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Literature summary extracted from

  • Xu, G.C.; Bian, Y.Q.; Han, R.Z.; Dong, J.J.; Ni, Y.
    Cloning, expression, and characterization of budC gene encoding meso-2,3-butanediol dehydrogenase from Bacillus licheniformis (2016), Appl. Biochem. Biotechnol., 178, 604-617.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.76 expressed in Escherichia coli BL21(DE3) cells Bacillus licheniformis
1.1.1.304 expression in Escherichia coli Bacillus licheniformis

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.76 Ag+ strong inhibition Bacillus licheniformis
1.1.1.76 Al3+ strong inhibition Bacillus licheniformis
1.1.1.76 Cu2+ strong inhibition Bacillus licheniformis
1.1.1.76 Fe3+ strong inhibition Bacillus licheniformis
1.1.1.76 K+ slight inhibition Bacillus licheniformis
1.1.1.76 additional information not inhibited by EDTA Bacillus licheniformis
1.1.1.76 Zn2+ slight inhibition Bacillus licheniformis
1.1.1.304 Ag+ 1 mM, 0.5% of initial activity with substrate diacetyl, 0.5% with substrate 2,3-butanediol, respectively Bacillus licheniformis
1.1.1.304 Al3+ 1 mM, 4% of initial activity with substrate diacetyl, 6% with substrate 2,3-butanediol, respectively Bacillus licheniformis
1.1.1.304 Cu2+ 1 mM, 0.2% of initial activity with substrate diacetyl, 1% with substrate 2,3-butanediol, respectively Bacillus licheniformis
1.1.1.304 EDTA 1 mM, 91% of initial activity with substrate diacetyl, 90% with substrate 2,3-butanediol, respectively Bacillus licheniformis
1.1.1.304 Fe3+ 1 mM, 2% of initial activity with substrate diacetyl, 3.5% with substrate 2,3-butanediol, respectively Bacillus licheniformis
1.1.1.304 Zn2+ 1 mM, 75% of initial activity with substrate diacetyl, 80% with substrate 2,3-butanediol, respectively Bacillus licheniformis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.76 0.47
-
(2S)-acetoin at pH 10.0 and 30°C Bacillus licheniformis
1.1.1.76 7.25
-
(R,R)-butane-2,3-diol at pH 10.0 and 30°C Bacillus licheniformis
1.1.1.76 72.4
-
diacetyl at pH 5.0 and 30°C Bacillus licheniformis
1.1.1.304 0.25
-
NADH pH 6.0, 30°C Bacillus licheniformis
1.1.1.304 0.34
-
NAD+ pH 10.0, 30°C Bacillus licheniformis
1.1.1.304 0.47
-
(3S)-acetoin pH 6.0, 30°C Bacillus licheniformis
1.1.1.304 7.25
-
(2S,3S)-2,3-butanediol pH 10.0, 30°C Bacillus licheniformis
1.1.1.304 72.4
-
diacetyl pH 6.0, 30°C Bacillus licheniformis

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.1.1.76 Co2+ slight activation Bacillus licheniformis
1.1.1.76 Mn2+ slight activation Bacillus licheniformis
1.1.1.76 additional information not influenced by Ni2+, Ba2+, Li+, Ca2+, and Na+ Bacillus licheniformis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.76 30000
-
4 * 30000, SDS-PAGE Bacillus licheniformis
1.1.1.76 125000
-
gel filtration Bacillus licheniformis
1.1.1.304 30000
-
4 * 30000, SDS-PAGE Bacillus licheniformis
1.1.1.304 125000
-
gel filtration Bacillus licheniformis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.76 (2S,3S)-butane-2,3-diol + NAD+ Bacillus licheniformis 100% activity (2S)-acetoin + NADH + H+
-
r

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.76 Bacillus licheniformis
-
-
-
1.1.1.304 Bacillus licheniformis
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.76 HisTrap column chromatography and Superdex 200 gel filtration Bacillus licheniformis

