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Literature summary extracted from

  • Bai, F.; Dai, L.; Fan, J.; Truong, N.; Rao, B.; Zhang, L.; Shen, Y.
    Engineered Serratia marcescens for efficient (3R)-acetoin and (2R,3R)-2,3-butanediol production (2015), J. Ind. Microbiol. Biotechnol., 42, 779-786.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.4 expressed in Escherichia coli BL21(DE3) cells Serratia marcescens
1.1.1.4 expressed in Serratia marcescens MG1 slaC mutant strain Bacillus subtilis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.4 35000
-
 x * 35000, SDS-PAGE Bacillus subtilis
1.1.1.4 35000
-
 x * 35000, SDS-PAGE Serratia marcescens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.4 (R)-acetoin + NADH + H+ Bacillus subtilis
-
 (R,R)-butane-2,3-diol + NAD+
-
 r
1.1.1.4 (R)-acetoin + NADH + H+ Serratia marcescens
-
 (R,R)-butane-2,3-diol + NAD+
-
 r
1.1.1.4 (R)-acetoin + NADH + H+ Bacillus subtilis 168
-
 (R,R)-butane-2,3-diol + NAD+
-
 r
1.1.1.4 (R)-acetoin + NADH + H+ Serratia marcescens MG1
-
 (R,R)-butane-2,3-diol + NAD+
-
 r
1.1.1.4 (R,R)-butane-2,3-diol + NAD+ Bacillus subtilis
-
 (R)-acetoin + NADH + H+
-
 r
1.1.1.4 (R,R)-butane-2,3-diol + NAD+ Serratia marcescens
-
 (R)-acetoin + NADH + H+
-
 r
1.1.1.4 (R,R)-butane-2,3-diol + NAD+ Bacillus subtilis 168
-
 (R)-acetoin + NADH + H+
-
 r
1.1.1.4 (R,R)-butane-2,3-diol + NAD+ Serratia marcescens MG1
-
 (R)-acetoin + NADH + H+
-
 r

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.4 Bacillus subtilis
-
-
-
1.1.1.4 Bacillus subtilis 168
-
-
-
1.1.1.4 Serratia marcescens
-
-
-
1.1.1.4 Serratia marcescens MG1
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.4 His-Trap column chromatography Bacillus subtilis
1.1.1.4 His-Trap column chromatography Serratia marcescens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.4 (R)-acetoin + NADH + H+
-
 Bacillus subtilis (R,R)-butane-2,3-diol + NAD+
-
 r
1.1.1.4 (R)-acetoin + NADH + H+
-
 Serratia marcescens (R,R)-butane-2,3-diol + NAD+
-
 r
1.1.1.4 (R)-acetoin + NADH + H+
-
 Bacillus subtilis 168 (R,R)-butane-2,3-diol + NAD+
-
 r
1.1.1.4 (R)-acetoin + NADH + H+
-
 Serratia marcescens MG1 (R,R)-butane-2,3-diol + NAD+
-
 r
1.1.1.4 (R,R)-butane-2,3-diol + NAD+
-
 Bacillus subtilis (R)-acetoin + NADH + H+
-
 r
1.1.1.4 (R,R)-butane-2,3-diol + NAD+
-
 Serratia marcescens (R)-acetoin + NADH + H+
-
 r
1.1.1.4 (R,R)-butane-2,3-diol + NAD+
-
 Bacillus subtilis 168 (R)-acetoin + NADH + H+
-
 r
1.1.1.4 (R,R)-butane-2,3-diol + NAD+
-
 Serratia marcescens MG1 (R)-acetoin + NADH + H+
-
 r
1.1.1.4 diacetyl + NADH + H+
-
 Bacillus subtilis (3S)-acetoin + NAD+
-
 r
1.1.1.4 diacetyl + NADH + H+
-
 Serratia marcescens (3S)-acetoin + NAD+
-
 r
1.1.1.4 diacetyl + NADH + H+
-
 Bacillus subtilis 168 (3S)-acetoin + NAD+
-
 r
1.1.1.4 diacetyl + NADH + H+
-
 Serratia marcescens MG1 (3S)-acetoin + NAD+
-
 r

Subunits

EC Number Subunits Comment Organism
1.1.1.4 ? x * 35000, SDS-PAGE Bacillus subtilis
1.1.1.4 ? x * 35000, SDS-PAGE Serratia marcescens

Synonyms

EC Number Synonyms Comment Organism
1.1.1.4 (2R,3R)-2,3-butanediol dehydrogenase
-
 Bacillus subtilis
1.1.1.4 BdhA
-
 Bacillus subtilis
1.1.1.4 meso-2,3-butanediol dehydrogenase
-
 Serratia marcescens
1.1.1.4 SLAC
-
 Serratia marcescens

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.4 NAD+
-
 Bacillus subtilis
1.1.1.4 NAD+
-
 Serratia marcescens
1.1.1.4 NADH
-
 Bacillus subtilis
1.1.1.4 NADH
-
 Serratia marcescens