EC Number | Cloned (Comment) | Organism |
---|---|---|
1.1.1.4 | genetic structure of the 2,3-BD utilization operon in Pseudomonas aeruginosa strain PAO1 and comparisons, overview | Pseudomonas aeruginosa |
1.1.1.B20 | genetic structure of the 2,3-BD utilization operon in Pseudomonas aeruginosa strain PAO1 and comparisons, overview | Pseudomonas aeruginosa |
1.1.1.76 | genetic structure of the 2,3-BD utilization operon in Pseudomonas aeruginosa strain PAO1 and comparisons, overview | Pseudomonas aeruginosa |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.1.1.4 | 0.057 | - |
(2R,3R)-butane-2,3-diol | pH 7.4, 30°C | Pseudomonas aeruginosa | |
1.1.1.B20 | 0.128 | - |
(2R,3S)-butane-2,3-diol | pH 7.4, 30°C | Pseudomonas aeruginosa | |
1.1.1.76 | 0.024 | - |
(2S,3S)-butane-2,3-diol | pH 7.4, 30°C | Pseudomonas aeruginosa | |
2.3.1.190 | 0.055 | - |
acetoin | pH 7.4, 30°C | Pseudomonas aeruginosa | |
2.3.1.190 | 0.483 | - |
diacetyl | pH 7.4, 30°C | Pseudomonas aeruginosa |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
1.1.1.4 | Zn2+ | zinc binding enzyme | Pseudomonas aeruginosa | |
1.1.1.B20 | Zn2+ | zinc binding enzyme | Pseudomonas aeruginosa | |
1.1.1.76 | Zn2+ | zinc binding enzyme | Pseudomonas aeruginosa |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.1.1.4 | (2R,3S)-butane-2,3-diol + NAD+ | Pseudomonas aeruginosa | - |
(3R)-acetoin + NADH + H+ | - |
? | |
1.1.1.4 | (2R,3S)-butane-2,3-diol + NAD+ | Pseudomonas aeruginosa ATCC 15692 | - |
(3R)-acetoin + NADH + H+ | - |
? | |
1.1.1.4 | (2R,3S)-butane-2,3-diol + NAD+ | Pseudomonas aeruginosa 1C | - |
(3R)-acetoin + NADH + H+ | - |
? | |
1.1.1.4 | (2R,3S)-butane-2,3-diol + NAD+ | Pseudomonas aeruginosa PRS 101 | - |
(3R)-acetoin + NADH + H+ | - |
? | |
1.1.1.4 | (2R,3S)-butane-2,3-diol + NAD+ | Pseudomonas aeruginosa DSM 22644 | - |
(3R)-acetoin + NADH + H+ | - |
? | |
1.1.1.4 | (2R,3S)-butane-2,3-diol + NAD+ | Pseudomonas aeruginosa CIP 104116 | - |
(3R)-acetoin + NADH + H+ | - |
? | |
1.1.1.4 | (2R,3S)-butane-2,3-diol + NAD+ | Pseudomonas aeruginosa LMG 12228 | - |
(3R)-acetoin + NADH + H+ | - |
? | |
1.1.1.4 | (2R,3S)-butane-2,3-diol + NAD+ | Pseudomonas aeruginosa JCM 14847 | - |
(3R)-acetoin + NADH + H+ | - |
? | |
1.1.1.4 | (R,R)-butane-2,3-diol + NAD+ | Pseudomonas aeruginosa | - |
(R)-acetoin + NADH + H+ | - |
r | |
1.1.1.4 | (R,R)-butane-2,3-diol + NAD+ | Pseudomonas aeruginosa ATCC 15692 | - |
(R)-acetoin + NADH + H+ | - |
r | |
1.1.1.4 | (R,R)-butane-2,3-diol + NAD+ | Pseudomonas aeruginosa 1C | - |
(R)-acetoin + NADH + H+ | - |
r | |
1.1.1.4 | (R,R)-butane-2,3-diol + NAD+ | Pseudomonas aeruginosa PRS 101 | - |
(R)-acetoin + NADH + H+ | - |
r | |
1.1.1.4 | (R,R)-butane-2,3-diol + NAD+ | Pseudomonas aeruginosa DSM 22644 | - |
(R)-acetoin + NADH + H+ | - |
r | |
1.1.1.4 | (R,R)-butane-2,3-diol + NAD+ | Pseudomonas aeruginosa CIP 104116 | - |
(R)-acetoin + NADH + H+ | - |
r | |
1.1.1.4 | (R,R)-butane-2,3-diol + NAD+ | Pseudomonas aeruginosa LMG 12228 | - |
(R)-acetoin + NADH + H+ | - |
r | |
1.1.1.4 | (R,R)-butane-2,3-diol + NAD+ | Pseudomonas aeruginosa JCM 14847 | - |
(R)-acetoin + NADH + H+ | - |
r | |
1.1.1.B20 | (2R,3S)-butane-2,3-diol + NAD+ | Pseudomonas aeruginosa | meso-butane-2,3-diol | (3R)-acetoin + NADH + H+ | - |
? | |
1.1.1.B20 | (R,R)-butane-2,3-diol + NAD+ | Pseudomonas aeruginosa | - |
(R)-acetoin + NADH + H+ | - |
r | |
1.1.1.76 | (2S,3S)-butane-2,3-diol + NAD+ | Pseudomonas aeruginosa | - |
(S)-acetoin + NADH + H+ | - |
? | |
1.1.1.76 | (2S,3S)-butane-2,3-diol + NAD+ | Pseudomonas aeruginosa ATCC 15692 | - |
(S)-acetoin + NADH + H+ | - |
? | |
1.1.1.76 | (2S,3S)-butane-2,3-diol + NAD+ | Pseudomonas aeruginosa 1C | - |
(S)-acetoin + NADH + H+ | - |
? | |
1.1.1.76 | (2S,3S)-butane-2,3-diol + NAD+ | Pseudomonas aeruginosa PRS 101 | - |
(S)-acetoin + NADH + H+ | - |
? | |
1.1.1.76 | (2S,3S)-butane-2,3-diol + NAD+ | Pseudomonas aeruginosa DSM 22644 | - |
(S)-acetoin + NADH + H+ | - |
? | |
1.1.1.76 | (2S,3S)-butane-2,3-diol + NAD+ | Pseudomonas aeruginosa CIP 104116 | - |
(S)-acetoin + NADH + H+ | - |
? | |
1.1.1.76 | (2S,3S)-butane-2,3-diol + NAD+ | Pseudomonas aeruginosa LMG 12228 | - |
(S)-acetoin + NADH + H+ | - |
? | |
1.1.1.76 | (2S,3S)-butane-2,3-diol + NAD+ | Pseudomonas aeruginosa JCM 14847 | - |
(S)-acetoin + NADH + H+ | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.1.1.4 | Pseudomonas aeruginosa | Q9HWM8 | - |
- |
1.1.1.4 | Pseudomonas aeruginosa 1C | Q9HWM8 | - |
- |
1.1.1.4 | Pseudomonas aeruginosa ATCC 15692 | Q9HWM8 | - |
- |
1.1.1.4 | Pseudomonas aeruginosa CIP 104116 | Q9HWM8 | - |
- |
1.1.1.4 | Pseudomonas aeruginosa DSM 22644 | Q9HWM8 | - |
- |
1.1.1.4 | Pseudomonas aeruginosa JCM 14847 | Q9HWM8 | - |
