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Literature summary extracted from

  • Gao, J.; Yang, H.H.; Feng, X.H.; Li, S.; Xu, H.
    A 2,3-butanediol dehydrogenase from Paenibacillus polymyxa ZJ-9 for mainly producing R,R-2,3-butanediol: purification, characterization and cloning (2013), J. Basic Microbiol., 53, 733-741.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.4 DNA and amino acid sequence determination and analysis, sequence comparison, subcloning in Escherichia coli strain JM109, expression in Escherichia coli strain BL21 (DE3) Paenibacillus polymyxa

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.4 0.2
-
 (3R,3S)-acetoin pH 7.0, 30°C Paenibacillus polymyxa
1.1.1.4 0.32
-
 NADH pH 7.0, 30°C Paenibacillus polymyxa
1.1.1.4 0.84
-
 acetoin (S)-acetoin, pH 7.0, 30°C Paenibacillus polymyxa
1.1.1.4 2.3
-
 NAD+ pH 7.0, 30°C Paenibacillus polymyxa
1.1.1.4 2.73
-
 meso-butane-2,3-diol pH 7.0, 30°C Paenibacillus polymyxa
1.1.1.4 7.67
-
 (2R,3R)-butane-2,3-diol pH 7.0, 30°C Paenibacillus polymyxa

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.4 44500
-
 2 * 44500, SDS-PAGE Paenibacillus polymyxa
1.1.1.4 90000
-
 gel filtration Paenibacillus polymyxa

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.4 Paenibacillus polymyxa
-
 isolated from soil
-
1.1.1.4 Paenibacillus polymyxa ZJ-9
-
 isolated from soil
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.4 native enzyme 35fold to homogeneity by fractional ammonium sulfate precipitation, followed by two different steps of anion exchange chromatography Paenibacillus polymyxa

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.1.1.4 cell culture
-
 Paenibacillus polymyxa
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.4 4.65
-
 purified native enzyme, pH 7.0, 30°C Paenibacillus polymyxa

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.4 (2R,3R)-butane-2,3-diol + NAD+
-
 Paenibacillus polymyxa (3R,3S)-acetoin + NADH + H+
-
 r
1.1.1.4 (2R,3R)-butane-2,3-diol + NAD+
-
 Paenibacillus polymyxa ZJ-9 (3R,3S)-acetoin + NADH + H+
-
 r
1.1.1.4 meso-butane-2,3-diol + NAD+
-
 Paenibacillus polymyxa acetoin + NADH + H+
-
 r
1.1.1.4 meso-butane-2,3-diol + NAD+
-
 Paenibacillus polymyxa ZJ-9 acetoin + NADH + H+
-
 r

Subunits

EC Number Subunits Comment Organism
1.1.1.4 dimer 2 * 44500, SDS-PAGE Paenibacillus polymyxa

Synonyms

EC Number Synonyms Comment Organism
1.1.1.4 2,3-butanediol dehydrogenase
-
 Paenibacillus polymyxa

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.1.4 30
-
-
 Paenibacillus polymyxa

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.4 6
-
 oxidation reaction Paenibacillus polymyxa
1.1.1.4 8
-
 reduction reaction Paenibacillus polymyxa

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.4 additional information no activity with NADP+ or NADPH as the coenzyme, respectively Paenibacillus polymyxa
1.1.1.4 NAD+
-
 Paenibacillus polymyxa
1.1.1.4 NADH
-
 Paenibacillus polymyxa