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Literature summary extracted from

  • Zhang, G.L.; Wang, C.W.; Li, C.
    Cloning, expression and characterization of meso-2,3-butanediol dehydrogenase from Klebsiella pneumoniae (2012), Biotechnol. Lett., 34, 1519-1523.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.B20 gene budC, expression in Escherichia coli strain BL21(DE3) pLys Klebsiella pneumoniae

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.B20 0.05
-
NADH pH 8.0-9.0, 35°C, recombinant enzyme Klebsiella pneumoniae
1.1.1.B20 0.65
-
(3R)-acetoin pH 8.0-9.0, 35°C, recombinant enzyme Klebsiella pneumoniae
1.1.1.B20 0.66
-
NAD+ pH 8.0-9.0, 35°C, recombinant enzyme Klebsiella pneumoniae
1.1.1.B20 6
-
4-methyl-2-pentanone pH 8.0-9.0, 35°C, recombinant enzyme Klebsiella pneumoniae
1.1.1.B20 13
-
meso-2,3-butanediol pH 8.0-9.0, 35°C, recombinant enzyme Klebsiella pneumoniae

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.B20 (3R)-acetoin + NADH + H+ Klebsiella pneumoniae
-
meso-2,3-butanediol + NAD+
-
r
1.1.1.B20 (3R)-acetoin + NADH + H+ Klebsiella pneumoniae XJ-Li
-
meso-2,3-butanediol + NAD+
-
r

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.B20 Klebsiella pneumoniae
-
gene budC
-
1.1.1.B20 Klebsiella pneumoniae XJ-Li
-
gene budC
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.B20 21
-
purified reconstituted, recombinant enzyme, reduction of 4-methyl-2-pentanone, pH 8.0, 35°C Klebsiella pneumoniae
1.1.1.B20 66
-
purified reconstituted, recombinant enzyme, oxidation of meso-2,3-butanediol, pH 8.0, 35°C Klebsiella pneumoniae
1.1.1.B20 218
-
purified reconstituted, recombinant enzyme, reduction of acetoin, pH 8.0, 35°C Klebsiella pneumoniae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.B20 (3R)-acetoin + NADH + H+
-
Klebsiella pneumoniae meso-2,3-butanediol + NAD+
-
r
1.1.1.B20 (3R)-acetoin + NADH + H+
-
Klebsiella pneumoniae XJ-Li meso-2,3-butanediol + NAD+
-
r
1.1.1.B20 4-methyl-2-pentanone + NADH + H+ low activity Klebsiella pneumoniae 4-methyl-2-pentanol + NAD+
-
r
1.1.1.B20 4-methyl-2-pentanone + NADH + H+ low activity Klebsiella pneumoniae XJ-Li 4-methyl-2-pentanol + NAD+
-
r
1.1.1.B20 additional information the enzyme is highly stereospecific, and shows no significant activities towards 2R,3R-2,3-butanediol, 1,4-butanediol, and 2S,3S-2,3-butanediol Klebsiella pneumoniae ?
-
?
1.1.1.B20 additional information the enzyme is highly stereospecific, and shows no significant activities towards 2R,3R-2,3-butanediol, 1,4-butanediol, and 2S,3S-2,3-butanediol Klebsiella pneumoniae XJ-Li ?
-
?

Synonyms

EC Number Synonyms Comment Organism
1.1.1.B20 meso-2,3-BDH
-
Klebsiella pneumoniae

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.1.B20 35
-
-
Klebsiella pneumoniae

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.B20 8 9
-
Klebsiella pneumoniae

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.B20 NAD+
-
Klebsiella pneumoniae
1.1.1.B20 NADH
-
Klebsiella pneumoniae

General Information

EC Number General Information Comment Organism
1.1.1.B20 evolution the enzyme belongs to the short chain dehydrogenase/reductase family Klebsiella pneumoniae