 17.91
-
 glycolaldehyde recombinant enzyme, pH 7.0, 30°C, with NADH, from strain B Saccharomyces cerevisiae
1.1.1.4 20.18
-
 acetaldehyde recombinant enzyme, pH 7.0, 30°C, with NADH, from strain Y Saccharomyces cerevisiae
1.1.1.4 29.42
-
 glycolaldehyde recombinant enzyme, pH 7.0, 30°C, with NADH, from strain B Saccharomyces cerevisiae

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.1.1.4 Zn2+ the enzyme has a catalytic zinc binding domain Saccharomyces cerevisiae

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.4 (R,R)-butane-2,3-diol + NAD+ Saccharomyces cerevisiae
-
 (R)-acetoin + NADH + H+
-
 ?
1.1.1.4 (R,R)-butane-2,3-diol + NAD+ Saccharomyces cerevisiae YBA_08
-
 (R)-acetoin + NADH + H+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.4 Saccharomyces cerevisiae A0A7G4NVP1 strain Y/YBA_08
-
1.1.1.4 Saccharomyces cerevisiae A0A7G4NVP2 strain Y/YBA_08
-
1.1.1.4 Saccharomyces cerevisiae YBA_08 A0A7G4NVP1 strain Y/YBA_08
-
1.1.1.4 Saccharomyces cerevisiae YBA_08 A0A7G4NVP2 strain Y/YBA_08
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.4 recombinant His-tagged enzyme by nickel affinity chromatography Saccharomyces cerevisiae

