Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Höhn-Bentz, H.; Radler, F.
    Bacterial 2,3-butanediol dehydrogenases (1978), Arch. Microbiol., 116, 197-203.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.4 Borate buffer
-
 Paenibacillus polymyxa

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.4 0.1
-
 NADH
-
 Paenibacillus polymyxa
1.1.1.4 0.2
-
 NAD+
-
 Paenibacillus polymyxa
1.1.1.4 0.53
-
 acetoin
-
 Paenibacillus polymyxa
1.1.1.4 3.3
-
 D-(-)2,3-butanediol
-
 Paenibacillus polymyxa
1.1.1.4 6.25
-
 meso-2,3-butanediol
-
 Paenibacillus polymyxa
1.1.1.4 38
-
 1,2-propanediol
-
 Paenibacillus polymyxa
1.1.1.4 87
-
 diacetyl
-
 Paenibacillus polymyxa

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.1.1.4 additional information no requirement Bacillus subtilis
1.1.1.4 additional information no requirement Paenibacillus polymyxa
1.1.1.4 additional information no requirement Aeromonas hydrophila
1.1.1.4 additional information no requirement Gluconobacter oxydans

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.4 100000 107000 gel filtration Paenibacillus polymyxa

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.4 Aeromonas hydrophila
-
-
-
1.1.1.4 Bacillus subtilis
-
-
-
1.1.1.4 Gluconobacter oxydans
-
-
-
1.1.1.4 Paenibacillus polymyxa
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.4
-
 Paenibacillus polymyxa

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.4 0.21
-
-
 Paenibacillus polymyxa
1.1.1.4 0.6
-
-
 Gluconobacter oxydans

Storage Stability

EC Number Storage Stability Organism
1.1.1.4 stable at -18°C Paenibacillus polymyxa

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.4 1,2-propanediol + NAD+
-
 Paenibacillus polymyxa ?
-
 r
1.1.1.4 2,3-butanediol + NAD+ D(-)-2,3-butandiol Bacillus subtilis acetoin + NADH
-
 r
1.1.1.4 2,3-butanediol + NAD+ D(-)-2,3-butandiol Paenibacillus polymyxa acetoin + NADH
-
 r
1.1.1.4 2,3-butanediol + NAD+ D(-)-2,3-butandiol Aeromonas hydrophila acetoin + NADH
-
 r
1.1.1.4 2,3-butanediol + NAD+ D(-)-2,3-butandiol Gluconobacter oxydans acetoin + NADH
-
 r
1.1.1.4 acetoin + NADH + H+
-
 Bacillus subtilis 2,3-butanediol + NAD+
-
 r
1.1.1.4 acetoin + NADH + H+
-
 Paenibacillus polymyxa 2,3-butanediol + NAD+
-
 r
1.1.1.4 acetoin + NADH + H+
-
 Aeromonas hydrophila 2,3-butanediol + NAD+
-
 r
1.1.1.4 acetoin + NADH + H+
-
 Gluconobacter oxydans 2,3-butanediol + NAD+
-
 r
1.1.1.4 diacetyl + NADH + H+
-
 Paenibacillus polymyxa acetoin + NAD+
-
 r
1.1.1.4 meso-2,3-butanediol + NAD+
-
 Paenibacillus polymyxa ?
-
 r

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.1.4 60 62
-
 Paenibacillus polymyxa
1.1.1.4 60 62
-
 Gluconobacter oxydans

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.1.1.4 60 65
-
 Paenibacillus polymyxa

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.4 7
-
 acetoin reduction Gluconobacter oxydans
1.1.1.4 9
-
 D(-)-2,3-butanediol oxidation Gluconobacter oxydans

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.1.1.4 7 7.6
-
 Paenibacillus polymyxa

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.4 NAD+
-
 Bacillus subtilis
1.1.1.4 NAD+
-
 Paenibacillus polymyxa
1.1.1.4 NAD+
-
 Aeromonas hydrophila
1.1.1.4 NAD+
-
 Gluconobacter oxydans
1.1.1.4 NADH
-
 Bacillus subtilis
1.1.1.4 NADH
-
 Paenibacillus polymyxa
1.1.1.4 NADH
-
 Aeromonas hydrophila
1.1.1.4 NADH
-
 Gluconobacter oxydans