EC Number | Cloned (Comment) | Organism |
---|---|---|
1.1.1.B20 | gene budC, expression in Escherichia coli strain BL21(DE3) pLys | Klebsiella pneumoniae |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.1.1.B20 | 0.05 | - |
NADH | pH 8.0-9.0, 35°C, recombinant enzyme | Klebsiella pneumoniae | |
1.1.1.B20 | 0.65 | - |
(3R)-acetoin | pH 8.0-9.0, 35°C, recombinant enzyme | Klebsiella pneumoniae | |
1.1.1.B20 | 0.66 | - |
NAD+ | pH 8.0-9.0, 35°C, recombinant enzyme | Klebsiella pneumoniae | |
1.1.1.B20 | 6 | - |
4-methyl-2-pentanone | pH 8.0-9.0, 35°C, recombinant enzyme | Klebsiella pneumoniae | |
1.1.1.B20 | 13 | - |
meso-2,3-butanediol | pH 8.0-9.0, 35°C, recombinant enzyme | Klebsiella pneumoniae |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.1.1.B20 | (3R)-acetoin + NADH + H+ | Klebsiella pneumoniae | - |
meso-2,3-butanediol + NAD+ | - |
r | |
1.1.1.B20 | (3R)-acetoin + NADH + H+ | Klebsiella pneumoniae XJ-Li | - |
meso-2,3-butanediol + NAD+ | - |
r |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.1.1.B20 | Klebsiella pneumoniae | - |
gene budC | - |
1.1.1.B20 | Klebsiella pneumoniae XJ-Li | - |
gene budC | - |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
1.1.1.B20 | 21 | - |
purified reconstituted, recombinant enzyme, reduction of 4-methyl-2-pentanone, pH 8.0, 35°C | Klebsiella pneumoniae |
1.1.1.B20 | 66 | - |
purified reconstituted, recombinant enzyme, oxidation of meso-2,3-butanediol, pH 8.0, 35°C | Klebsiella pneumoniae |
1.1.1.B20 | 218 | - |
purified reconstituted, recombinant enzyme, reduction of acetoin, pH 8.0, 35°C | Klebsiella pneumoniae |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.1.1.B20 | (3R)-acetoin + NADH + H+ | - |
Klebsiella pneumoniae | meso-2,3-butanediol + NAD+ | - |
r | |
1.1.1.B20 | (3R)-acetoin + NADH + H+ | - |
Klebsiella pneumoniae XJ-Li | meso-2,3-butanediol + NAD+ | - |
r | |
1.1.1.B20 | 4-methyl-2-pentanone + NADH + H+ | low activity | Klebsiella pneumoniae | 4-methyl-2-pentanol + NAD+ | - |
r | |
1.1.1.B20 | 4-methyl-2-pentanone + NADH + H+ | low activity | Klebsiella pneumoniae XJ-Li | 4-methyl-2-pentanol + NAD+ | - |
r | |
1.1.1.B20 | additional information | the enzyme is highly stereospecific, and shows no significant activities towards 2R,3R-2,3-butanediol, 1,4-butanediol, and 2S,3S-2,3-butanediol | Klebsiella pneumoniae | ? | - |
? | |
1.1.1.B20 | additional information | the enzyme is highly stereospecific, and shows no significant activities towards 2R,3R-2,3-butanediol, 1,4-butanediol, and 2S,3S-2,3-butanediol | Klebsiella pneumoniae XJ-Li | ? | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.1.1.B20 | meso-2,3-BDH | - |
Klebsiella pneumoniae |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.1.1.B20 | 35 | - |
- |
Klebsiella pneumoniae |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.1.1.B20 | 8 | 9 | - |
Klebsiella pneumoniae |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.1.1.B20 | NAD+ | - |
Klebsiella pneumoniae | |
1.1.1.B20 | NADH | - |
Klebsiella pneumoniae |
EC Number | General Information | Comment | Organism |
---|---|---|---|
1.1.1.B20 | evolution | the enzyme belongs to the short chain dehydrogenase/reductase family | Klebsiella pneumoniae |