Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Nielsen, D.R.; Yoon, S.H.; Yuan, C.J.; Prather, K.L.
    Metabolic engineering of acetoin and meso-2, 3-butanediol biosynthesis in E. coli (2010), Biotechnol. J., 5, 274-284.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.B20 gene bdh1, phylogenetic analysis, expression in Escherichia coi strain YYC202(DE3) ldhA-/- ilvC? expressing ilvBN from Escherichia coli and aldB from Lactobacillus lactis, encoding acetolactate synthase and acetolactate decarboxylase activities, respectively, disruption of the lactate biosynthesis pathway in the strain increases pyruvate precursor availability to this strain, increased availability of NADH for acetoin reduction to meso-2,3-butanediol is the most important consequence of ldhA deletion Saccharomyces cerevisiae
1.1.1.B20 phylogenetic analysis, enzyme expression in Escherichia coli strains MG1655(DE3) and YYC202(DE3) Escherichia coli
4.1.1.5 expressed in Escherichia coli YYC202(DE3) cells Lactobacillus delbrueckii subsp. lactis

Protein Variants

EC Number Protein Variants Comment Organism
1.1.1.B20 additional information expression of gene bdh1 from Saccharomyces cervisiae in Escherichia coi strain YYC202(DE3) ldhA-/- ilvC-/- expressing ilvBN from Escherichia coli and aldB from Lactobacillus lactis, encoding acetolactate synthase and acetolactate decarboxylase activities, respectively. Disruption of the lactate biosynthesis pathway in the strain increases pyruvate precursor availability to this strain, increased availability of NADH for acetoin reduction to meso-2,3-butanediol is the most important consequence of ldhA deletion. Optimization of 2,3-butanediol production in Escherichia coli, overview Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.B20 (3R)-acetoin + NADH + H+ Saccharomyces cerevisiae
-
 meso-2,3-butanediol + NAD+
-
 r
1.1.1.B20 (3R)-acetoin + NADH + H+ Escherichia coli low activity in vivo meso-2,3-butanediol + NAD+
-
 r

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.B20 Escherichia coli
-
-
-
1.1.1.B20 Saccharomyces cerevisiae
-
 gene bdh1
-
4.1.1.5 Lactobacillus delbrueckii subsp. lactis
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.B20 (3R)-acetoin + NADH + H+
-
 Escherichia coli meso-2,3-butanediol + NAD+
-
 r
1.1.1.B20 (3R)-acetoin + NADH + H+
-
 Saccharomyces cerevisiae meso-2,3-butanediol + NAD+
-
 r
1.1.1.B20 (3R)-acetoin + NADH + H+ low activity in vivo Escherichia coli meso-2,3-butanediol + NAD+
-
 r
4.1.1.5 (S)-2-hydroxy-2-methyl-3-oxobutanoate
-
 Lactobacillus delbrueckii subsp. lactis (R)-acetoin + CO2
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.1.1.B20 2,3-BD dehydrogenase
-
 Escherichia coli
1.1.1.B20 2,3-BD dehydrogenase
-
 Saccharomyces cerevisiae
4.1.1.5 aldB
-
 Lactobacillus delbrueckii subsp. lactis

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.B20 NADH
-
 Escherichia coli
1.1.1.B20 NADH
-
 Saccharomyces cerevisiae

General Information

EC Number General Information Comment Organism
1.1.1.B20 evolution phylogenetic analysis Escherichia coli
1.1.1.B20 evolution phylogenetic analysis Saccharomyces cerevisiae