- |
1.1.1.4 | Pseudomonas aeruginosa LMG 12228 | Q9HWM8 | - |
- |
1.1.1.4 | Pseudomonas aeruginosa PRS 101 | Q9HWM8 | - |
- |
1.1.1.B20 | Pseudomonas aeruginosa | Q9HWM8 | - |
- |
1.1.1.B20 | Pseudomonas aeruginosa 1C | Q9HWM8 | - |
- |
1.1.1.B20 | Pseudomonas aeruginosa ATCC 15692 | Q9HWM8 | - |
- |
1.1.1.B20 | Pseudomonas aeruginosa CIP 104116 | Q9HWM8 | - |
- |
1.1.1.B20 | Pseudomonas aeruginosa DSM 22644 | Q9HWM8 | - |
- |
1.1.1.B20 | Pseudomonas aeruginosa JCM 14847 | Q9HWM8 | - |
- |
1.1.1.B20 | Pseudomonas aeruginosa LMG 12228 | Q9HWM8 | - |
- |
1.1.1.B20 | Pseudomonas aeruginosa PRS 101 | Q9HWM8 | - |
- |
1.1.1.76 | Pseudomonas aeruginosa | - |
- |
- |
1.1.1.76 | Pseudomonas aeruginosa 1C | - |
- |
- |
1.1.1.76 | Pseudomonas aeruginosa ATCC 15692 | - |
- |
- |
1.1.1.76 | Pseudomonas aeruginosa CIP 104116 | - |
- |
- |
1.1.1.76 | Pseudomonas aeruginosa DSM 22644 | - |
- |
- |
1.1.1.76 | Pseudomonas aeruginosa JCM 14847 | - |
- |
- |
1.1.1.76 | Pseudomonas aeruginosa LMG 12228 | - |
- |
- |
1.1.1.76 | Pseudomonas aeruginosa PRS 101 | - |
- |
- |
2.3.1.190 | Pseudomonas aeruginosa | Q9HWN1 AND Q9HWN0 | Q9HWN1: AcoA, Q9HWN0: AcoB | - |
2.3.1.190 | Pseudomonas aeruginosa ATCC 15692 | Q9HWN1 AND Q9HWN0 | Q9HWN1: AcoA, Q9HWN0: AcoB | - |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.3.1.190 | - |
Pseudomonas aeruginosa |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
1.1.1.4 | 0.155 | - |
pH 7.4, 30°C, substrate propane-1,2-diol | Pseudomonas aeruginosa |
1.1.1.4 | 0.331 | - |
pH 7.4, 30°C, substrate 1-hydroxy-2-butanone | Pseudomonas aeruginosa |
1.1.1.4 | 0.383 | - |
pH 7.4, 30°C, substrate butane-1,2-diol | Pseudomonas aeruginosa |
1.1.1.4 | 1.179 | - |
pH 7.4, 30°C, substrate (2R,3S)-butane-2,3-diol | Pseudomonas aeruginosa |
1.1.1.4 | 1.346 | - |
pH 7.4, 30°C, substrate 2,3-pentanedione | Pseudomonas aeruginosa |
1.1.1.4 | 1.538 | - |
pH 7.4, 30°C, substrate (2R,3R)-butane-2,3-diol | Pseudomonas aeruginosa |
1.1.1.4 | 1.949 | - |
pH 7.4, 30°C, substrate rac acetoin | Pseudomonas aeruginosa |
1.1.1.4 | 2.154 | - |
pH 7.4, 30°C, substrate diacetyl | Pseudomonas aeruginosa |
1.1.1.B20 | 0.155 | - |
pH 7.4, 30°C, substrate propane-1,2-diol | Pseudomonas aeruginosa |
1.1.1.B20 | 0.331 | - |
pH 7.4, 30°C, substrate 1-hydroxy-2-butanone | Pseudomonas aeruginosa |
1.1.1.B20 | 0.383 | - |
pH 7.4, 30°C, substrate butane-1,2-diol | Pseudomonas aeruginosa |
1.1.1.B20 | 1.179 | - |
pH 7.4, 30°C, substrate (2R,3S)-butane-2,3-diol | Pseudomonas aeruginosa |
1.1.1.B20 | 1.346 | - |
pH 7.4, 30°C, substrate 2,3-pentanedione | Pseudomonas aeruginosa |
1.1.1.B20 | 1.538 | - |
pH 7.4, 30°C, substrate (2R,3R)-butane-2,3-diol | Pseudomonas aeruginosa |
1.1.1.B20 | 1.949 | - |
pH 7.4, 30°C, substrate rac acetoin | Pseudomonas aeruginosa |
1.1.1.B20 | 2.154 | - |
pH 7.4, 30°C, substrate diacetyl | Pseudomonas aeruginosa |
1.1.1.76 | 0.194 | - |
pH 7.4, 30°C, substrate butane-1,2-diol | Pseudomonas aeruginosa |
1.1.1.76 | 0.516 | - |
pH 7.4, 30°C, substrate 2-butanol | Pseudomonas aeruginosa |
1.1.1.76 | 0.779 | - |
pH 7.4, 30°C, substrate propane-1,2-diol | Pseudomonas aeruginosa |
1.1.1.76 | 0.84 | - |
pH 7.4, 30°C, substrate (2S,3S)-butane-2,3-diol | Pseudomonas aeruginosa |
1.1.1.76 | 1.671 | - |
pH 7.4, 30°C, substrate n-butanal | Pseudomonas aeruginosa |
1.1.1.76 | 1.837 | - |
pH 7.4, 30°C, substrate 1-hydroxy-2-butanone | Pseudomonas aeruginosa |
1.1.1.76 | 3.238 | - |
pH 7.4, 30°C, substrate rac acetoin | Pseudomonas aeruginosa |
1.1.1.76 | 7.28 | - |
pH 7.4, 30°C, substrate 2,3-pentanedione | Pseudomonas aeruginosa |
1.1.1.76 | 8.286 | - |
pH 7.4, 30°C, substrate diacetyl | Pseudomonas aeruginosa |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.1.1.4 | (2R,3S)-butane-2,3-diol + NAD+ | - |
Pseudomonas aeruginosa | (3R)-acetoin + NADH + H+ | - |
? | |
1.1.1.4 | (2R,3S)-butane-2,3-diol + NAD+ | stereoselective interconversion | Pseudomonas aeruginosa | (3R)-acetoin + NADH + H+ | - |
? | |
1.1.1.4 | (2R,3S)-butane-2,3-diol + NAD+ | - |
Pseudomonas aeruginosa ATCC 15692 | (3R)-acetoin + NADH + H+ | - |
? | |
1.1.1.4 | (2R,3S)-butane-2,3-diol + NAD+ | stereoselective interconversion | Pseudomonas aeruginosa ATCC 15692 | (3R)-acetoin + NADH + H+ | - |
? | |
1.1.1.4 | (2R,3S)-butane-2,3-diol + NAD+ | - |
Pseudomonas aeruginosa 1C | (3R)-acetoin + NADH + H+ | - |
? | |
1.1.1.4 | (2R,3S)-butane-2,3-diol + NAD+ | stereoselective interconversion | Pseudomonas aeruginosa 1C | (3R)-acetoin + NADH + H+ | - |
? | |
1.1.1.4 | (2R,3S)-butane-2,3-diol + NAD+ | - |
Pseudomonas aeruginosa PRS 101 | (3R)-acetoin + NADH + H+ | - |
? | |
1.1.1.4 | (2R,3S)-butane-2,3-diol + NAD+ | stereoselective interconversion | Pseudomonas aeruginosa PRS 101 | (3R)-acetoin + NADH + H+ | - |