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.4 0.24
-
 purified recombinant enzyme, pH 7.0, 30°C, substrates glycolaldehyde and NADPH Saccharomyces cerevisiae
1.1.1.4 0.48
-
 purified recombinant enzyme, pH 7.0, 30°C, substrates formaldehyde and NADPH Saccharomyces cerevisiae
1.1.1.4 0.76
-
 purified recombinant enzyme, pH 7.0, 30°C, substrates formaldehyde and NADPH Saccharomyces cerevisiae
1.1.1.4 0.82
-
 purified recombinant enzyme, pH 7.0, 30°C, substrates furfural and NADPH Saccharomyces cerevisiae
1.1.1.4 1.16
-
 purified recombinant enzyme, pH 7.0, 30°C, substrates hexaldehyde and NADH Saccharomyces cerevisiae
1.1.1.4 1.3
-
 purified recombinant enzyme, pH 7.0, 30°C, substrates formaldehyde and NADH Saccharomyces cerevisiae
1.1.1.4 1.71
-
 purified recombinant enzyme, pH 7.0, 30°C, substrates formaldehyde and NADH Saccharomyces cerevisiae
1.1.1.4 1.94
-
 purified recombinant enzyme, pH 7.0, 30°C, substrates propionaldehyde and NADH Saccharomyces cerevisiae
1.1.1.4 2.47
-
 purified recombinant enzyme, pH 7.0, 30°C, substrates hexaldehyde and NADH Saccharomyces cerevisiae
1.1.1.4 2.72
-
 purified recombinant enzyme, pH 7.0, 30°C, substrates acetaldehyde and NADPH Saccharomyces cerevisiae
1.1.1.4 2.74
-
 purified recombinant enzyme, pH 7.0, 30°C, substrates furfural and NADH Saccharomyces cerevisiae
1.1.1.4 3.44
-
 purified recombinant enzyme, pH 7.0, 30°C, substrates acetaldehyde and NADPH Saccharomyces cerevisiae
1.1.1.4 4.95
-
 purified recombinant enzyme, pH 7.0, 30°C, substrates furfural and NADH Saccharomyces cerevisiae
1.1.1.4 6.27
-
 purified recombinant enzyme, pH 7.0, 30°C, substrates glycolaldehyde and NADH Saccharomyces cerevisiae
1.1.1.4 10.2
-
 purified recombinant enzyme, pH 7.0, 30°C, substrates glycolaldehyde and NADH Saccharomyces cerevisiae
1.1.1.4 78.58
-
 purified recombinant enzyme, pH 7.0, 30°C, substrates acetaldehyde and NADH Saccharomyces cerevisiae
1.1.1.4 117.95
-
 purified recombinant enzyme, pH 7.0, 30°C, substrates acetaldehyde and NADH Saccharomyces cerevisiae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.4 (R,R)-butane-2,3-diol + NAD+
-
 Saccharomyces cerevisiae (R)-acetoin + NADH + H+
-
 ?
1.1.1.4 (R,R)-butane-2,3-diol + NAD+
-
 Saccharomyces cerevisiae YBA_08 (R)-acetoin + NADH + H+
-
 ?
1.1.1.4 acetaldehyde + NADH + H+
-
 Saccharomyces cerevisiae ethanol + NAD+
-
 ?
1.1.1.4 acetaldehyde + NADH + H+
-
 Saccharomyces cerevisiae YBA_08 ethanol + NAD+
-
 ?
1.1.1.4 acetaldehyde + NADPH + H+
-
 Saccharomyces cerevisiae ethanol + NADP+
-
 ?
1.1.1.4 acetaldehyde + NADPH + H+
-
 Saccharomyces cerevisiae YBA_08 ethanol + NADP+
-
 ?
1.1.1.4 formaldehyde + NADH + H+
-
 Saccharomyces cerevisiae ? + NAD+
-
 ?
1.1.1.4 formaldehyde + NADPH + H+
-
 Saccharomyces cerevisiae ? + NADP+
-
 ?
1.1.1.4 furfural + NADH + H+
-
 Saccharomyces cerevisiae (furan-2-yl)methanol + NAD+
-
 ?
1.1.1.4 furfural + NADH + H+
-
 Saccharomyces cerevisiae YBA_08 (furan-2-yl)methanol + NAD+
-
 ?
1.1.1.4 furfural + NADPH + H+
-
 Saccharomyces cerevisiae (furan-2-yl)methanol + NADP+
-
 ?
1.1.1.4 glycolaldehyde + NADH + H+
-
 Saccharomyces cerevisiae ? + NAD+
-
 ?
1.1.1.4 hexaldehyde + NADH + H+
-
 Saccharomyces cerevisiae ? + NAD+
-
 ?
1.1.1.4 additional information enzyme Bdh1p exhibits (2R,3R)-2,3-butanediol dehydrogenase activity, but Bdh1p also exhibit reductive activities towards lignocellulosic aldehyde inhibitors, such as acetaldehyde, glycolaldehyde, and furfural. No activity for glycolaldehyde or hexaldehyde with NADPH. Propionaldehyde is a poor substrate, no activity with glutaraldehyde Saccharomyces cerevisiae ?
-
-
1.1.1.4 additional information enzyme Bdh1p exhibits (2R,3R)-2,3-butanediol dehydrogenase activity, but Bdh2p also exhibit reductive activities towards lignocellulosic aldehyde inhibitors, such as acetaldehyde, glycolaldehyde, and furfural. No activity for furfural, propionaldehyde, or hexaldehyde with NADPH. Glutaraldehyde is a poor substrate Saccharomyces cerevisiae ?
-
-
1.1.1.4 additional information enzyme Bdh1p exhibits (2R,3R)-2,3-butanediol dehydrogenase activity, but Bdh1p also exhibit reductive activities towards lignocellulosic aldehyde inhibitors, such as acetaldehyde, glycolaldehyde, and furfural. No activity for glycolaldehyde or hexaldehyde with NADPH. Propionaldehyde is a poor substrate, no activity with glutaraldehyde Saccharomyces cerevisiae YBA_08 ?
-
-
1.1.1.4 additional information enzyme Bdh1p exhibits (2R,3R)-2,3-butanediol dehydrogenase activity, but Bdh2p also exhibit reductive activities towards lignocellulosic aldehyde inhibitors, such as acetaldehyde, glycolaldehyde, and furfural. No activity for furfural, propionaldehyde, or hexaldehyde with NADPH. Glutaraldehyde is a poor substrate Saccharomyces cerevisiae YBA_08 ?
-
-
1.1.1.4 propionaldehyde + NADH + H+
-
 Saccharomyces cerevisiae ? + NAD+
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.1.1.4 (2R,3R)-2,3-butanediol dehydrogenase
-
 Saccharomyces cerevisiae
1.1.1.4 BDH
-
 Saccharomyces cerevisiae
1.1.1.4 BDH1
-
 Saccharomyces cerevisiae
1.1.1.4 Bdh1p
-
 Saccharomyces cerevisiae
1.1.1.4 BDH2
-
 Saccharomyces cerevisiae
1.1.1.4 Bdh2p
-
 Saccharomyces cerevisiae

