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Literature summary extracted from

  • Wang, Y.; Tao, F.; Xu, P.
    Glycerol dehydrogenase plays a dual role in glycerol metabolism and 2,3-butanediol formation in Klebsiella pneumoniae (2014), J. Biol. Chem., 289, 6080-6090.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.4 expressed in Escherichia coli BL21(DE3) cells Klebsiella pneumoniae

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.4 4.15
-
 (3R,3S)-butane-2,3-diol enzyme GldA, at pH 9.0 and 30°C Klebsiella pneumoniae
1.1.1.4 4.94
-
 meso-butane-2,3-diol enzyme GldA, at pH 9.0 and 30°C Klebsiella pneumoniae
1.1.1.4 12.06
-
 (3R,3S)-butane-2,3-diol enzyme DhaD, at pH 9.0 and 30°C Klebsiella pneumoniae
1.1.1.4 14.49
-
 glycerol enzyme GldA, at pH 9.0 and 30°C Klebsiella pneumoniae
1.1.1.4 37.39
-
 meso-butane-2,3-diol enzyme DhaD, at pH 9.0 and 30°C Klebsiella pneumoniae
1.1.1.4 93.62
-
 glycerol enzyme DhaD, at pH 9.0 and 30°C Klebsiella pneumoniae
1.1.1.4 119.3
-
 (2R,3R)-butane-2,3-diol enzyme GldA, at pH 9.0 and 30°C Klebsiella pneumoniae
1.1.1.4 236.4
-
 (2R,3R)-butane-2,3-diol enzyme DhaD, at pH 9.0 and 30°C Klebsiella pneumoniae

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.4 45000
-
 x * 45000, SDS-PAGE Klebsiella pneumoniae

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.4 (2R,3R)-butane-2,3-diol + NAD+ Klebsiella pneumoniae
-
 (3R)-acetoin + NADH + H+
-
 ?
1.1.1.4 (2R,3R)-butane-2,3-diol + NAD+ Klebsiella pneumoniae ATCC 25955
-
 (3R)-acetoin + NADH + H+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.4 Klebsiella pneumoniae
-
-
-
1.1.1.4 Klebsiella pneumoniae ATCC 25955
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.4 HisTrap column chromatography and Sephadex G-25 gel filtration Klebsiella pneumoniae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.4 (2R,3R)-butane-2,3-diol + NAD+
-
 Klebsiella pneumoniae (3R)-acetoin + NADH + H+
-
 ?
1.1.1.4 (2R,3R)-butane-2,3-diol + NAD+
-
 Klebsiella pneumoniae ATCC 25955 (3R)-acetoin + NADH + H+
-
 ?
1.1.1.4 (3R,3S)-butane-2,3-diol + NAD+
-
 Klebsiella pneumoniae (3R,3S)-acetoin + NADH + H+
-
 ?
1.1.1.4 (3R,3S)-butane-2,3-diol + NAD+
-
 Klebsiella pneumoniae ATCC 25955 (3R,3S)-acetoin + NADH + H+
-
 ?
1.1.1.4 glycerol + NAD+
-
 Klebsiella pneumoniae ?
-
 ?
1.1.1.4 glycerol + NAD+
-
 Klebsiella pneumoniae ATCC 25955 ?
-
 ?
1.1.1.4 meso-butane-2,3-diol + NAD+
-
 Klebsiella pneumoniae (3S)-acetoin + NADH + H+
-
 ?
1.1.1.4 meso-butane-2,3-diol + NAD+
-
 Klebsiella pneumoniae ATCC 25955 (3S)-acetoin + NADH + H+
-
 ?
1.1.1.4 additional information no activity with (2S,3S)-butane-2,3-diol or diacetyl Klebsiella pneumoniae ?
-
 ?
1.1.1.4 additional information no activity with (2S,3S)-butane-2,3-diol or diacetyl Klebsiella pneumoniae ATCC 25955 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.1.4 ? x * 45000, SDS-PAGE Klebsiella pneumoniae

Synonyms

EC Number Synonyms Comment Organism
1.1.1.4 DHAD
-
 Klebsiella pneumoniae
1.1.1.4 GldA
-
 Klebsiella pneumoniae

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.1.1.4 3.68
-
 (3R,3S)-butane-2,3-diol enzyme DhaD, at pH 9.0 and 30°C Klebsiella pneumoniae
1.1.1.4 3.82
-
 (2R,3R)-butane-2,3-diol enzyme GldA, at pH 9.0 and 30°C Klebsiella pneumoniae
1.1.1.4 4.67
-
 meso-butane-2,3-diol enzyme DhaD, at pH 9.0 and 30°C Klebsiella pneumoniae
1.1.1.4 5.8
-
 (2R,3R)-butane-2,3-diol enzyme DhaD, at pH 9.0 and 30°C Klebsiella pneumoniae
1.1.1.4 10.38
-
 (3R,3S)-butane-2,3-diol enzyme GldA, at pH 9.0 and 30°C Klebsiella pneumoniae
1.1.1.4 10.51
-
 glycerol enzyme DhaD, at pH 9.0 and 30°C Klebsiella pneumoniae
1.1.1.4 15.33
-
 meso-butane-2,3-diol enzyme GldA, at pH 9.0 and 30°C Klebsiella pneumoniae
1.1.1.4 18.88
-
 glycerol enzyme GldA, at pH 9.0 and 30°C Klebsiella pneumoniae

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.4 NAD+
-
 Klebsiella pneumoniae

Expression

EC Number Organism Comment Expression
1.1.1.4 Klebsiella pneumoniae the enzyme gene dhaD is highly induced by glycerol up

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.1.1.4 0.03
-
 (2R,3R)-butane-2,3-diol enzyme DhaD, at pH 9.0 and 30°C Klebsiella pneumoniae
1.1.1.4 0.03
-
 (2R,3R)-butane-2,3-diol enzyme GldA, at pH 9.0 and 30°C Klebsiella pneumoniae
1.1.1.4 0.11
-
 glycerol enzyme DhaD, at pH 9.0 and 30°C Klebsiella pneumoniae
1.1.1.4 0.13
-
 meso-butane-2,3-diol enzyme DhaD, at pH 9.0 and 30°C Klebsiella pneumoniae
1.1.1.4 0.31
-
 (3R,3S)-butane-2,3-diol enzyme DhaD, at pH 9.0 and 30°C Klebsiella pneumoniae
1.1.1.4 1.3
-
 glycerol enzyme GldA, at pH 9.0 and 30°C Klebsiella pneumoniae
1.1.1.4 2.51
-
 (3R,3S)-butane-2,3-diol enzyme GldA, at pH 9.0 and 30°C Klebsiella pneumoniae
1.1.1.4 3.11
-
 meso-butane-2,3-diol enzyme GldA, at pH 9.0 and 30°C Klebsiella pneumoniae