? | |
1.1.1.4 | (2R,3S)-butane-2,3-diol + NAD+ | - |
Pseudomonas aeruginosa DSM 22644 | (3R)-acetoin + NADH + H+ | - |
? | |
1.1.1.4 | (2R,3S)-butane-2,3-diol + NAD+ | stereoselective interconversion | Pseudomonas aeruginosa DSM 22644 | (3R)-acetoin + NADH + H+ | - |
? | |
1.1.1.4 | (2R,3S)-butane-2,3-diol + NAD+ | - |
Pseudomonas aeruginosa CIP 104116 | (3R)-acetoin + NADH + H+ | - |
? | |
1.1.1.4 | (2R,3S)-butane-2,3-diol + NAD+ | stereoselective interconversion | Pseudomonas aeruginosa CIP 104116 | (3R)-acetoin + NADH + H+ | - |
? | |
1.1.1.4 | (2R,3S)-butane-2,3-diol + NAD+ | - |
Pseudomonas aeruginosa LMG 12228 | (3R)-acetoin + NADH + H+ | - |
? | |
1.1.1.4 | (2R,3S)-butane-2,3-diol + NAD+ | stereoselective interconversion | Pseudomonas aeruginosa LMG 12228 | (3R)-acetoin + NADH + H+ | - |
? | |
1.1.1.4 | (2R,3S)-butane-2,3-diol + NAD+ | - |
Pseudomonas aeruginosa JCM 14847 | (3R)-acetoin + NADH + H+ | - |
? | |
1.1.1.4 | (2R,3S)-butane-2,3-diol + NAD+ | stereoselective interconversion | Pseudomonas aeruginosa JCM 14847 | (3R)-acetoin + NADH + H+ | - |
? | |
1.1.1.4 | (R,R)-butane-2,3-diol + NAD+ | - |
Pseudomonas aeruginosa | (R)-acetoin + NADH + H+ | - |
r | |
1.1.1.4 | (R,R)-butane-2,3-diol + NAD+ | - |
Pseudomonas aeruginosa ATCC 15692 | (R)-acetoin + NADH + H+ | - |
r | |
1.1.1.4 | (R,R)-butane-2,3-diol + NAD+ | - |
Pseudomonas aeruginosa 1C | (R)-acetoin + NADH + H+ | - |
r | |
1.1.1.4 | (R,R)-butane-2,3-diol + NAD+ | - |
Pseudomonas aeruginosa PRS 101 | (R)-acetoin + NADH + H+ | - |
r | |
1.1.1.4 | (R,R)-butane-2,3-diol + NAD+ | - |
Pseudomonas aeruginosa DSM 22644 | (R)-acetoin + NADH + H+ | - |
r | |
1.1.1.4 | (R,R)-butane-2,3-diol + NAD+ | - |
Pseudomonas aeruginosa CIP 104116 | (R)-acetoin + NADH + H+ | - |
r | |
1.1.1.4 | (R,R)-butane-2,3-diol + NAD+ | - |
Pseudomonas aeruginosa LMG 12228 | (R)-acetoin + NADH + H+ | - |
r | |
1.1.1.4 | (R,R)-butane-2,3-diol + NAD+ | - |
Pseudomonas aeruginosa JCM 14847 | (R)-acetoin + NADH + H+ | - |
r | |
1.1.1.4 | (R,R)-butane-2,3-diol + NAD+ | stereoselective interconversion | Pseudomonas aeruginosa | (3R)-acetoin + NADH + H+ | - |
r | |
1.1.1.4 | (R,R)-butane-2,3-diol + NAD+ | stereoselective interconversion | Pseudomonas aeruginosa ATCC 15692 | (3R)-acetoin + NADH + H+ | - |
r | |
1.1.1.4 | (R,R)-butane-2,3-diol + NAD+ | stereoselective interconversion | Pseudomonas aeruginosa 1C | (3R)-acetoin + NADH + H+ | - |
r | |
1.1.1.4 | (R,R)-butane-2,3-diol + NAD+ | stereoselective interconversion | Pseudomonas aeruginosa PRS 101 | (3R)-acetoin + NADH + H+ | - |
r | |
1.1.1.4 | (R,R)-butane-2,3-diol + NAD+ | stereoselective interconversion | Pseudomonas aeruginosa DSM 22644 | (3R)-acetoin + NADH + H+ | - |
r | |
1.1.1.4 | (R,R)-butane-2,3-diol + NAD+ | stereoselective interconversion | Pseudomonas aeruginosa CIP 104116 | (3R)-acetoin + NADH + H+ | - |
r | |
1.1.1.4 | (R,R)-butane-2,3-diol + NAD+ | stereoselective interconversion | Pseudomonas aeruginosa LMG 12228 | (3R)-acetoin + NADH + H+ | - |
r | |
1.1.1.4 | (R,R)-butane-2,3-diol + NAD+ | stereoselective interconversion | Pseudomonas aeruginosa JCM 14847 | (3R)-acetoin + NADH + H+ | - |
r | |
1.1.1.4 | 1-hydroxy-2-butanone + NADH + H+ | - |
Pseudomonas aeruginosa | ? + NAD+ | - |
? | |
1.1.1.4 | 2,3-pentandione + NADH + H+ | - |
Pseudomonas aeruginosa | ? + NAD+ | - |
? | |
1.1.1.4 | butane-1,2-diol + NAD+ | - |
Pseudomonas aeruginosa | ? + NADH + H+ | - |
? | |
1.1.1.4 | diacetyl + 2 NADH + 2 H+ | - |
Pseudomonas aeruginosa | (2R,3R)-butane-2,3-diol + 2 NAD+ | - |
r | |
1.1.1.4 | diacetyl + NADH + H+ | - |
Pseudomonas aeruginosa | (3R)-acetoin + NAD+ | - |
r | |
1.1.1.4 | additional information | no activity with (2S,3S)-2,3-butane-2,3-diol, but the (2R,3R)-2,3-butanediol dehydrogenase is also active with meso-2,3-butanediol. No activity with 2-butanol, 1,3-butanediol, 1,2-pentanediol, 1,3-propanediol, and glycerol in the oxidation reaction. And no activity with 2,4-pentanedione, butanone, 2,5-hexanedione, n-butanal and 1,3-dihydroxypropanone in the reduction reaction. Substrate specificity, overview. (2R,3R)-2,3-BDH reduces diacetyl into (3R)-acetoin and (2R,3R)-2,3-BD, while racemic acetoin is reduced into (2R,3R)-2,3-BD and meso-2,3-BD | Pseudomonas aeruginosa | ? | - |
- |
|
1.1.1.4 | additional information | no activity with (2S,3S)-2,3-butane-2,3-diol, but the (2R,3R)-2,3-butanediol dehydrogenase is also active with meso-2,3-butanediol. No activity with 2-butanol, 1,3-butanediol, 1,2-pentanediol, 1,3-propanediol, and glycerol in the oxidation reaction. And no activity with 2,4-pentanedione, butanone, 2,5-hexanedione, n-butanal and 1,3-dihydroxypropanone in the reduction reaction. Substrate specificity, overview. (2R,3R)-2,3-BDH reduces diacetyl into (3R)-acetoin and (2R,3R)-2,3-BD, while racemic acetoin is reduced into (2R,3R)-2,3-BD and meso-2,3-BD | Pseudomonas aeruginosa ATCC 15692 | ? | - |
