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Literature summary extracted from

  • Gonzalez, E.; Fernandez, M.R.; Marco, D.; Calam, E.; Sumoy, L.; Pares, X.; Dequin, S.; Biosca, J.A.
    Role of Saccharomyces cerevisiae oxidoreductases Bdh1p and Ara1p in the metabolism of acetoin and 2,3-butanediol (2010), Appl. Environ. Microbiol., 76, 670-679.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.427 expressed in Escherichia coli as a His-tagged fusion protein Saccharomyces cerevisiae

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.4 Saccharomyces cerevisiae
-
-
-
1.1.1.B20 Saccharomyces cerevisiae
-
-
-
1.1.1.76 Saccharomyces cerevisiae
-
-
-
1.1.1.427 Saccharomyces cerevisiae
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.427 partial Saccharomyces cerevisiae
1.1.1.427 using Ni-NTA chromatography Saccharomyces cerevisiae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.4 (2R,3R)-2,3-butanediol + NAD+
-
 Saccharomyces cerevisiae (3R)-acetoin + NADH
-
 r
1.1.1.B20 2 (R,S)-acetoin + 2 NADPH + 2 H+
-
 Saccharomyces cerevisiae (2S,3S)-2,3-butanediol + meso-2,3-butanediol + NADP+ Ara1p is selective toward the acetoin carbonyl group, leading to an S-alcohol r
1.1.1.76 (R,S)-acetoin + NADPH
-
 Saccharomyces cerevisiae (2S,3S)-2,3-butanediol + meso-2,3-butanediol + NADP+ Ara1p is selective toward the acetoin carbonyl group, leading to an S-alcohol r
1.1.1.427 (R/S)-acetoin + NADPH Ara1p is as an oxidoreductase that can reduce racemic acetoin to meso-2,3-butanediol and (2S,3S)-2,3-butanediol in the presence of NADPH Saccharomyces cerevisiae (2S,3S)-2,3-butanediol + NADP+
-
 ?
1.1.1.427 additional information enzyme in addition acts as a NADPH-dependent oxidoreductase producing meso-2,3-butanediol and (2S,3S)-2,3-butanediol from (R,S)-acetoin Saccharomyces cerevisiae ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.1.1.B20 ARA1
-
 Saccharomyces cerevisiae
1.1.1.76 ARA1
-
 Saccharomyces cerevisiae
1.1.1.427 Ara1p
-
 Saccharomyces cerevisiae
1.1.1.427 D-arabinose dehydrogenase
-
 Saccharomyces cerevisiae

Expression

EC Number Organism Comment Expression
1.1.1.4 Saccharomyces cerevisiae expression is induced upon glucose depletion up

General Information

EC Number General Information Comment Organism
1.1.1.4 physiological function deletion of BDH1 results in an accumulation of acetoin and a diminution of 2,3-butanediol in two Saccharomyces cerevisiae strains under two different growth conditions Saccharomyces cerevisiae
1.1.1.B20 physiological function deletion of BDH1 results in an accumulation of acetoin and a diminution of 2,3-butanediol in two Saccharomyces cerevisiae strains under two different growth conditions Saccharomyces cerevisiae
1.1.1.76 physiological function deletion of BDH1 results in an accumulation of acetoin and a diminution of 2,3-butanediol in two Saccharomyces cerevisiae strains under two different growth conditions Saccharomyces cerevisiae
1.1.1.117 malfunction a double mutant DELATbdh1 and DELTA ara1 strain still produces (2S,3S)-2,3-butanediol and meso-2,3-butanediol suggesting only a minor role of Ara1p in the production of 2,3-butanediol Saccharomyces cerevisiae