EC Number | Cloned (Comment) | Organism |
---|---|---|
1.1.1.4 | overexpression in Saccharomyces cerevisiae | Saccharomyces cerevisiae |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.1.1.4 | 41000 | - |
2 * 41000, SDS-PAGE | Saccharomyces cerevisiae |
1.1.1.4 | 81800 | - |
gel filtration | Saccharomyces cerevisiae |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.1.1.4 | Saccharomyces cerevisiae | - |
- |
- |
1.1.1.303 | Saccharomyces cerevisiae | P39714 | bifunctional diacetyl reductase and (R)-2,3-butanediol dehydrogenase | - |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.1.1.4 | - |
Saccharomyces cerevisiae |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
1.1.1.4 | 968 | - |
- |
Saccharomyces cerevisiae |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.1.1.4 | (2R,3R)-2,3-butanediol + NAD+ | - |
Saccharomyces cerevisiae | (3R)-acetoin + NADH | - |
r | |
1.1.1.4 | (3R,3S)-acetoin + NADH | - |
Saccharomyces cerevisiae | (2R,3R)-2,3-butanediol + meso-2,3-butanediol + NAD+ | - |
r | |
1.1.1.4 | 2,3-pentanediol + NAD+ | - |
Saccharomyces cerevisiae | 4-hydroxy-3-pentanone + 3-hydroxy-2-pentanone + NADH | - |
? | |
1.1.1.4 | meso-2,3-butanediol + NAD+ | - |
Saccharomyces cerevisiae | D-(-)-acetoin + NADH | (3S)-acetoin | r | |
1.1.1.4 | additional information | 3S)-2,3-butanediol, (3R/3S)-acetoin, glycerol, sorbitol or xylitol are no substrates | Saccharomyces cerevisiae | ? | - |
? | |
1.1.1.303 | diacetyl + NADH + H+ | 51.4% of the (R)-2,3-butanediol dehydrogenase activity with substrate acetoin | Saccharomyces cerevisiae | (R)-acetoin + NAD+ | - |
ir |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.1.1.4 | dimer | 2 * 41000, SDS-PAGE | Saccharomyces cerevisiae |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.1.1.4 | 7 | - |
acetoin reduction | Saccharomyces cerevisiae |
1.1.1.4 | 8 | - |
substrate oxidation | Saccharomyces cerevisiae |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.1.1.4 | NAD+ | no activity with NADP+ as cofactor | Saccharomyces cerevisiae | |
1.1.1.303 | NADH | - |
Saccharomyces cerevisiae |
EC Number | General Information | Comment | Organism |
---|---|---|---|
1.1.1.303 | physiological function | gene disruption is not lethal. Disrupted strains can grow on 2,3-butanediol, but to a lesser cell density than wild-type | Saccharomyces cerevisiae |