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.1.4 35 45 aldehyde reduction activity Saccharomyces cerevisiae

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.1.1.4 7.39
-
 glycolaldehyde recombinant enzyme, pH 7.0, 30°C, with NADH, from strain B Saccharomyces cerevisiae
1.1.1.4 9.38
-
 acetaldehyde recombinant enzyme, pH 7.0, 30°C, with NADH, from strain B Saccharomyces cerevisiae
1.1.1.4 9.43
-
 glycolaldehyde recombinant enzyme, pH 7.0, 30°C, with NADH, from strain B Saccharomyces cerevisiae
1.1.1.4 10.03
-
 acetaldehyde recombinant enzyme, pH 7.0, 30°C, with NADH, from strain B Saccharomyces cerevisiae
1.1.1.4 37.32
-
 glycolaldehyde recombinant enzyme, pH 7.0, 30°C, with NADH, from strain Y Saccharomyces cerevisiae
1.1.1.4 53.46
-
 glycolaldehyde recombinant enzyme, pH 7.0, 30°C, with NADH, from strain Y Saccharomyces cerevisiae
1.1.1.4 56.57
-
 acetaldehyde recombinant enzyme, pH 7.0, 30°C, with NADH, from strain Y Saccharomyces cerevisiae
1.1.1.4 96.62
-
 acetaldehyde recombinant enzyme, pH 7.0, 30°C, with NADH, from strain Y Saccharomyces cerevisiae

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.4 6.5 7.5 aldehyde reduction activity Saccharomyces cerevisiae

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.1.1.4 4.5 9 activities of Bdh1p(Y) and Bdh1p(B) in glucoladehyde reduction gradually drop to approximately 50% at pH 4.5-7.0 after 6 h incubation. Especially, Bdh1p(Y) still retains 80% of its activity at pH 4.5 after incubation for 3 h. At alkaline conditions (pH 8.0-9.0), activities of the two enzymes drop rapidly to 28-45% within the first 3 h, and 3 more hours later, the lowest activity of Bdh1p(Y) is 17% at pH 9.0 Saccharomyces cerevisiae

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.4 NAD+
-
 Saccharomyces cerevisiae
1.1.1.4 NADH
-
 Saccharomyces cerevisiae
1.1.1.4 NADPH
-
 Saccharomyces cerevisiae

General Information

EC Number General Information Comment Organism
1.1.1.4 evolution two (2R,3R)-2,3-butanediol dehydrogenases (BDHs) from industrial (denoted Y)/laboratory (denoted B) strains of Saccharomyces cerevisiae, Bdh1p(Y)/Bdh1p(B) and Bdh2p(Y)/Bdh2p(B), are members of the PDH subfamily with an NAD(P)H binding domain and a catalytic zinc binding domain, and exhibit reductive activities towards lignocellulosic aldehyde inhibitors, such as acetaldehyde, glycolaldehyde, and furfural Saccharomyces cerevisiae

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.1.1.4 0.32
-
 glycolaldehyde recombinant enzyme, pH 7.0, 30°C, with NADH, from strain B Saccharomyces cerevisiae
1.1.1.4 0.41
-
 glycolaldehyde recombinant enzyme, pH 7.0, 30°C, with NADH, from strain B Saccharomyces cerevisiae
1.1.1.4 2.41
-
 glycolaldehyde recombinant enzyme, pH 7.0, 30°C, with NADH, from strain Y Saccharomyces cerevisiae
1.1.1.4 4.79
-
 acetaldehyde recombinant enzyme, pH 7.0, 30°C, with NADH, from strain Y Saccharomyces cerevisiae
1.1.1.4 5.7
-
 glycolaldehyde recombinant enzyme, pH 7.0, 30°C, with NADH, from strain Y Saccharomyces cerevisiae
1.1.1.4 12.6
-
 acetaldehyde recombinant enzyme, pH 7.0, 30°C, with NADH, from strain Y Saccharomyces cerevisiae
1.1.1.4 14
-
 acetaldehyde recombinant enzyme, pH 7.0, 30°C, with NADH, from strain B Saccharomyces cerevisiae
1.1.1.4 15.43
-
 acetaldehyde recombinant enzyme, pH 7.0, 30°C, with NADH, from strain B Saccharomyces cerevisiae