- |
|
1.1.1.4 | additional information | no activity with (2S,3S)-2,3-butane-2,3-diol, but the (2R,3R)-2,3-butanediol dehydrogenase is also active with meso-2,3-butanediol. No activity with 2-butanol, 1,3-butanediol, 1,2-pentanediol, 1,3-propanediol, and glycerol in the oxidation reaction. And no activity with 2,4-pentanedione, butanone, 2,5-hexanedione, n-butanal and 1,3-dihydroxypropanone in the reduction reaction. Substrate specificity, overview. (2R,3R)-2,3-BDH reduces diacetyl into (3R)-acetoin and (2R,3R)-2,3-BD, while racemic acetoin is reduced into (2R,3R)-2,3-BD and meso-2,3-BD | Pseudomonas aeruginosa 1C | ? | - |
- |
|
1.1.1.4 | additional information | no activity with (2S,3S)-2,3-butane-2,3-diol, but the (2R,3R)-2,3-butanediol dehydrogenase is also active with meso-2,3-butanediol. No activity with 2-butanol, 1,3-butanediol, 1,2-pentanediol, 1,3-propanediol, and glycerol in the oxidation reaction. And no activity with 2,4-pentanedione, butanone, 2,5-hexanedione, n-butanal and 1,3-dihydroxypropanone in the reduction reaction. Substrate specificity, overview. (2R,3R)-2,3-BDH reduces diacetyl into (3R)-acetoin and (2R,3R)-2,3-BD, while racemic acetoin is reduced into (2R,3R)-2,3-BD and meso-2,3-BD | Pseudomonas aeruginosa PRS 101 | ? | - |
- |
|
1.1.1.4 | additional information | no activity with (2S,3S)-2,3-butane-2,3-diol, but the (2R,3R)-2,3-butanediol dehydrogenase is also active with meso-2,3-butanediol. No activity with 2-butanol, 1,3-butanediol, 1,2-pentanediol, 1,3-propanediol, and glycerol in the oxidation reaction. And no activity with 2,4-pentanedione, butanone, 2,5-hexanedione, n-butanal and 1,3-dihydroxypropanone in the reduction reaction. Substrate specificity, overview. (2R,3R)-2,3-BDH reduces diacetyl into (3R)-acetoin and (2R,3R)-2,3-BD, while racemic acetoin is reduced into (2R,3R)-2,3-BD and meso-2,3-BD | Pseudomonas aeruginosa DSM 22644 | ? | - |
- |
|
1.1.1.4 | additional information | no activity with (2S,3S)-2,3-butane-2,3-diol, but the (2R,3R)-2,3-butanediol dehydrogenase is also active with meso-2,3-butanediol. No activity with 2-butanol, 1,3-butanediol, 1,2-pentanediol, 1,3-propanediol, and glycerol in the oxidation reaction. And no activity with 2,4-pentanedione, butanone, 2,5-hexanedione, n-butanal and 1,3-dihydroxypropanone in the reduction reaction. Substrate specificity, overview. (2R,3R)-2,3-BDH reduces diacetyl into (3R)-acetoin and (2R,3R)-2,3-BD, while racemic acetoin is reduced into (2R,3R)-2,3-BD and meso-2,3-BD | Pseudomonas aeruginosa CIP 104116 | ? | - |
- |
|
1.1.1.4 | additional information | no activity with (2S,3S)-2,3-butane-2,3-diol, but the (2R,3R)-2,3-butanediol dehydrogenase is also active with meso-2,3-butanediol. No activity with 2-butanol, 1,3-butanediol, 1,2-pentanediol, 1,3-propanediol, and glycerol in the oxidation reaction. And no activity with 2,4-pentanedione, butanone, 2,5-hexanedione, n-butanal and 1,3-dihydroxypropanone in the reduction reaction. Substrate specificity, overview. (2R,3R)-2,3-BDH reduces diacetyl into (3R)-acetoin and (2R,3R)-2,3-BD, while racemic acetoin is reduced into (2R,3R)-2,3-BD and meso-2,3-BD | Pseudomonas aeruginosa LMG 12228 | ? | - |
- |
|
1.1.1.4 | additional information | no activity with (2S,3S)-2,3-butane-2,3-diol, but the (2R,3R)-2,3-butanediol dehydrogenase is also active with meso-2,3-butanediol. No activity with 2-butanol, 1,3-butanediol, 1,2-pentanediol, 1,3-propanediol, and glycerol in the oxidation reaction. And no activity with 2,4-pentanedione, butanone, 2,5-hexanedione, n-butanal and 1,3-dihydroxypropanone in the reduction reaction. Substrate specificity, overview. (2R,3R)-2,3-BDH reduces diacetyl into (3R)-acetoin and (2R,3R)-2,3-BD, while racemic acetoin is reduced into (2R,3R)-2,3-BD and meso-2,3-BD | Pseudomonas aeruginosa JCM 14847 | ? | - |
- |
|
1.1.1.4 | propane-1,2-diol + NAD+ | - |
Pseudomonas aeruginosa | ? + NADH + H+ | - |
? | |
1.1.1.4 | rac acetoin + NADH + H+ | - |
Pseudomonas aeruginosa | (2R,3R)-butane-2,3-diol + NAD+ | - |
? | |
1.1.1.4 | rac acetoin + NADH + H+ | - |
Pseudomonas aeruginosa | (2R,3S)-butane-2,3-diol + NAD+ | - |
? | |
1.1.1.B20 | (2R,3S)-butane-2,3-diol + NAD+ | meso-butane-2,3-diol | Pseudomonas aeruginosa | (3R)-acetoin + NADH + H+ | - |
? | |
1.1.1.B20 | (2R,3S)-butane-2,3-diol + NAD+ | stereoselective interconversion | Pseudomonas aeruginosa | (3R)-acetoin + NADH + H+ | - |
? | |
