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Literature summary extracted from

  • Ge, Y.; Li, K.; Li, L.; Gao, C.; Zhang, L.; Ma, C.; Xu, P.
    Contracted but effective: Production of enantiopure 2,3-butanediol by thermophilic and GRAS: Bacillus licheniformis (2016), Green Chem., 18, 4693-4703.
No PubMed abstract available

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.4 expressed in Escherichia coli BL21(DE3) cells Bacillus licheniformis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.4 (2R,3R)-butane-2,3-diol + NAD+ Bacillus licheniformis
-
 (R)-acetoin + NADH + H+
-
 r
1.1.1.4 (2R,3R)-butane-2,3-diol + NAD+ Bacillus licheniformis MW3
-
 (R)-acetoin + NADH + H+
-
 r
1.1.1.4 (R)-acetoin + NADH + H+ Bacillus licheniformis
-
 (2R,3R)-butane-2,3-diol + NAD+
-
 r
1.1.1.4 (R)-acetoin + NADH + H+ Bacillus licheniformis MW3
-
 (2R,3R)-butane-2,3-diol + NAD+
-
 r

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.4 Bacillus licheniformis
-
-
-
1.1.1.4 Bacillus licheniformis MW3
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.4 HisTrap column chromatography Bacillus licheniformis

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.4 0.004
-
 with (2S,3s)-butane-2,3-diol as substrate, at pH 7.4 and 30°C Bacillus licheniformis
1.1.1.4 0.005
-
 with pentane-1,2-diol as substrate, at pH 7.4 and 30°C Bacillus licheniformis
1.1.1.4 0.007
-
 with butanone as substrate, at pH 7.4 and 30°C Bacillus licheniformis
1.1.1.4 0.01
-
 with pentane-2,3-dione as substrate, at pH 7.4 and 30°C Bacillus licheniformis
1.1.1.4 0.012
-
 with hexane-2,5-dione as substrate, at pH 7.4 and 30°C Bacillus licheniformis
1.1.1.4 0.019
-
 with pentane-2,4-dione as substrate, at pH 7.4 and 30°C Bacillus licheniformis
1.1.1.4 0.044
-
 with n-butyöaödehyde as substrate, at pH 7.4 and 30°C Bacillus licheniformis
1.1.1.4 0.048
-
 with butane-1,2-diol as substrate, at pH 7.4 and 30°C Bacillus licheniformis
1.1.1.4 0.084
-
 with diacetyl as substrate, at pH 7.4 and 30°C Bacillus licheniformis
1.1.1.4 0.093
-
 with propane-1,2-diol as substrate, at pH 7.4 and 30°C Bacillus licheniformis
1.1.1.4 0.257
-
 with 1-hydroxy-2-butanone as substrate, at pH 7.4 and 30°C Bacillus licheniformis
1.1.1.4 0.732
-
 with 1,3-dihydroxyacetone as substrate, at pH 7.4 and 30°C Bacillus licheniformis
1.1.1.4 1.056
-
 with (2R,3R)-butane-2,3-diol as substrate, at pH 7.4 and 30°C Bacillus licheniformis
1.1.1.4 1.193
-
 with (R)-acetoin as substrate, at pH 7.4 and 30°C Bacillus licheniformis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.4 (2R,3R)-butane-2,3-diol + NAD+
-
 Bacillus licheniformis (R)-acetoin + NADH + H+
-
 r
1.1.1.4 (2R,3R)-butane-2,3-diol + NAD+
-
 Bacillus licheniformis MW3 (R)-acetoin + NADH + H+
-
 r
1.1.1.4 (2S,3S)-butane-2,3-diol + NAD+ very low activity Bacillus licheniformis (2S)-acetoin + NADH + H+
-
 ?
1.1.1.4 (2S,3S)-butane-2,3-diol + NAD+ very low activity Bacillus licheniformis MW3 (2S)-acetoin + NADH + H+
-
 ?
1.1.1.4 (R)-acetoin + NADH + H+
-
 Bacillus licheniformis (2R,3R)-butane-2,3-diol + NAD+
-
 r
1.1.1.4 (R)-acetoin + NADH + H+
-
 Bacillus licheniformis MW3 (2R,3R)-butane-2,3-diol + NAD+
-
 r
1.1.1.4 1,3-dihydroxyacetone + NADH + H+ low activity Bacillus licheniformis ?
-
 ?
1.1.1.4 1-hydroxy-2-butanone + NADH + H+ low activity Bacillus licheniformis ?
-
 ?
1.1.1.4 butane-1,2-diol + NAD+ low activity Bacillus licheniformis ?
-
 ?
1.1.1.4 butanone + NADH + H+ low activity Bacillus licheniformis ?
-
 ?
1.1.1.4 diacetyl + NADH + H+ low activity Bacillus licheniformis acetoin + NAD+
-
 ?
1.1.1.4 diacetyl + NADH + H+ low activity Bacillus licheniformis MW3 acetoin + NAD+
-
 ?
1.1.1.4 hexane-2,5-dione + NADH + H+ low activity Bacillus licheniformis ?
-
 ?
1.1.1.4 additional information no activity with meso-butane-2,3-diol, glycerol, glycerol-3-phosphate, 1,3-dihydroxyacetone, dihydroxyacetonephosphate, glyceraldehyde-3-phosphate, 2-butanol, and 1,3-butanediol Bacillus licheniformis ?
-
 ?
1.1.1.4 additional information no activity with meso-butane-2,3-diol, glycerol, glycerol-3-phosphate, 1,3-dihydroxyacetone, dihydroxyacetonephosphate, glyceraldehyde-3-phosphate, 2-butanol, and 1,3-butanediol Bacillus licheniformis MW3 ?
-
 ?
1.1.1.4 n-butylaldehyde + NADH + H+ low activity Bacillus licheniformis ?
-
 ?
1.1.1.4 pentane-1,2-diol + NAD+ very low activity Bacillus licheniformis ?
-
 ?
1.1.1.4 pentane-2,3-dione + NADH + H+ low activity Bacillus licheniformis ?
-
 ?
1.1.1.4 pentane-2,4-dione + NADH + H+ low activity Bacillus licheniformis ?
-
 ?
1.1.1.4 propane-1,2-diol + NAD+ low activity Bacillus licheniformis ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.1.1.4 2R,3R-2,3-BD dehydrogenase
-
 Bacillus licheniformis
1.1.1.4 2R-3R-BDH
-
 Bacillus licheniformis
1.1.1.4 GDH
-
 Bacillus licheniformis

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.4 NAD+
-
 Bacillus licheniformis