EC Number | Cloned (Comment) | Organism |
---|---|---|
1.1.1.4 | gene mbd1, DNA and amino acid sequence determination and analysis, phylogenetic analysis | Mycobacterium sp. |
1.1.1.4 | phylogenetic analysis | Mycolicibacterium smegmatis |
1.1.1.4 | phylogenetic analysis | Mycolicibacterium vanbaalenii |
1.1.1.4 | phylogenetic analysis | Mycolicibacterium gilvum |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
1.1.1.4 | 5,5'-dithiobis(2-nitrobenzoic acid) | - |
Mycobacterium sp. | |
1.1.1.4 | Cu2+ | - |
Mycobacterium sp. | |
1.1.1.4 | EDTA | - |
Mycobacterium sp. | |
1.1.1.4 | Hg2+ | - |
Mycobacterium sp. | |
1.1.1.4 | Zn2+ | - |
Mycobacterium sp. |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.1.1.4 | 0.06 | - |
(3R,3S)-acetoin | pH 7.0, 30°C | Mycobacterium sp. |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.1.1.4 | (2R,3R)-butane-2,3-diol + NAD+ | Mycobacterium sp. | preferred substrate | (3R,3S)-acetoin + NADH + H+ | - |
r | |
1.1.1.4 | rac-1,2-propanediol + NAD+ | Mycobacterium sp. | - |
hydroxyacetone + NADH + H+ | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.1.1.4 | Mycobacterium sp. | F1T242 | gene mbd1 | - |
1.1.1.4 | Mycolicibacterium gilvum | - |
- |
- |
1.1.1.4 | Mycolicibacterium smegmatis | - |
- |
- |
1.1.1.4 | Mycolicibacterium vanbaalenii | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.1.1.4 | native enzyme by anion exchange and hydrophobic interaction chromatography, followed by ultrafiltration and native PAGE | Mycobacterium sp. |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.1.1.4 | (2R,3R)-butane-2,3-diol + NAD+ | preferred substrate | Mycobacterium sp. | (3R,3S)-acetoin + NADH + H+ | - |
r | |
1.1.1.4 | (2R,3R)-butane-2,3-diol + NAD+ | preferred substrate, very low activity with the (S)-enantiomer | Mycobacterium sp. | (3R,3S)-acetoin + NADH + H+ | - |
r | |
1.1.1.4 | (R)-1,2-propanediol + NAD+ | 73% activity compared to (2R,3R)-butane-2,3-diol in the oxidation reaction, very low activity with the (S)-enantiomer | Mycobacterium sp. | hydroxyacetone + NADH + H+ | - |
? | |
1.1.1.4 | 1,2-hexanediol + NAD+ | 50% activity compared to (2R,3R)-butane-2,3-diol in the oxidation reaction | Mycobacterium sp. | ? + NADH + H+ | - |
? | |
1.1.1.4 | 1,2-pentanediol + NAD+ | 74% activity compared to (2R,3R)-butane-2,3-diol in the oxidation reaction | Mycobacterium sp. | ? + NADH + H+ | - |
? | |
1.1.1.4 | meso-butane-2,3-diol + NAD+ | 61% activity compared to (2R,3R)-butane-2,3-diol in the oxidation reaction | Mycobacterium sp. | acetoin + NADH + H+ | - |
r | |
1.1.1.4 | additional information | substrate specificity, overview. No or poor activity with ethanol, ethylene glycol, diethylene glycol, 1-propanol, 2-propanol, 1,3-propanediol, glycerol, dipropylene glycol, 1-butanol, 1,4-butanediol, 1,2,4-butanetriol, (2S,3S)-butane-2,3-diol, 1,5-pentanediol, 2,4-pentanediol, 3-methyl-1,5-pentandiol, and 1,2,6-hexantriol | Mycobacterium sp. | ? | - |
? | |
1.1.1.4 | rac-1,2-propanediol + NAD+ | - |
Mycobacterium sp. | hydroxyacetone + NADH + H+ | - |
? | |
1.1.1.4 | rac-1,2-propanediol + NAD+ | 68% activity compared to (2R,3R)-butane-2,3-diol in the oxidation reaction | Mycobacterium sp. | hydroxyacetone + NADH + H+ | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.1.1.4 | ? | x * 36430, sequence calculation | Mycobacterium sp. |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.1.1.4 | (2R,3R)-2,3-butanediol dehydrogenase | - |
Mycolicibacterium smegmatis |
1.1.1.4 | (2R,3R)-2,3-butanediol dehydrogenase | - |
Mycolicibacterium vanbaalenii |
1.1.1.4 | (2R,3R)-2,3-butanediol dehydrogenase | - |
Mycolicibacterium gilvum |
1.1.1.4 | (2R,3R)-2,3-butanediol dehydrogenase | - |
Mycobacterium sp. |
1.1.1.4 | R,R-BD-DH | - |
Mycolicibacterium smegmatis |
1.1.1.4 | R,R-BD-DH | - |
Mycolicibacterium vanbaalenii |
1.1.1.4 | R,R-BD-DH | - |
Mycolicibacterium gilvum |
1.1.1.4 | R,R-BD-DH | - |
Mycobacterium sp. |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.1.1.4 | 85 | - |
- |
Mycobacterium sp. |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.1.1.4 | 50 | - |
rapid denaturation of the purified enzyme above 50°C | Mycobacterium sp. |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.1.1.4 | 7 | - |
assay at, reduction reaction | Mycobacterium sp. |
1.1.1.4 | 7.5 | - |
assay at, oxidation reaction | Mycobacterium sp. |
EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|---|
1.1.1.4 | 5 | 8.5 | stable, rapid denaturation below pH 5.0 | Mycobacterium sp. |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.1.1.4 | NAD+ | - |
Mycobacterium sp. | |
1.1.1.4 | NADH | - |
Mycobacterium sp. |
EC Number | Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|---|
1.1.1.4 | Mycobacterium sp. | isoelectric focussing | - |
4.9 |
EC Number | General Information | Comment | Organism |
---|---|---|---|
1.1.1.4 | evolution | the enzyme has homology to the medium-chain dehydrogenases/reductases with preference for secondary alcohols, phylogenetic analysis | Mycolicibacterium smegmatis |
1.1.1.4 | evolution | the enzyme has homology to the medium-chain dehydrogenases/reductases with preference for secondary alcohols, phylogenetic analysis | Mycolicibacterium vanbaalenii |
1.1.1.4 | evolution | the enzyme has homology to the medium-chain dehydrogenases/reductases with preference for secondary alcohols, phylogenetic analysis | Mycolicibacterium gilvum |
1.1.1.4 | evolution | the enzyme has homology to the medium-chain dehydrogenases/reductases with preference for secondary alcohols, phylogenetic analysis | Mycobacterium sp. |