1.1.1.B20 | (R,R)-butane-2,3-diol + NAD+ | - |
Pseudomonas aeruginosa | (R)-acetoin + NADH + H+ | - |
r | |
1.1.1.B20 | (R,R)-butane-2,3-diol + NAD+ | stereoselective interconversion | Pseudomonas aeruginosa | (3R)-acetoin + NADH + H+ | - |
r | |
1.1.1.B20 | 1-hydroxy-2-butanone + NADH + H+ | - |
Pseudomonas aeruginosa | ? + NAD+ | - |
? | |
1.1.1.B20 | 2,3-pentandione + NADH + H+ | - |
Pseudomonas aeruginosa | ? + NAD+ | - |
? | |
1.1.1.B20 | butane-1,2-diol + NAD+ | - |
Pseudomonas aeruginosa | ? + NADH + H+ | - |
? | |
1.1.1.B20 | diacetyl + 2 NADH + 2 H+ | - |
Pseudomonas aeruginosa | (2R,3R)-butane-2,3-diol + 2 NAD+ | - |
r | |
1.1.1.B20 | diacetyl + NADH + H+ | - |
Pseudomonas aeruginosa | (3R)-acetoin + NAD+ | - |
r | |
1.1.1.B20 | additional information | the (2R,3R)-2,3-butanediol dehydrogenase is active with (R,R)-butane-2,3-diol, but also with meso-butane-2,3-diol, but not with with (2S,3S)-2,3-butane-2,3-diol. No activity with 2-butanol, 1,3-butanediol, 1,2-pentanediol, 1,3-propanediol, and glycerol in the oxidation reaction. And no activity with 2,4-pentanedione, butanone, 2,5-hexanedione, n-butanal and 1,3-dihydroxypropanone in the reduction reaction. Substrate specificity, overview. (2R,3R)-2,3-BDH reduces diacetyl into (3R)-acetoin and (2R,3R)-2,3-BD, while racemic acetoin is reduced into (2R,3R)-2,3-BD and meso-2,3-BD | Pseudomonas aeruginosa | ? | - |
- |
|
1.1.1.B20 | propane-1,2-diol + NAD+ | - |
Pseudomonas aeruginosa | ? + NADH + H+ | - |
? | |
1.1.1.B20 | rac acetoin + NADH + H+ | - |
Pseudomonas aeruginosa | (2R,3R)-butane-2,3-diol + NAD+ | - |
? | |
1.1.1.B20 | rac acetoin + NADH + H+ | - |
Pseudomonas aeruginosa | (2R,3S)-butane-2,3-diol + NAD+ | - |
? | |
1.1.1.76 | (2S,3S)-butane-2,3-diol + NAD+ | - |
Pseudomonas aeruginosa | (S)-acetoin + NADH + H+ | - |
? | |
1.1.1.76 | (2S,3S)-butane-2,3-diol + NAD+ | stereoselective interconversion | Pseudomonas aeruginosa | (S)-acetoin + NADH + H+ | - |
? | |
1.1.1.76 | (2S,3S)-butane-2,3-diol + NAD+ | - |
Pseudomonas aeruginosa ATCC 15692 | (S)-acetoin + NADH + H+ | - |
? | |
1.1.1.76 | (2S,3S)-butane-2,3-diol + NAD+ | stereoselective interconversion | Pseudomonas aeruginosa ATCC 15692 | (S)-acetoin + NADH + H+ | - |
? | |
1.1.1.76 | (2S,3S)-butane-2,3-diol + NAD+ | - |
Pseudomonas aeruginosa 1C | (S)-acetoin + NADH + H+ | - |
? | |
1.1.1.76 | (2S,3S)-butane-2,3-diol + NAD+ | stereoselective interconversion | Pseudomonas aeruginosa 1C | (S)-acetoin + NADH + H+ | - |
? | |
1.1.1.76 | (2S,3S)-butane-2,3-diol + NAD+ | - |
Pseudomonas aeruginosa PRS 101 | (S)-acetoin + NADH + H+ | - |
? | |
1.1.1.76 | (2S,3S)-butane-2,3-diol + NAD+ | stereoselective interconversion | Pseudomonas aeruginosa PRS 101 | (S)-acetoin + NADH + H+ | - |
? | |
1.1.1.76 | (2S,3S)-butane-2,3-diol + NAD+ | - |
Pseudomonas aeruginosa DSM 22644 | (S)-acetoin + NADH + H+ | - |
? | |
1.1.1.76 | (2S,3S)-butane-2,3-diol + NAD+ | stereoselective interconversion | Pseudomonas aeruginosa DSM 22644 | (S)-acetoin + NADH + H+ | - |
? | |
1.1.1.76 | (2S,3S)-butane-2,3-diol + NAD+ | - |
Pseudomonas aeruginosa CIP 104116 | (S)-acetoin + NADH + H+ | - |
? | |
1.1.1.76 | (2S,3S)-butane-2,3-diol + NAD+ | stereoselective interconversion | Pseudomonas aeruginosa CIP 104116 | (S)-acetoin + NADH + H+ | - |
? | |
1.1.1.76 | (2S,3S)-butane-2,3-diol + NAD+ | - |
Pseudomonas aeruginosa LMG 12228 | (S)-acetoin + NADH + H+ | - |
? | |
1.1.1.76 | (2S,3S)-butane-2,3-diol + NAD+ | stereoselective interconversion | Pseudomonas aeruginosa LMG 12228 | (S)-acetoin + NADH + H+ | - |
? | |
1.1.1.76 | (2S,3S)-butane-2,3-diol + NAD+ | - |
Pseudomonas aeruginosa JCM 14847 | (S)-acetoin + NADH + H+ | - |
? | |
1.1.1.76 | (2S,3S)-butane-2,3-diol + NAD+ | stereoselective interconversion | Pseudomonas aeruginosa JCM 14847 | (S)-acetoin + NADH + H+ | - |
? | |
1.1.1.76 | 1-hydroxy-2-butanone + NADH + H+ | - |
Pseudomonas aeruginosa | ? + NAD+ | - |
? | |
1.1.1.76 | 1-hydroxy-2-butanone + NADH + H+ | - |
Pseudomonas aeruginosa ATCC 15692 | ? + NAD+ | - |
? | |
1.1.1.76 | 1-hydroxy-2-butanone + NADH + H+ | - |
Pseudomonas aeruginosa 1C | ? + NAD+ | - |
? | |
1.1.1.76 | 1-hydroxy-2-butanone + NADH + H+ | - |
Pseudomonas aeruginosa PRS 101 | ? + NAD+ | - |
? | |
1.1.1.76 | 1-hydroxy-2-butanone + NADH + H+ | - |
Pseudomonas aeruginosa DSM 22644 | ? + NAD+ | - |
? | |
1.1.1.76 | 1-hydroxy-2-butanone + NADH + H+ | - |
Pseudomonas aeruginosa CIP 104116 | ? + NAD+ | - |
? | |
1.1.1.76 | 1-hydroxy-2-butanone + NADH + H+ | - |
Pseudomonas aeruginosa LMG 12228 | ? + NAD+ | - |
? | |
1.1.1.76 | 1-hydroxy-2-butanone + NADH + H+ | - |
Pseudomonas aeruginosa JCM 14847 | ? + NAD+ | - |
? | |
1.1.1.76 | 2 diacetyl + 2 NADH + 2 H+ | - |