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.304 120
-
substrate diacetyl, 30°C, pH 6.0 Bacillus licheniformis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.76 (2S)-acetoin + NADH + H+ 97% activity compared to diacetyl Bacillus licheniformis (2S,3S)-butane-2,3-diol + NAD+
-
r
1.1.1.76 (2S,3S)-butane-2,3-diol + NAD+ 100% activity Bacillus licheniformis (2S)-acetoin + NADH + H+
-
r
1.1.1.76 (R,R)-butane-2,3-diol + NAD+
-
Bacillus licheniformis ?
-
?
1.1.1.76 1,2-propanediol + NAD+ 0.57% activity compared to (2S,3S)-butane-2,3-diol Bacillus licheniformis ?
-
?
1.1.1.76 2,3-hexanedione + NADH + H+ 66% activity compared to diacetyl Bacillus licheniformis ?
-
?
1.1.1.76 2,3-pentanedione + NADH + H+ 69% activity compared to diacetyl Bacillus licheniformis ?
-
?
1.1.1.76 3,4-hexanedione + NADH + H+ 10% activity compared to diacetyl Bacillus licheniformis ?
-
?
1.1.1.76 diacetyl + NADH + H+ 100% activity Bacillus licheniformis (2S)-acetoin + NAD+
-
?
1.1.1.76 additional information the enzyme shows no activity toward racemic acetoin in the presence of NAD+ as well as no activity with NADPH, 1,4-butanediol, 2,5-hexanedione, 2,4-pentanedione, 2-butanone, methanol, mannitol, and glycerol Bacillus licheniformis ?
-
?
1.1.1.304 (2S,3S)-2,3-butanediol + NAD+
-
Bacillus licheniformis (3S)-acetoin + NADH + H+
-
r
1.1.1.304 (3S)-acetoin + NADH + H+ 97% of the activity with diacetyl Bacillus licheniformis (2S,3S)-2,3-butanediol + NAD+ 97.3% enantiomeric excess and 96.5% diastereomeric excess r
1.1.1.304 1,2-propanediol + NAD+
-
Bacillus licheniformis ? + NADH + H+ 0.5% of the activity with 2,3-butanediol r
1.1.1.304 2,3-hexanedione + NADH + H+
-
Bacillus licheniformis ? + NAD+ 66% of the activity with diacetyl ?
1.1.1.304 2,3-pentanedione + NADH + H+
-
Bacillus licheniformis ? + NAD+ 69% of the activity with diacetyl ?
1.1.1.304 3,4-hexanedione + NADH + H+
-
Bacillus licheniformis ? + NAD+ 10% of the activity with diacetyl ?
1.1.1.304 diacetyl + NADH + H+
-
Bacillus licheniformis (3S)-acetoin + NAD+ 97.3% enantiomeric excess ir
1.1.1.304 additional information enzyme shows oxidative activity to racemic 2,3-butanediol but no activity toward racemic acetoin in the presence of NAD+ Bacillus licheniformis ?
-
?

Subunits

EC Number Subunits Comment Organism
1.1.1.76 homotetramer 4 * 30000, SDS-PAGE Bacillus licheniformis
1.1.1.304 tetramer 4 * 30000, SDS-PAGE Bacillus licheniformis

Synonyms

EC Number Synonyms Comment Organism
1.1.1.76 BDH
-
Bacillus licheniformis
1.1.1.76 budC
-
Bacillus licheniformis
1.1.1.76 meso-2,3-butanediol dehydrogenase
-
Bacillus licheniformis
1.1.1.304 budC
-
Bacillus licheniformis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.1.76 37
-
-
Bacillus licheniformis

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.1.1.76 50
-
at 50°C, the enzymatic activity drops rapidly to half of its initial activity within 40 min, then further decreases to 20% after 330 min Bacillus licheniformis

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.1.1.76 202
-
(2S)-acetoin at pH 10.0 and 30°C Bacillus licheniformis
1.1.1.76 591
-
(R,R)-butane-2,3-diol at pH 10.0 and 30°C Bacillus licheniformis
1.1.1.76 1222
-
diacetyl at pH 5.0 and 30°C Bacillus licheniformis
1.1.1.304 202
-
(3S)-acetoin pH 6.0, 30°C Bacillus licheniformis
1.1.1.304 591
-
(2S,3S)-2,3-butanediol pH 10.0, 30°C Bacillus licheniformis
1.1.1.304 748
-
NAD+ pH 10.0, 30°C Bacillus licheniformis
1.1.1.304 1222
-
diacetyl pH 6.0, 30°C Bacillus licheniformis
1.1.1.304 1274
-
NADH pH 6.0, 30°C Bacillus licheniformis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.76 5
-
for diacetyl reduction Bacillus licheniformis
1.1.1.76 10
-
for 2,3-butanediol oxidation Bacillus licheniformis
1.1.1.304 5
-
reduction of diacetyl Bacillus licheniformis
1.1.1.304 10
-
oxidation of butanediol Bacillus licheniformis

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.1.1.76 4.5 10 the enzyme shows a relatively high activity over a wide pH range from 5.0 to 8.0 for racemic acetoin reduction and more than 60% of activity is retained between pH 4.5 and 10.0 Bacillus licheniformis
1.1.1.304 5 8 reduction of diacetyl Bacillus licheniformis

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.76 NAD+
-
Bacillus licheniformis
1.1.1.76 NADH
-
Bacillus licheniformis
1.1.1.304 additional information no activity with NADPH Bacillus licheniformis
1.1.1.304 NAD+
-
Bacillus licheniformis
1.1.1.304 NADH
-
Bacillus licheniformis

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.1.1.76 16.9
-
diacetyl at pH 5.0 and 30°C Bacillus licheniformis
1.1.1.76 81.5
-
(R,R)-butane-2,3-diol at pH 10.0 and 30°C Bacillus licheniformis
1.1.1.76 432
-
(2S)-acetoin at pH 10.0 and 30°C Bacillus licheniformis
1.1.1.304 16.9
-
diacetyl pH 6.0, 30°C Bacillus licheniformis
1.1.1.304 81.5
-
(2S,3S)-2,3-butanediol pH 10.0, 30°C Bacillus licheniformis
1.1.1.304 432
-
(3S)-acetoin pH 6.0, 30°C Bacillus licheniformis
1.1.1.304 2192
-
NAD+ pH 10.0, 30°C Bacillus licheniformis
1.1.1.304 5072
-
NADH pH 6.0, 30°C Bacillus licheniformis