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Literature summary extracted from

  • Oshiro, T.; Aisaka, K.; Uwajima, T.
    Purification and characterization of alpha-acetolactate decarboxylase from Brevibacterium acetylicum (1989), Agric. Biol. Chem., 53, 1913-1918.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.1.1.5 1,10-phenanthroline
-
 Exiguobacterium acetylicum
4.1.1.5 8-Quinolinol
-
 Exiguobacterium acetylicum
4.1.1.5 Cu2+
-
 Exiguobacterium acetylicum
4.1.1.5 diethyldithiocarbamate
-
 Exiguobacterium acetylicum
4.1.1.5 Fe2+
-
 Exiguobacterium acetylicum
4.1.1.5 Fe2+
-
 Klebsiella aerogenes
4.1.1.5 Fe3+
-
 Exiguobacterium acetylicum
4.1.1.5 NEM weak Exiguobacterium acetylicum
4.1.1.5 PCMB weak Exiguobacterium acetylicum
4.1.1.5 Trinitrobenzenesulfonate weak Exiguobacterium acetylicum

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.1.1.5 Fe contains 0.537 nmol of Fe per mg of protein Exiguobacterium acetylicum
4.1.1.5 Zn contains 38.2 nmol of Zn per mg of protein Exiguobacterium acetylicum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.1.1.5 31000
-
 2 * 31000, SDS-PAGE Exiguobacterium acetylicum
4.1.1.5 62000
-
 gel filtration Exiguobacterium acetylicum

Organism

EC Number Organism UniProt Comment Textmining
4.1.1.5 Bacillus licheniformis
-
-
-
4.1.1.5 Exiguobacterium acetylicum
-
-
-
4.1.1.5 Klebsiella aerogenes
-
-
-
4.1.1.5 Lactiplantibacillus plantarum
-
-
-
4.1.1.5 Micrococcus luteus
-
-
-
4.1.1.5 Proteus mirabilis
-
-
-
4.1.1.5 Proteus vulgaris
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.1.1.5
-
 Exiguobacterium acetylicum

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.1.1.5 additional information
-
-
 Klebsiella aerogenes
4.1.1.5 additional information
-
-
 Exiguobacterium acetylicum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.1.1.5 2-Hydroxy-2-methyl-3-oxobutanoate
-
 Lactiplantibacillus plantarum (R)-3-Hydroxybutan-2-one + CO2
-
 ?
4.1.1.5 2-Hydroxy-2-methyl-3-oxobutanoate
-
 Klebsiella aerogenes (R)-3-Hydroxybutan-2-one + CO2
-
 ?
4.1.1.5 2-Hydroxy-2-methyl-3-oxobutanoate
-
 Proteus mirabilis (R)-3-Hydroxybutan-2-one + CO2
-
 ?
4.1.1.5 2-Hydroxy-2-methyl-3-oxobutanoate
-
 Proteus vulgaris (R)-3-Hydroxybutan-2-one + CO2
-
 ?
4.1.1.5 2-Hydroxy-2-methyl-3-oxobutanoate
-
 Micrococcus luteus (R)-3-Hydroxybutan-2-one + CO2
-
 ?
4.1.1.5 2-Hydroxy-2-methyl-3-oxobutanoate
-
 Bacillus licheniformis (R)-3-Hydroxybutan-2-one + CO2
-
 ?
4.1.1.5 2-Hydroxy-2-methyl-3-oxobutanoate
-
 Exiguobacterium acetylicum (R)-3-Hydroxybutan-2-one + CO2
-
 ?

Subunits

EC Number Subunits Comment Organism
4.1.1.5 dimer 2 * 31000, SDS-PAGE Exiguobacterium acetylicum

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
4.1.1.5 20 50 20°C: about 30% of maximal activity, 50°C: about 90% of maximal activity Exiguobacterium acetylicum

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
4.1.1.5 60
-
 rapid loss of activity above Exiguobacterium acetylicum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.1.1.5 6
-
-
 Exiguobacterium acetylicum

pH Range

EC Number pH Minimum pH Maximum Comment Organism
4.1.1.5 4 8 pH 4.0: about 25% of maximal activity, pH 8.0: about 60% of maximal activity Exiguobacterium acetylicum

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
4.1.1.5 5 8 30 min at 37°C: complete loss of activity at pH 4, about 60% loss of activity at pH 5, completely stable at pH 7, about 20% loss of activity at pH 8, complete loss of activity at pH 9 Exiguobacterium acetylicum