Pseudomonas aeruginosa | (3S)-acetoin + (2S,3S)-butane-2,3-diol + NAD+ | - |
? | |
1.1.1.76 | 2 rac acetoin + 2 NADH + 2 H+ | - |
Pseudomonas aeruginosa | (2S,3S)-butane-2,3-diol + (2R,3S)-butane-2,3-diol + 2 NAD+ | - |
? | |
1.1.1.76 | 2,3-pentandione + NADH + H+ | - |
Pseudomonas aeruginosa | ? + NAD+ | - |
? | |
1.1.1.76 | 2-butanol + NAD+ | - |
Pseudomonas aeruginosa | 2-butanone + NADH + H+ | - |
ir | |
1.1.1.76 | 2-butanol + NAD+ | - |
Pseudomonas aeruginosa ATCC 15692 | 2-butanone + NADH + H+ | - |
ir | |
1.1.1.76 | 2-butanol + NAD+ | - |
Pseudomonas aeruginosa 1C | 2-butanone + NADH + H+ | - |
ir | |
1.1.1.76 | 2-butanol + NAD+ | - |
Pseudomonas aeruginosa PRS 101 | 2-butanone + NADH + H+ | - |
ir | |
1.1.1.76 | 2-butanol + NAD+ | - |
Pseudomonas aeruginosa DSM 22644 | 2-butanone + NADH + H+ | - |
ir | |
1.1.1.76 | 2-butanol + NAD+ | - |
Pseudomonas aeruginosa CIP 104116 | 2-butanone + NADH + H+ | - |
ir | |
1.1.1.76 | 2-butanol + NAD+ | - |
Pseudomonas aeruginosa LMG 12228 | 2-butanone + NADH + H+ | - |
ir | |
1.1.1.76 | 2-butanol + NAD+ | - |
Pseudomonas aeruginosa JCM 14847 | 2-butanone + NADH + H+ | - |
ir | |
1.1.1.76 | butane-1,2-diol + NAD+ | - |
Pseudomonas aeruginosa | ? + NADH + H+ | - |
? | |
1.1.1.76 | additional information | no activity with (2R,3R)-butane-2,3-diol and (2R,3S)-butane-2,3-diol, and no activity with 1,3-butanediol, 1,2-pentanediol, 1,3-propanediol, and glycerol in the oxidation reaction. No activity with 2,4-pentanedione, butanone, 2,5-hexanedione, and 1,3-dihydroxypropanone in the reduction reaction. Substrate specificity, overview. (2S,3S)-2,3-BDH reduces diacetyl into (3S)-acetoin and (2S,3S)-2,3-BD, while racemic acetoin is reduced to form (2S,3S)-2,3-BD and meso-2,3-BD | Pseudomonas aeruginosa | ? | - |
- |
|
1.1.1.76 | additional information | no activity with (2R,3R)-butane-2,3-diol and (2R,3S)-butane-2,3-diol, and no activity with 1,3-butanediol, 1,2-pentanediol, 1,3-propanediol, and glycerol in the oxidation reaction. No activity with 2,4-pentanedione, butanone, 2,5-hexanedione, and 1,3-dihydroxypropanone in the reduction reaction. Substrate specificity, overview. (2S,3S)-2,3-BDH reduces diacetyl into (3S)-acetoin and (2S,3S)-2,3-BD, while racemic acetoin is reduced to form (2S,3S)-2,3-BD and meso-2,3-BD | Pseudomonas aeruginosa ATCC 15692 | ? | - |
- |
|
1.1.1.76 | additional information | no activity with (2R,3R)-butane-2,3-diol and (2R,3S)-butane-2,3-diol, and no activity with 1,3-butanediol, 1,2-pentanediol, 1,3-propanediol, and glycerol in the oxidation reaction. No activity with 2,4-pentanedione, butanone, 2,5-hexanedione, and 1,3-dihydroxypropanone in the reduction reaction. Substrate specificity, overview. (2S,3S)-2,3-BDH reduces diacetyl into (3S)-acetoin and (2S,3S)-2,3-BD, while racemic acetoin is reduced to form (2S,3S)-2,3-BD and meso-2,3-BD | Pseudomonas aeruginosa 1C | ? | - |
- |
|
1.1.1.76 | additional information | no activity with (2R,3R)-butane-2,3-diol and (2R,3S)-butane-2,3-diol, and no activity with 1,3-butanediol, 1,2-pentanediol, 1,3-propanediol, and glycerol in the oxidation reaction. No activity with 2,4-pentanedione, butanone, 2,5-hexanedione, and 1,3-dihydroxypropanone in the reduction reaction. Substrate specificity, overview. (2S,3S)-2,3-BDH reduces diacetyl into (3S)-acetoin and (2S,3S)-2,3-BD, while racemic acetoin is reduced to form (2S,3S)-2,3-BD and meso-2,3-BD | Pseudomonas aeruginosa PRS 101 | ? | - |
- |
|
1.1.1.76 | additional information | no activity with (2R,3R)-butane-2,3-diol and (2R,3S)-butane-2,3-diol, and no activity with 1,3-butanediol, 1,2-pentanediol, 1,3-propanediol, and glycerol in the oxidation reaction. No activity with 2,4-pentanedione, butanone, 2,5-hexanedione, and 1,3-dihydroxypropanone in the reduction reaction. Substrate specificity, overview. (2S,3S)-2,3-BDH reduces diacetyl into (3S)-acetoin and (2S,3S)-2,3-BD, while racemic acetoin is reduced to form (2S,3S)-2,3-BD and meso-2,3-BD | Pseudomonas aeruginosa DSM 22644 | ? | - |
- |
|
1.1.1.76 | additional information | no activity with (2R,3R)-butane-2,3-diol and (2R,3S)-butane-2,3-diol, and no activity with 1,3-butanediol, 1,2-pentanediol, 1,3-propanediol, and glycerol in the oxidation reaction. No activity with 2,4-pentanedione, butanone, 2,5-hexanedione, and 1,3-dihydroxypropanone in the reduction reaction. Substrate specificity, overview. (2S,3S)-2,3-BDH reduces diacetyl into (3S)-acetoin and (2S,3S)-2,3-BD, while racemic acetoin is reduced to form (2S,3S)-2,3-BD and meso-2,3-BD | Pseudomonas aeruginosa CIP 104116 | ? | - |
- |
|
