Literature summary extracted from
Najmudin, S.; Andersen, J.T.; Patkar, S.A.; Borchert, T.V.; Crout, D.H.G.; Fülöp, V.
Purification, crystallization and preliminary X-ray crystallographic studies on acetolactate decarboxylase (2003), Acta Crystallogr. Sect. D, 59, 1073-1075.
Application
EC Number |
Application |
Comment |
Organism |
---|
4.1.1.5 |
nutrition |
ADC has a practical application in brewing, used to speed maturation |
Brevibacillus brevis |
Cloned(Commentary)
EC Number |
Cloned (Comment) |
Organism |
---|
4.1.1.5 |
overexpression in Bacillus subtilis JA222 |
Brevibacillus brevis |
Crystallization (Commentary)
EC Number |
Crystallization (Comment) |
Organism |
---|
4.1.1.5 |
recombinant enzyme, expressed in Bacillus subtilis JA222, hanging drop vapour-diffusion method, X-ray structure |
Brevibacillus brevis |
Organism
EC Number |
Organism |
UniProt |
Comment |
Textmining |
---|
4.1.1.5 |
Brevibacillus brevis |
- |
strain ATCC 11031 |
- |
Purification (Commentary)
EC Number |
Purification (Comment) |
Organism |
---|
4.1.1.5 |
recombinant enzyme, expressed in Bacillus subtilis JA222 |
Brevibacillus brevis |
Reaction
EC Number |
Reaction |
Comment |
Organism |
Reaction ID |
---|
4.1.1.5 |
(2S)-2-hydroxy-2-methyl-3-oxobutanoate = (3R)-3-hydroxybutan-2-one + CO2 |
mechanism |
Brevibacillus brevis |
|
Substrates and Products (Substrate)
EC Number |
Substrates |
Comment Substrates |
Organism |
Products |
Comment (Products) |
Rev. |
Reac. |
---|
4.1.1.5 |
(R)-2-hydroxy-2-methyl-3-oxobutanoate |
decarboxylates both enantiomers of acetolactate to form (R)-acetoin, decarboxylation at a lower rate than of the (S)-enantiomer, mechanism |
Brevibacillus brevis |
(R)-acetoin + CO2 |
- |
? |
|
4.1.1.5 |
(S)-2-hydroxy-2-ethyl-3-oxobutanoate |
- |
Brevibacillus brevis |
(R)-3-hydroxypentan-2-one + CO2 |
- |
? |
|
4.1.1.5 |
(S)-2-hydroxy-2-methyl-3-oxobutanoate |
decarboxylates both enantiomers of acetolactate to form (R)-acetoin, (S)-enantiomer is the normal substrate, mechanism |
Brevibacillus brevis |
(R)-acetoin + CO2 |
- |
? |
|
Synonyms
EC Number |
Synonyms |
Comment |
Organism |
---|
4.1.1.5 |
ADC |
- |
Brevibacillus brevis |
pI Value
EC Number |
Organism |
Comment |
pI Value Maximum |
pI Value |
---|
4.1.1.5 |
Brevibacillus brevis |
around |
- |
6 |