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Literature summary extracted from

  • Miao, X.; Huang, X.; Zhang, G.; Zhao, X.; Zhu, X.; Dong, H.
    Crystallization and preliminary X-ray study of a (2R,3R)-2,3-butanediol dehydrogenase from Bacillus coagulans 2-6 (2013), Acta Crystallogr. Sect. F, 69, 1140-1142.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.4 expressed in Escherichia coli BL21(DE3) cells Weizmannia coagulans

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.1.1.4 hanging drop vapor diffusion method, using 8% (v/v) Tacsimate pH 4.6, 18% (w/v) polyethylene glycol 3350 Weizmannia coagulans

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.1.1.4 Zn2+ dependent on Weizmannia coagulans

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.4 38500
-
 x * 38500, calculated from amino acid sequence Weizmannia coagulans
1.1.1.4 40000
-
 x * 40000, SDS-PAGE Weizmannia coagulans

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.4 (R,R)-butane-2,3-diol + NAD+ Weizmannia coagulans
-
 (R)-acetoin + NADH + H+
-
 ?
1.1.1.4 (R,R)-butane-2,3-diol + NAD+ Weizmannia coagulans 2-6
-
 (R)-acetoin + NADH + H+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.4 Weizmannia coagulans
-
-
-
1.1.1.4 Weizmannia coagulans 2-6
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.4 Ni2+-chelating affinity column chromatography and Superdex 200 gel filtration Weizmannia coagulans

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.4 (R,R)-butane-2,3-diol + NAD+
-
 Weizmannia coagulans (R)-acetoin + NADH + H+
-
 ?
1.1.1.4 (R,R)-butane-2,3-diol + NAD+
-
 Weizmannia coagulans 2-6 (R)-acetoin + NADH + H+
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.1.4 ? x * 40000, SDS-PAGE Weizmannia coagulans
1.1.1.4 ? x * 38500, calculated from amino acid sequence Weizmannia coagulans

Synonyms

EC Number Synonyms Comment Organism
1.1.1.4 (2R,3R)-2,3-butanediol dehydrogenase
-
 Weizmannia coagulans
1.1.1.4 R,R-BDH
-
 Weizmannia coagulans

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.4 NAD+
-
 Weizmannia coagulans