1.1.1.76 | additional information | no activity with (2R,3R)-butane-2,3-diol and (2R,3S)-butane-2,3-diol, and no activity with 1,3-butanediol, 1,2-pentanediol, 1,3-propanediol, and glycerol in the oxidation reaction. No activity with 2,4-pentanedione, butanone, 2,5-hexanedione, and 1,3-dihydroxypropanone in the reduction reaction. Substrate specificity, overview. (2S,3S)-2,3-BDH reduces diacetyl into (3S)-acetoin and (2S,3S)-2,3-BD, while racemic acetoin is reduced to form (2S,3S)-2,3-BD and meso-2,3-BD | Pseudomonas aeruginosa LMG 12228 | ? | - |
- |
|
1.1.1.76 | additional information | no activity with (2R,3R)-butane-2,3-diol and (2R,3S)-butane-2,3-diol, and no activity with 1,3-butanediol, 1,2-pentanediol, 1,3-propanediol, and glycerol in the oxidation reaction. No activity with 2,4-pentanedione, butanone, 2,5-hexanedione, and 1,3-dihydroxypropanone in the reduction reaction. Substrate specificity, overview. (2S,3S)-2,3-BDH reduces diacetyl into (3S)-acetoin and (2S,3S)-2,3-BD, while racemic acetoin is reduced to form (2S,3S)-2,3-BD and meso-2,3-BD | Pseudomonas aeruginosa JCM 14847 | ? | - |
- |
|
1.1.1.76 | n-butanal + NADH + H+ | - |
Pseudomonas aeruginosa | ? + NAD+ | - |
? | |
1.1.1.76 | propane-1,2-diol + NAD+ | - |
Pseudomonas aeruginosa | ? + NADH + H+ | - |
? | |
2.3.1.190 | acetoin + CoA + NAD+ | - |
Pseudomonas aeruginosa | acetaldehyde + acetyl-CoA + NADH + H+ | - |
? | |
2.3.1.190 | acetoin + CoA + NAD+ | - |
Pseudomonas aeruginosa ATCC 15692 | acetaldehyde + acetyl-CoA + NADH + H+ | - |
? | |
2.3.1.190 | diacetyl + CoA + NAD+ | - |
Pseudomonas aeruginosa | ? | - |
? | |
2.3.1.190 | diacetyl + CoA + NAD+ | - |
Pseudomonas aeruginosa ATCC 15692 | ? | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.1.1.4 | (2R,3R)-2,3-BDH | - |
Pseudomonas aeruginosa |
1.1.1.4 | (2R,3R)-2,3-butanediol dehydrogenase | - |
Pseudomonas aeruginosa |
1.1.1.4 | BDH | - |
Pseudomonas aeruginosa |
1.1.1.4 | More | cf. EC 1.1.1.303 | Pseudomonas aeruginosa |
1.1.1.4 | PA4153 | locus name | Pseudomonas aeruginosa |
1.1.1.B20 | BDH | - |
Pseudomonas aeruginosa |
1.1.1.B20 | More | cf. EC 1.1.1.303 | Pseudomonas aeruginosa |
1.1.1.B20 | PA4153 | locus name | Pseudomonas aeruginosa |
1.1.1.76 | (2R,3R)-2,3-BDH | - |
Pseudomonas aeruginosa |
1.1.1.76 | (2R,3R)-2,3-butanediol dehydrogenase | - |
Pseudomonas aeruginosa |
1.1.1.76 | BDH | - |
Pseudomonas aeruginosa |
1.1.1.76 | More | cf. EC 1.1.1.304 | Pseudomonas aeruginosa |
2.3.1.190 | acetoin dehydrogenase enzyme system | consists of TPP-dependent acetoin dehydrogenase (AoDH E1), dihydrolipoamide acetyltransferase (AoDH E2) and dihydrolipoamide dehydrogenase (AoDH E3) | Pseudomonas aeruginosa |
2.3.1.190 | AcoAB | - |
Pseudomonas aeruginosa |
2.3.1.190 | PA4150 | - |
Pseudomonas aeruginosa |
2.3.1.190 | PA4151 | - |
Pseudomonas aeruginosa |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.1.1.4 | 30 | - |
assay at | Pseudomonas aeruginosa |
1.1.1.B20 | 30 | - |
assay at | Pseudomonas aeruginosa |
1.1.1.76 | 30 | - |
assay at | Pseudomonas aeruginosa |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.1.1.4 | 7.4 | - |
assay at | Pseudomonas aeruginosa |
1.1.1.B20 | 7.4 | - |
assay at | Pseudomonas aeruginosa |
1.1.1.76 | 7.4 | - |
assay at | Pseudomonas aeruginosa |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.1.1.4 | NAD+ | - |
Pseudomonas aeruginosa | |
1.1.1.4 | NADH | - |
Pseudomonas aeruginosa | |
1.1.1.B20 | NAD+ | - |
Pseudomonas aeruginosa | |
1.1.1.B20 | NADH | - |
Pseudomonas aeruginosa | |
1.1.1.76 | NAD+ | - |
Pseudomonas aeruginosa | |
1.1.1.76 | NADH | - |
Pseudomonas aeruginosa | |
2.3.1.190 | thiamine diphosphate | enzyme is dependent on | Pseudomonas aeruginosa |
EC Number | General Information | Comment | Organism |
---|---|---|---|
1.1.1.4 | malfunction | the amount of (2R,3R)-2,3-BD is highly reduced in a DELTAacoR mutant lacking the regulatory protein AcoR. The loss of locus pa4153, encoding (2R,3R)-2,3-BDH, has no effect on the ability of this strain to grow in (2S,3S)-2,3-BD but completely impairs its ability to utilize (2R,3R)-2,3-BD and meso-2,3-BD. The complementation of the pa4153 mutant strain with its gene successfully restores the growth ability. The DELTApa4153 PAO1 strain can grow in racemic acetoin, indicating that (2R,3R)-2,3-BDH contributes to 2,3-BD utilization by converting 2,3-BD into acetoin | Pseudomonas aeruginosa |
