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Literature summary for 6.3.4.16 extracted from
- Structural insight on the control of urea synthesis: identification of the binding site for N-acetyl-L-glutamate, the essential allosteric activator of mitochondrial carbamoyl phosphate synthetase (2009), Biochem. J., 424, 211-220.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| inosine monophosphate | weak activator, the IMP binding site is located at the C-terminus, binding structure analysis from crystal structure with bound IMP, PDB ID 1CE8 | Escherichia coli |  |
| N-acetyl-L-beta-phenylglutamate | lower activation of CPSI compared to N-acetyl-L-glutamate | Homo sapiens | |
| N-acetyl-L-glutamate | i.e. NAG, allosteric activator, binding mode and site structure analysis by MALDI-TOF mass spectrometry, overview | Rattus norvegicus | |
| N-acetyl-L-glutamate | i.e. NAG, allosteric activator, the binding mode and site in the crystal structure of human CPSI, PDB ID 2YVQ, is located in the C-terminal domain, docking experiments and structure, overview | Homo sapiens | |
| ornithine | - |
Escherichia coli | |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| cloning of N-terminally His6-tagged wild-type and mutant CPSIs in Escherichia coli and expression in Spodopetra frugiperda SF9 cells | Rattus norvegicus |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| N1437D | site-directed mutagenesis, the mutant enzymes shows reduced activation by N-acetyl-L-glutamate compared to the wild-type enzyme | Rattus norvegicus |
| N1440D | site-directed mutagenesis, the mutant enzymes shows reduced activation by N-acetyl-L-glutamate compared to the wild-type enzyme | Rattus norvegicus |
| T1391V | site-directed mutagenesis, the mutant enzymes shows reduced activation by N-acetyl-L-glutamate compared to the wild-type enzyme | Rattus norvegicus |
| T1394A | site-directed mutagenesis, the mutant enzymes shows reduced activation by N-acetyl-L-glutamate compared to the wild-type enzyme | Rattus norvegicus |
| W1410K | site-directed mutagenesis, the mutant binds N-acetyl-L-beta-phenylglutamate at the N-acetyl-L-glutamate binding site | Homo sapiens |
| W1410K | site-directed mutagenesis, the mutant enzymes shows reduced activation by N-acetyl-L-glutamate compared to the wild-type enzyme | Rattus norvegicus |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| mitochondrion | - |
Rattus norvegicus | 5739 | - |
| mitochondrion | - |
Homo sapiens | 5739 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | - |
Escherichia coli | |
| Mg2+ | - |
Rattus norvegicus | |
| Mg2+ | - |
Homo sapiens | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2 ATP + NH3 + CO2 + H2O | Escherichia coli | - |
2 ADP + phosphate + carbamoyl phosphate | - |
? | |
| 2 ATP + NH3 + CO2 + H2O | Rattus norvegicus | - |
2 ADP + phosphate + carbamoyl phosphate | - |
? | |
| 2 ATP + NH3 + CO2 + H2O | Homo sapiens | - |
2 ADP + phosphate + carbamoyl phosphate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | - |
- |
- |
| Homo sapiens | P31327 | - |
- |
| Rattus norvegicus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant His6-tagged wild-type and mutant CPSIs from Spodoptera frugiperda SF9 cells by affinity chromatography | Rattus norvegicus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| liver | - |
Rattus norvegicus | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2 ATP + NH3 + CO2 + H2O | - |
Escherichia coli | 2 ADP + phosphate + carbamoyl phosphate | - |
? | |
| 2 ATP + NH3 + CO2 + H2O | - |
Rattus norvegicus | 2 ADP + phosphate + carbamoyl phosphate | - |
? | |
| 2 ATP + NH3 + CO2 + H2O | - |
Homo sapiens | 2 ADP + phosphate + carbamoyl phosphate | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| carbamoyl phosphate synthetase | - |
Escherichia coli |
| carbamoyl phosphate synthetase | - |
Rattus norvegicus |
| carbamoyl phosphate synthetase | - |
Homo sapiens |
| carbamoyl phosphate synthetase I | - |
Rattus norvegicus |
| carbamoyl phosphate synthetase I | - |
Homo sapiens |
| CPS | - |
Escherichia coli |
| CPSI | - |
Rattus norvegicus |
| CPSI | - |
Homo sapiens |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Escherichia coli |
| 37 | - |
assay at | Rattus norvegicus |
| 37 | - |
assay at | Homo sapiens |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.4 | - |
assay at | Escherichia coli |
| 7.4 | - |
assay at | Rattus norvegicus |
| 7.4 | - |
assay at | Homo sapiens |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| ATP | - |
Escherichia coli | |
| ATP | - |
Rattus norvegicus | |
| ATP | - |
Homo sapiens | |
General Information
| General Information | Comment | Organism |
|---|---|---|
| metabolism | CPSI is the first urea cycle enzyme | Rattus norvegicus |
| metabolism | CPSI is the first urea cycle enzyme | Homo sapiens |
| physiological function | CPSI is a key regulator of the urea cycle for ammonia detoxification in animals | Rattus norvegicus |
| physiological function | CPSI is a key regulator of the urea cycle for ammonia detoxification in animals, including humans | Homo sapiens |
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