1.1.1.4 | physiological function | 22,3-butanediol (2,3-BD) exists in three stereoisomeric forms: (2R,3R)-2,3-BD, meso-2,3-BD and (2S,3S)-2,3-BD. All three stereoisomers are transformed into acetoin by (2R,3R)-2,3-butanediol dehydrogenase (BDH) or (2S,3S)-2,3-BDH. Acetoin is cleaved to form acetyl-CoA and acetaldehyde by acetoin dehydrogenase enzyme system (AoDH ES). Genes encoding (2R,3R)-2,3-BDH, (2S,3S)-2,3-BDH and the E1 and E2 components of AoDH ES are identified as part of a 2,3-BD utilization operon. In addition, the regulatory protein AcoR promotes the expression of this operon using acetaldehyde, a cleavage product of acetoin, as its direct effector. Proposed model for 2,3-BD utilization in Pseudomonas aeruginosa strain PAO1 in downstream catabolic pathways, overview. Genes pa4148, pa4149, pa4150, pa4151, pa4152 and pa4153 comprise an operon responsible for 2,3-BD utilization, mutational analysis. Acetaldehyde is the direct inducer of the 2,3-BD utilization operon | Pseudomonas aeruginosa |
1.1.1.B20 | malfunction | the amount of meso-2,3-BD is highly reduced in a DELTAacoR mutant lacking the regulatory protein AcoR. The loss of locus pa4153, encoding (2R,3R)-2,3-BDH, has no effect on the ability of this strain to grow in (2S,3S)-2,3-BD but completely impairs its ability to utilize (2R,3R)-2,3-BD and meso-2,3-BD. The complementation of the pa4153 mutant strain with its gene successfully restores the growth ability. The DELTApa4153 PAO1 strain can grow in racemic acetoin, indicating that (2R,3R)-2,3-BDH contributes to 2,3-BD utilization by converting 2,3-BD into acetoin | Pseudomonas aeruginosa |
1.1.1.B20 | metabolism | 2,3-butanediol (2,3-BD) exists in three stereoisomeric forms: (2R,3R)-2,3-BD, meso-2,3-BD and (2S,3S)-2,3-BD. All three stereoisomers are transformed into acetoin by (2R,3R)-2,3-butanediol dehydrogenase (BDH) or (2S,3S)-2,3-BDH. Acetoin is cleaved to form acetyl-CoA and acetaldehyde by acetoin dehydrogenase enzyme system (AoDH ES). Genes encoding (2R,3R)-2,3-BDH, (2S,3S)-2,3-BDH and the E1 and E2 components of AoDH ES are identified as part of a 2,3-BD utilization operon. In addition, the regulatory protein AcoR promotes the expression of this operon using acetaldehyde, a cleavage product of acetoin, as its direct effector. Proposed model for 2,3-BD utilization in Pseudomonas aeruginosa strain PAO1 in downstream catabolic pathways, overview. Genes pa4148, pa4149, pa4150, pa4151, pa4152 and pa4153 comprise an operon responsible for 2,3-BD utilization, mutational analysis. Acetaldehyde is the direct inducer of the 2,3-BD utilization operon | Pseudomonas aeruginosa |
1.1.1.B20 | physiological function | 2,3-butanediol (2,3-BD) is a primary microbial metabolite that enhances the virulence of Pseudomonas aeruginosa and alters the lung microbiome. 2,3-BD exists in three stereoisomeric forms: (2R,3R)-2,3-BD, meso-2,3-BD and (2S,3S)-2,3-BD | Pseudomonas aeruginosa |
1.1.1.76 | malfunction | the amount of (2S,3S)-2,3-BD is highly reduced in a DELTAacoR mutant lacking the regulatory protein AcoR. The disruption of locus pa4148, encoding (2S,3S)-2,3-BDH, partially impairs the growth of this strain in (2S,3S)-2,3-BD but does not affect its growth in (2R,3R)-2,3-BD and meso-2,3-BD. The complementation of the pa4148 mutant strain with its gene successfully restores the growth ability. The DELTApa4148 PAO1 strain can grow in racemic acetoin, indicating that (2S,3S)-2,3-BDH contributes to 2,3-BD utilization by converting 2,3-BD into acetoin | Pseudomonas aeruginosa |
1.1.1.76 | metabolism | 2,3-butanediol (2,3-BD) exists in three stereoisomeric forms: (2R,3R)-2,3-BD, meso-2,3-BD and (2S,3S)-2,3-BD. All three stereoisomers are transformed into acetoin by (2R,3R)-2,3-butanediol dehydrogenase (BDH) or (2S,3S)-2,3-BDH. Acetoin is cleaved to form acetyl-CoA and acetaldehyde by acetoin dehydrogenase enzyme system (AoDH ES). Genes encoding (2R,3R)-2,3-BDH, (2S,3S)-2,3-BDH and the E1 and E2 components of AoDH ES are identified as part of a 2,3-BD utilization operon. In addition, the regulatory protein AcoR promotes the expression of this operon using acetaldehyde, a cleavage product of acetoin, as its direct effector. Proposed model for 2,3-BD utilization in Pseudomonas aeruginosa strain PAO1 in downstream catabolic pathways, overview. Genes pa4148, pa4149, pa4150, pa4151, pa4152 and pa4153 comprise an operon responsible for 2,3-BD utilization, mutational analysis. Acetaldehyde is the direct inducer of the 2,3-BD utilization operon | Pseudomonas aeruginosa |
1.1.1.76 | physiological function | 2,3-butanediol (2,3-BD) is a primary microbial metabolite that enhances the virulence of Pseudomonas aeruginosa and alters the lung microbiome. 2,3-BD exists in three stereoisomeric forms: (2R,3R)-2,3-BD, meso-2,3-BD and (2S,3S)-2,3-BD | Pseudomonas aeruginosa |
2.3.1.190 | malfunction | acoA, acoB are knocked out individually in Pseudomonas aeruginosa PAO1. The mutants lose the ability to grow in acetoin or 2,3-butanediol | Pseudomonas aeruginosa |
2.3.1.190 | metabolism | the enzyme is involved in 2,3-butanediol catabolism | Pseudomonas aeruginosa |